Ques-1: Chlamydia trichomatis has small G shaped intracellular bacteria with noninfectious reticulate form and infectious elementary body. It is most sexually transmitting disease in world. An endocervical swab was obtained from the patient & used to inoculate McCoy cells, after 48 hours of incubation, the McCoy cells were stained with an imunnofluorescent reagent that demonstrated the presence of inclusions. All Chlamydia are anaerobically performing their metabolism using fermentation of various body fluids including vaginal fluids and they perform biphasic life cycle with both infectious replication & non-replicative phases in McCoy cells. Chlamydia bacteria are ovoid in shape and they are non-motile. They do not produce spores but possess infectious elementary bodies to release into the host cells. Ques-2: Sonographic bimanual examination in the pelvic examination & nucleic acid amplification test (NAAT) are useful to methodologies currently exist to identify Chlamydia trachomatis. The results are compared with tissue culture sensitivity based on the growth level on the medium Ques-3: Beta-lactam antibiotics in treating infections caused by this organism: Beta -lactum antibiotics such as penicillins, sulfonamides contain beta-lactum ring in their structures, examples include penicillin, cephalosporins, carbapenems and monobactum antibiotics. They act in inhibiting the cell wall synthesis, therefore they considered as bactericidal. These antibiotics are generally effective against Gram positive bacteria and some are also effective against gram negative bacteria. Examples include staphylococcus species, streptococcus species. Doxycycline is going to inhibit protein synthesis of \"this microbial species\" because beta-lactum are essential to act \"against drug resistance properties of microbes\". The other epidemiological studies are needed to perform endocervical swab test to determine any presence of Neisseria gonorrhoeae that cause PID These penicillins are active against the gram- positive bacteria and they act by inhibiting the cell wall synthesis. Transition state analog of penicillin & enzyme: Chemical reaction of penicillin (beta-lactum ring) in reaction with N-C bond of D-ala-D-ala analog i.e. glycoproteinpeptidase finally result in formation of \"transition state analog\". The mechanism is mainly mediated by suicide inhibition of the enzyme irreversibly by reacting with enzyme Solution Ques-1: Chlamydia trichomatis has small G shaped intracellular bacteria with noninfectious reticulate form and infectious elementary body. It is most sexually transmitting disease in world. An endocervical swab was obtained from the patient & used to inoculate McCoy cells, after 48 hours of incubation, the McCoy cells were stained with an imunnofluorescent reagent that demonstrated the presence of inclusions. All Chlamydia are anaerobically performing their metabolism using fermentation of various body fluids including vaginal fluids and they perform.

1. Ques-1:
Chlamydia trichomatis has small G shaped intracellular bacteria with noninfectious reticulate
form and infectious elementary body. It is most sexually transmitting disease in world.
An endocervical swab was obtained from the patient & used to inoculate McCoy cells, after 48
hours of incubation, the McCoy cells were stained with an imunnofluorescent reagent that
demonstrated the presence of inclusions. All Chlamydia are anaerobically performing their
metabolism using fermentation of various body fluids including vaginal fluids and they perform
biphasic life cycle with both infectious replication & non-replicative phases in McCoy cells.
Chlamydia bacteria are ovoid in shape and they are non-motile. They do not produce spores but
possess infectious elementary bodies to release into the host cells.
Ques-2:
Sonographic bimanual examination in the pelvic examination & nucleic acid amplification test
(NAAT) are useful to methodologies currently exist to identify Chlamydia trachomatis. The
results are compared with tissue culture sensitivity based on the growth level on the medium
Ques-3:
Beta-lactam antibiotics in treating infections caused by this organism:
Beta -lactum antibiotics such as penicillins, sulfonamides contain beta-lactum ring in their
structures, examples include penicillin, cephalosporins, carbapenems and monobactum
antibiotics. They act in inhibiting the cell wall synthesis, therefore they considered as
bactericidal. These antibiotics are generally effective against Gram positive bacteria and some
are also effective against gram negative bacteria. Examples include staphylococcus species,
streptococcus species. Doxycycline is going to inhibit protein synthesis of "this microbial
species" because beta-lactum are essential to act "against drug resistance properties of
microbes". The other epidemiological studies are needed to perform endocervical swab test to
determine any presence of Neisseria gonorrhoeae that cause PID
These penicillins are active against the gram- positive bacteria and they act by inhibiting the cell
wall synthesis. Transition state analog of penicillin & enzyme: Chemical reaction of penicillin
(beta-lactum ring) in reaction with N-C bond of D-ala-D-ala analog i.e. glycoproteinpeptidase
finally result in formation of "transition state analog". The mechanism is mainly mediated by
suicide inhibition of the enzyme irreversibly by reacting with enzyme
Solution
Ques-1:

2. Chlamydia trichomatis has small G shaped intracellular bacteria with noninfectious reticulate
form and infectious elementary body. It is most sexually transmitting disease in world.
An endocervical swab was obtained from the patient & used to inoculate McCoy cells, after 48
hours of incubation, the McCoy cells were stained with an imunnofluorescent reagent that
demonstrated the presence of inclusions. All Chlamydia are anaerobically performing their
metabolism using fermentation of various body fluids including vaginal fluids and they perform
biphasic life cycle with both infectious replication & non-replicative phases in McCoy cells.
Chlamydia bacteria are ovoid in shape and they are non-motile. They do not produce spores but
possess infectious elementary bodies to release into the host cells.
Ques-2:
Sonographic bimanual examination in the pelvic examination & nucleic acid amplification test
(NAAT) are useful to methodologies currently exist to identify Chlamydia trachomatis. The
results are compared with tissue culture sensitivity based on the growth level on the medium
Ques-3:
Beta-lactam antibiotics in treating infections caused by this organism:
Beta -lactum antibiotics such as penicillins, sulfonamides contain beta-lactum ring in their
structures, examples include penicillin, cephalosporins, carbapenems and monobactum
antibiotics. They act in inhibiting the cell wall synthesis, therefore they considered as
bactericidal. These antibiotics are generally effective against Gram positive bacteria and some
are also effective against gram negative bacteria. Examples include staphylococcus species,
streptococcus species. Doxycycline is going to inhibit protein synthesis of "this microbial
species" because beta-lactum are essential to act "against drug resistance properties of
microbes". The other epidemiological studies are needed to perform endocervical swab test to
determine any presence of Neisseria gonorrhoeae that cause PID
These penicillins are active against the gram- positive bacteria and they act by inhibiting the cell
wall synthesis. Transition state analog of penicillin & enzyme: Chemical reaction of penicillin
(beta-lactum ring) in reaction with N-C bond of D-ala-D-ala analog i.e. glycoproteinpeptidase
finally result in formation of "transition state analog". The mechanism is mainly mediated by
suicide inhibition of the enzyme irreversibly by reacting with enzyme