Water borne diseases.pptx

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swarnatrishasaha1

water borne diseases

Health & Medicine

GLOBAL BURDEN
According to cdc,
Globally 7000 deaths
120000 hospitalization
7 million illness

WATER BORNE PATHOGEN
• Vibrio cholerae
• Salmonella typhi
• Salmonella paratyphi
• Shigella spp
• Diarrhoegenic Escherichia coli
• Non tuberculosis mycobacterium
VIRUS
PARASIT
E
BACTERI
A
• Rota virus
• Adeno virus
• Hepatitis
virus A
• Hepatitis
virus E
• Polio virus
• Entamoeba
histolytica
• Giardia lamblia
• Cryptosporidium
spp. & Cyclospora
spp.
• Roundworm
• Threadworm
• Schistosoma spp.

DIARRHOEAL DISEASE PATHWAYS: FECO-
ORAL TRANSMISSION

DIARRHOEAL WATER BORNE DISEASE
CLINICAL MANIFESTATIONS
• Diarhoea
• Fever
• Jaundice
• Abdominal pain
• Nausea and vomiting

COMPLICATION
• Electrolyte irregularities
• Acute renal failure
• Encephalopathy
• Hypovolemic shock
• Hemolytic–uremic syndrome
• Seizures
• Liver, heart, or lung disease.

SPECIMEN COLLECTION & TRANSPORT
• Fecal specimen - clean, screw capped, wide mouth container.
• Other samples like rectal swab, vomitus or suspected food material can be taken if history suggests, duodenal aspirate in
giardia
• Before antibiotics
• Transport medium (cary blair, vr etc) to use – if delay >2hr
• Three specimens on consecutive days – because organisms may be shed intermittently
• Stool for the following examination should be sent without added preservatives or liquids :-
 Direct wet-mount examination
 C. Difficile toxin assay
 Immunoelectron microscopy for detection of viruses
 ELISA or latex agglutination for rotaviruses

LAB DIAGNOSIS OF DIARHOEAL DISEASE
• Sample- Stool
Vomitus
rectal swab
• Transport – should be immediately as soon as possible.
Cary-Blair transport medium
Alkalline peptone water
Alkalline peptone
water
Cary blair
medium

CULTURE METHODS
• FOR SALMONELLA/ SHIGELLA:
PLATING MEDIA
MILDLY SELECTIVE -
MACCONKEY AGAR
HIGHLY SELECTIVE - XLD, DCA
AGAR
ENRICHMENT BROTH - SELENITE F
• FOR V. CHOLERAE, USE: BLOOD AGAR, TCBS (SELECTIVE),
ALKALINE PEPTONE WATER

E.Coli in XLD
Selenite F broth Alkaline
peptone
water

IDENTIFICATION
• APPROPRIATE BIOCHEMICAL TESTS
- EG- INDOLE, TSI, CITRATE UTILISATION, UREA HYDROLYSIS,
METHYL RED, VP
.
TSI
Test

SEROTYPING
– BY GROUP SPECIFIC AND TYPE SPECIFIC ANTISERA
ANTIMICROBIAL SUCEPTIBILITY TESTING
– FOR CHOOSING APPROPRIATE DRUG FOR TREATMENT

TISSUE CULTURE-
• FOR DETECTION OF ENTERO VIRUS
• ETEC, EHEC
ANTIGEN DETECTION-
• ELISA
• ICT
• LATEX AGGLUTINATION TEST
MOLECULAR METHODS-
• PCR
TOXIC DETECTION-
• ELISA
• PCR

LAB DIAGNOSIS OF NON DIARRHOEAL
DISEASE
• For typhoid : Blood –Culture and sensitivity & widal test
• For hepatitis: serum- ELISA, vidas , immunochromatographic test.

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