6. Can Asparagus
inhibit population
growth in yeast cells?
Yeast Activity Viable Colony
Growth with Counts with Agar
Spectrophotometer Plates

7. Positive (YPD+)
• 1% Yeast Extract
• 2% Peptone
• 1% Dextrose
Negative (YPD-)
• Water
Experimental (YPDAsp)
• 1% Yeast Extract
• 2% Peptone
• 1% Dextrose
• 50mL of Filtered Asparagus

8. Grow S. cerevisiae Incubated all Centrifuge all
in YPD+, YPD-, cultures for the different
YPDAsp Broths hours mediums
Measured the
absorbance of each
using a Compared the Yeast
spectrophotometer Activity Growth Results
at

9. For my agar plates, Streak onto my
I inoculated a Incubate for three different
colony from a plates: positive,
positive plate hours at
negative and
colony. experimental
Incubate for Record Viable Colony
hours at Count Results

11. 1
0.9
0.8
Absorbance (600nm)
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
0 2 4 6 8 10 12
Sample Numbers

16. Experimental Results

18. Positive Experimental Negative
• Raised, Convex • Flat Surface • Produced no
Surface colonies