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‫اػبًغ‬ ‫اص‬ ُ‫اػتفبد‬ ‫ثب‬ ُ‫ؿذ‬ ‫ثبیگبًی‬ ‫اػٌبد‬ ‫کشدى‬ ‫ػفًَی‬ ‫ضذ‬
‫پبییي‬ ‫دهبی‬ ‫ثب‬ ‫پالػوب‬ ٍ ُ‫ًقش‬ ‫رسات‬ ًَ‫ًب‬ ‫پَؿؾ‬ ،‫آٍیـي‬
‫ّذف‬‫ایي‬،‫پظٍّؾ‬‫تؼییي‬‫احشثخـی‬‫سٍؽ‬‫ّبی‬‫ضذػفًَی‬‫ثب‬‫اػبًغ‬
‫آٍیـي‬(TEO)،‫پَؿؾ‬‫رسات‬ُ‫ًقش‬(AgNPS)ٍ‫پالػوبی‬‫دهبی‬
‫پبییي‬(LTP)‫تَػظ‬‫سٍؽ‬ِ‫ٍاثؼت‬ِ‫ث‬‫کـت‬‫هیکشٍة‬‫آصهبیـگبّی‬ٍ‫تحلیل‬
RNA‫اػت‬.
‫ّوچٌیي‬‫تبحیش‬‫ضذػفًَی‬‫ثش‬‫خَاف‬‫هکبًیکی‬ٍ‫ًَسی‬‫یک‬‫ثشگ‬‫کبغز‬‫اص‬
‫کتبة‬‫ّبی‬‫تبسیخی‬‫ثب‬‫ػغَح‬‫هختلف‬‫اص‬‫آلَدگی‬‫هیکشٍثی‬‫هَسد‬‫ثشسػی‬‫قشاس‬
‫گشفت‬.
‫پظٍّؾ‬ ‫اّذاف‬(..ِ‫اداه‬)
ِ‫هقذه‬
‫تشکیجبت‬‫ؿیویبیی‬‫هَاسد‬‫ثبیگبًی‬ُ‫ؿذ‬ٍِ‫هجوَػ‬‫ّبی‬ًِ‫کتبثخب‬‫ای‬‫ؿبهل‬،‫کبغز‬،‫چشم‬
‫کبغز‬،‫پَػتی‬،‫چؼت‬،ِ‫پبسچ‬‫هْش‬،‫هَهی‬ٍ‫ػکغ‬‫ّب‬‫ثبػج‬‫هی‬‫ؿَد‬ِ‫ک‬ِ‫ث‬‫ػبدگی‬
‫تَػظ‬ٍ‫هیکش‬‫اسگبًیؼن‬‫ّب‬ُ‫آلَد‬‫ؿًَذ‬ِ‫ث‬ُ‫ٍیظ‬‫دس‬‫ؿشایغی‬ِ‫ک‬‫سعَثت‬‫صیبد‬‫ثبؿذ‬.‫دس‬
‫ػشاػش‬،‫جْبى‬‫تخشیت‬‫کتبة‬‫ّب‬‫ًبؿی‬‫اص‬‫فؼبلیت‬ٍ‫هیکش‬‫اسگبًیؼن‬،‫ّب‬‫یک‬‫هـکل‬
ِ‫ؿٌبخت‬ُ‫ؿذ‬‫دس‬ِ‫ًَؿت‬‫ّب‬ٍ‫ػول‬‫هحبفظت‬‫اص‬‫آى‬‫ّب‬‫اػت‬ِ‫ک‬‫ؿبهل‬ِ‫تجضی‬،‫پَدسی‬
‫ػفت‬‫ؿذى‬ٍُ‫پذیذ‬Foxing(‫ایجبد‬ِ‫ًقغ‬‫ّبی‬ُ‫پشاکٌذ‬ِ‫ک‬‫هؼوَال‬‫قشهض‬ٍَُْ‫ق‬‫ای‬
‫ّؼتٌذ‬ٍِ‫ث‬‫دلیل‬‫فؼبلیت‬‫قبسچ‬‫ّب‬ِ‫ث‬‫ٍجَد‬‫هی‬‫آیٌذ‬)‫هی‬‫ؿَد‬.‫ػَخت‬ٍ‫ػبص‬
‫هیکشٍثی‬‫هی‬‫تَاًذ‬‫ػجت‬‫تغییش‬،‫سًگ‬،ِ‫تجضی‬‫تقلیل‬‫سفتي‬‫اػتحکبم‬،‫کبغز‬ٍ‫ایجبد‬
‫ثَی‬‫خبف‬‫کپک‬‫ؿَد‬ِ‫ک‬‫خَد‬‫ػجت‬‫یک‬‫صیبى‬‫غیش‬‫قبثل‬‫ثبصگـت‬ِ‫ث‬‫هیشاث‬‫فشٌّگی‬
‫هی‬‫ؿَد‬ٍ‫یک‬‫تْذیذ‬‫جذی‬‫ثشای‬‫ػالهت‬‫کبسکٌبًی‬ِ‫ک‬‫ثب‬‫هَاد‬ُ‫آلَد‬‫دس‬‫توبػٌذ‬.
ُ‫ؿذ‬ ‫ثبیگبًی‬ ‫هَاد‬ ‫ثشای‬ ‫جبیگضیي‬ ‫ضذػفًَی‬ ‫سٍؽ‬ ِ‫ػ‬
ِ‫ث‬‫دلیل‬‫ػشعبى‬‫صا‬ٍ‫آصاس‬ُ‫دٌّذ‬‫ثَدى‬‫سٍؽ‬‫ّبی‬‫هشػَم‬‫ضذػفًَی‬
‫هبًٌذ‬‫ثخَس‬‫دادى‬‫ثب‬‫اتیلي‬‫اکؼبیذ‬‫یب‬،‫فشهبلذّیذ‬‫کـَسّبی‬ِ‫اتحبدی‬‫اسٍپب‬ِ‫ث‬
‫تذسیج‬‫دس‬‫حبل‬‫هتَقف‬‫کشدى‬‫ایي‬‫سٍؽ‬‫ّب‬‫ّؼتٌذ‬.‫سٍؽ‬‫ّبی‬‫دیگش‬‫چَى‬َ‫پشت‬
‫گبهب‬ٍ‫فشاثٌفؾ‬‫ّن‬‫ًوی‬‫تَاًٌذ‬‫ثشای‬‫ضذػفًَی‬‫اؿیب‬‫تبسیخی‬ُ‫اػتفبد‬‫ؿًَذ‬
‫چَى‬‫ثبػج‬‫کبّؾ‬‫قبثل‬ِ‫تَج‬‫اػتحکبم‬ٍ‫تغییش‬‫سًگ‬‫کبغز‬‫هی‬‫ؿًَذ‬.
ُ‫ؿذ‬ ِ‫پشداخت‬ ‫جبیگضیي‬ ‫ضذػفًَی‬ ‫سٍؽ‬ ِ‫ػ‬ ‫ثشسػی‬ ِ‫ث‬ ِ‫هغبلؼ‬ ‫ایي‬ ‫دس‬
‫اػت‬:
‫اػبًغ‬‫آٍیـي‬(TEO)
ُ‫ًقش‬ ‫رسات‬ ًَ‫ًب‬ ‫پَؿؾ‬(AgNPs)
‫پبییي‬ ‫دهبی‬ ‫پالػوبی‬(LTP)
(TEO)‫آٍیـي‬ ‫اػبًغ‬
،ُ‫اهشٍص‬‫اػبًغ‬‫ّب‬ِ‫ث‬‫ؿکل‬‫هَفقیت‬‫آهیضی‬‫دس‬ٌِ‫صهی‬‫ّبی‬‫هختلف‬‫لَاصم‬
‫آسایـی‬ٍ،‫ثْذاؿتی‬‫صٌبیغ‬‫غزایی‬ٍ‫ّچٌیي‬‫دسهبى‬‫ػفًَت‬ٍ‫ثیوبسی‬‫ّب‬
ُ‫اػتفبد‬ُ‫ؿذ‬‫اًذ‬.
‫احشایي‬‫هَاد‬‫دس‬‫ثشاثش‬‫اًَاع‬‫هختلف‬‫اسگبًیؼن‬‫ّب‬‫اص‬ِ‫جول‬‫ٍیشٍع‬،‫ّب‬
‫ثبکتشی‬،‫ّب‬‫قبسچ‬،‫ّب‬‫تک‬ِ‫یبخت‬ٍ‫حـشات‬ِ‫ث‬‫خَثی‬ِ‫ؿٌبخت‬ُ‫ؿذ‬‫اًذ‬.
ِ‫اگشچ‬‫هکبًیؼن‬‫ضذ‬‫هیکشٍثی‬‫اػبًغ‬‫ّب‬ِ‫ث‬ُ‫اًذاص‬‫کبفی‬ِ‫ؿٌبخت‬ُ‫ؿذ‬
،‫ًیؼت‬‫اهب‬ِ‫ث‬‫پبساهتشّبی‬،‫ثؼیبسی‬‫اص‬ِ‫جول‬‫تشکیجبت‬‫ؿیویبیی‬‫ثؼتگی‬
‫داسد‬.‫فؼبلیت‬‫ضذ‬‫هیکشٍثی‬‫اجضای‬‫اػبًغ‬‫ّب‬‫سا‬‫هی‬‫تَاى‬ِ‫ث‬‫تشتیت‬‫ًضٍلی‬
‫صیش‬ِ‫دػت‬‫ثٌذی‬‫کشد‬:
‫فٌَل‬‫ّب‬>‫آلذّیذّب‬>‫کتَى‬‫ّب‬>‫الکل‬‫ّب‬>‫اػتشّب‬>‫ّیذسٍکشثي‬‫ّب‬
ُ‫ًقش‬ ‫رسات‬ ًَ‫ًب‬ ‫پَؿؾ‬(AGNPS)
‫ًبًَرسات‬ُ‫ًقش‬AgNPs‫ثشای‬‫خَاف‬‫ضذ‬‫هیکشٍثی‬‫خَد‬ِ‫ؿٌبخت‬
ُ‫ؿذ‬‫اًذ‬.‫پیؾ‬‫اص‬‫ایي‬‫ثشای‬‫ضذػفًَی‬‫هَاد‬‫تبسیخی‬‫هبًٌذ‬‫کبغز‬
،‫پَػتی‬،‫چشم‬،‫چَة‬،‫الیبف‬‫ػٌگ‬‫ّبی‬‫ثبػتبى‬‫ؿٌبػی‬ٍ‫کبغز‬ِ‫ث‬
ُ‫کبسثشد‬ُ‫ؿذ‬‫اًذ‬.‫ّیچ‬‫تبحیش‬‫هٌفی‬‫تَػظ‬AgNPs‫ثش‬‫سٍی‬‫خَاف‬
‫هَادی‬ِ‫ک‬‫هجتٌی‬‫ثش‬‫کبغز‬‫ّؼتٌذ‬ُ‫هـبّذ‬ُ‫ًـذ‬‫اػت‬.
‫پبییي‬ ‫دهبی‬ ‫پالػوبی‬(LTP)
‫پالػوبی‬‫دهبی‬‫پبییي‬(LTP)‫سا‬‫هی‬‫تَاى‬‫ثب‬ِ‫تخلی‬‫ی‬‫الکتشیکی‬‫دس‬،‫گبصّب‬
‫تحت‬‫فـبس‬‫کن‬‫یب‬‫جَی‬‫تَلیذ‬‫کشد‬.‫اتوؼفش‬‫ّبیی‬ِ‫ک‬‫اغلت‬ُ‫اػتفبد‬‫هی‬‫ؿًَذ‬
‫َّای‬‫عجیؼی‬‫یب‬‫تشکیجی‬،‫ًیتشٍطى‬،‫اکؼیظى‬،‫ّلیَم‬،‫ّیذسٍطى‬‫آسگَى‬ٍ‫هخلَط‬
‫آى‬‫ّبػت‬.
‫ػَاهل‬‫فؼبل‬‫تَلیذ‬ُ‫ؿذ‬‫تَػظ‬‫پالػوب‬‫دس‬‫سعَثت‬،‫ثبال‬‫اًَاع‬‫اکؼیظى‬‫ٍاکٌـی‬
(ROS)‫هبًٌذ‬O،O2،O2-،O3،‫اًَاع‬‫ًیتشٍطى‬‫ٍاکٌـی‬(RNS)‫هبًٌذ‬N،
N2،NO‫ّوچٌیي‬‫اًَاع‬‫ّیذسٍطى‬‫ٍاکٌـی‬H2O،OH-،OH،H2O2
‫ّؼتٌذ‬.
‫اص‬LTP‫اخیشا‬‫ثشای‬‫ضذػفًَی‬‫هیکشٍثی‬‫ػغَح‬ٍ‫هبیغ‬‫ّب‬‫هبًٌذ‬:،‫فبضالة‬
‫غزاّبی‬،ُ‫آهبد‬‫هَاد‬ِ‫ثؼت‬‫ثٌذی‬‫غزایی‬ٍ‫ّوچٌیي‬‫ثشای‬‫تویض‬‫کشدى‬‫اجؼبم‬
‫ثبػتبى‬‫ؿٌبػی‬ُ‫اػتفبد‬ُ‫ؿذ‬‫اػت‬.
‫ّب‬ ‫سٍؽ‬ ٍ ‫هَاد‬
‫ّب‬ ‫کتبة‬
‫هیکشٍثی‬ ‫آلَدگی‬ ‫تحلیل‬ ٍ ِ‫تجضی‬
-‫ّبی‬ ‫تحلیل‬ ٍ ِ‫تجضی‬‫ّی‬‫و‬‫ک‬
-‫غلظت‬RNA
-‫ؿٌبػبیی‬ِ‫ایضٍل‬ ‫ّبی‬ ‫هیکشٍة‬ُ‫ؿذ‬
‫کبغز‬ ‫ّبی‬ ‫ٍیظگی‬ ‫اسصیبثی‬
‫ػفًَی‬ ‫ضذ‬
‫سیبضی‬ ‫هحبػجبت‬
‫آهبسی‬ ‫تحلیل‬ ٍ ِ‫تجضی‬
‫ثشسػی‬ ٍ ‫ثحج‬
‫سٍؽ‬‫ثب‬ ِ‫هقبیؼ‬ ‫دس‬ ٍ ُ‫ثَد‬ ‫ثبکتشی‬ ‫ضذ‬ ‫ثیـتش‬ ُ‫ؿذ‬ ُ‫اػتفبد‬ ‫ضذػفًَی‬ ‫ّبی‬
‫داؿتٌذ‬ ‫ّب‬ ‫ثبکتشی‬ ‫ثش‬ ‫ثیـتشی‬ ‫تبحیش‬ ‫ّب‬ ‫قبسچ‬.
‫تشتیت‬ ِ‫ث‬ ‫ضذػفًَی‬ ‫سٍؽ‬ ‫تبحیشگزاستشیي‬:
-ًَ‫ًب‬‫رسات‬ُ‫ًقش‬(‫کبّؾ‬‫ػغح‬60‫تب‬100‫دسصذ‬)ِ‫جٌج‬‫هْن‬ِ‫ه‬‫اًذٍدکشدى‬
‫ثب‬‫رسات‬ُ‫ًقش‬‫احش‬‫آى‬‫ثش‬‫ػالهت‬‫اًؼبى‬‫اػت‬.‫ایي‬‫سٍؽ‬‫ّن‬‫ثشای‬‫هحیظ‬ٍ‫ّن‬‫ثشای‬
‫کبسکٌبًی‬ِ‫ک‬‫دس‬‫اتبق‬‫هیکشٍة‬‫صدایی‬‫فؼبلیت‬‫هی‬‫کٌٌذ‬‫ثی‬‫خغش‬‫اػت‬.
-LTP(25‫تب‬100‫دسصذ‬)
-TEO(12‫تب‬100‫دسصذ‬)
‫دس‬‫ّش‬ِ‫ػ‬‫سٍؽ‬‫احش‬‫ثش‬‫قبسچ‬‫ّب‬‫کوتش‬ُ‫ثَد‬‫اهب‬ُ‫ػصبس‬‫آٍیـي‬‫ًؼجت‬ِ‫ث‬‫ًبًَرسات‬
ُ‫ًقش‬ٍLTP‫عیف‬‫ٍػغ‬‫تشی‬‫اص‬‫فؼبلیتْبی‬‫قبسچی‬‫سا‬‫هَسد‬‫ّذف‬‫قشاس‬‫هی‬‫دّذ‬.
ٍُ‫ػال‬‫ثش‬‫ایي‬‫حتی‬‫هی‬‫تَاى‬‫اص‬ُ‫ػصبس‬‫ّبی‬،‫هیخک‬،‫اػغَخَدٍع‬،ًَِ‫پ‬‫دسخت‬
‫چبی‬ٍ‫ػبیش‬‫گیبّبى‬ِ‫ک‬‫تشکیت‬‫ػٌبصش‬‫اصلی‬‫آًْب‬،‫لیٌبلَل‬‫لیٌبلیل‬،‫اػتبت‬،‫اٍطًَل‬
‫تَطٍى‬ٍ‫ػیٌئَل‬‫هی‬‫ثبؿذ‬‫ثشای‬‫تَقف‬‫سؿذ‬‫قبسچ‬‫ثش‬‫سٍی‬‫اػٌبد‬ٍ‫هحیظ‬‫ّبی‬
‫ًگْذاسی‬‫آًْب‬‫دس‬ًِ‫کتبثخب‬‫ّب‬ٍ‫آسؿیَّب‬ُ‫اػتفبد‬‫ًوَد‬.
‫صدایی‬ ‫هیکشٍة‬ ‫احشگزاسی‬
‫داسد‬ ‫ثؼتگی‬ ‫ّن‬ ‫ثشداسی‬ ًَِ‫ًو‬ ‫هحیظ‬ ِ‫ث‬ ‫صدایی‬ ‫هیکشٍة‬ ‫احشگزاسی‬:
-‫کتبة‬ ‫اثتذای‬ ‫ػفیذ‬ ِ‫صفح‬ ٍ ‫جلذ‬ ‫سٍی‬ ‫ثش‬ ‫احش‬ ‫ثبالتشیي‬
-‫اػت‬ ُ‫ثَد‬ ‫کتبة‬ ‫صفحبت‬ ‫داخل‬ ٍ ‫ّب‬ ِ‫حبؿی‬ ‫سٍی‬ ‫ثش‬ ‫احش‬ ‫تشیي‬ ‫پبییي‬.
‫هیکشٍػکَپی‬ ‫هَجَدات‬ ‫سؿذ‬ ‫ثشای‬ ‫هکبى‬ ‫تشیي‬ ‫سایج‬:
-،‫چؼت‬ ‫هخل‬ ‫آى‬ ‫دس‬ ُ‫ؿذ‬ ُ‫اػتفبد‬ ‫هختلف‬ ‫هَاد‬ ‫هیبى‬ ‫فضبی‬ ٍ ‫کتبة‬ ‫جلذ‬
‫چشهی‬ ٍ ‫ای‬ ِ‫پبسچ‬ ‫جلذّبی‬
‫ثب‬ ‫هحیغی‬ ‫دس‬ ٍ ُ‫ثَد‬ ‫صیبدی‬ ‫هیکشٍػکَپی‬ ‫هَجَدات‬ ‫داسای‬ ِ‫ک‬ ‫ّبیی‬ ‫کتبة‬
‫ثَدین‬ ‫ؿبّذ‬ ‫سا‬ ‫ثبالتشی‬ ‫احشگزاسی‬ ‫اػت‬ ُ‫ؿذ‬ ‫هی‬ ‫ًگْذاسی‬ ‫ثبال‬ ‫سعَثت‬.
‫ثبص‬ ‫صدایی‬ ‫هیکشٍة‬ ‫فشایٌذ‬ ‫حیي‬ ‫دس‬ ‫ّب‬ ‫کتبة‬ ،‫ثیـتش‬ ‫احشگزاسی‬ ‫جْت‬ ‫اػت‬ ‫ثْتش‬
‫ؿًَذ‬ ِ‫گزاؿت‬.
‫هیکشٍة‬ ‫احشگزاسی‬‫صدایی‬(...ِ‫اداه‬)
ُ‫ؿذ‬ ‫صدایی‬ ‫هیکشٍة‬ ‫کبغزّبی‬ ‫ًَسی‬ ‫ٍیظگیْبی‬ ‫ثش‬ ،ُ‫ؿذ‬ ُ‫اػتفبد‬ ‫ّبی‬ ‫سٍؽ‬
‫داسد‬ ‫اًذکی‬ ‫تبحیش‬ ‫یب‬ ‫ًذاؿتٌذ‬ ‫تبحیش‬ ‫یب‬ ‫سًگ‬ ‫عیف‬ ٍ ‫صسدی‬ ،‫ػفیذی‬ ‫هخل‬.
‫ثؼتگی‬ ‫ّن‬ ‫هیکشٍػکَپی‬ ‫هَجَدات‬ ًَِ‫گ‬ ِ‫ث‬ ‫ضذػفًَی‬ ‫فشایٌذ‬ ‫احشگزاسی‬ ‫ػغح‬
‫داسد‬.
‫حؼبػیت‬ ٍ ‫ثَدًذ‬ ُ‫ؿذ‬ ‫جذا‬ ‫ّب‬ ‫هیکشٍاسگبًیؼن‬ ِ‫ک‬ ‫جبیی‬ ‫ػغح‬ ِ‫ث‬ ‫ّوچٌیي‬
‫داسد‬ ‫ثؼتگی‬ ‫فؼبل‬ ‫ػَاهل‬ ِ‫ث‬ ‫آًْب‬.
‫افضایؾ‬ ‫ثبػج‬ ‫تحلیل‬ ‫هَسد‬ ‫تبسیخی‬ ‫ّبی‬ ‫کتبة‬ ٍ‫د‬ ‫ّش‬ ‫اص‬ ‫کبغز‬ ‫صدایی‬ ‫هیکشٍة‬
‫ؿذ‬ ‫کبغز‬ ‫ضخبهت‬ ‫افضایؾ‬ ‫ػلت‬ ِ‫ث‬ ‫آًْب‬ ‫حجن‬.
‫صٍال‬‫کبغز‬‫یک‬‫فشایٌذ‬‫غیشقبثل‬‫ثشگـت‬‫اػت‬‫لیکي‬‫تَقف‬‫صٍال‬‫ثیـتش‬
ِ‫ث‬ِ‫ٍػیل‬‫هیکشٍة‬‫صدایی‬ٍ‫اػیذصدایی‬‫هی‬‫تَاًذ‬‫ثبػج‬‫حفظ‬‫کبغز‬
‫ؿَد‬ٍ‫صٍال‬‫ثیـتش‬‫کبغز‬‫دس‬‫ایي‬ِ‫هشحل‬ِ‫ث‬‫هٌظَس‬‫حفظ‬‫کبغزّبی‬
‫کتت‬‫تبسیخی‬‫ضشٍسی‬‫اػت‬.
‫ؿوب‬ ِ‫تَج‬ ‫اص‬ ‫ػپبع‬

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