What are four differences between transcription and replication What are four differences between transcription and replication Solution Ans : The difference between transcription and replication are : 1) In transcription , small portion of DNA is used , whereas in replication large portion (whole) of DNA is used. 2) No primer is required in transcription , whereas it is required in treplication. 3) DNA polymerase enzyme is used in replication and RNA polymerase enzyme is used in trasnscription. 4) No proofreading is done in transcription whereas it is done in replication. .