Zdravljenje vbodne rane na kopitu z ličinkami muh Nina Bas
8- letni žrebec je bil prvi pacient v slovenski veterini, ki je bil zdravljen z ličinkami muh.
Primer sem predstavila na 4. veterinarskem kongresu v Portorožu, novembra 2011.
Prispevek je avtorski, vsako kopiranje in razmnoževanje brez avtoričinega dovoljenja je prepovedano.
Photographie Lot 1601- 1920
Auktion: Samstag, 3. Dezember 2016, 10.00 Uhr Vorbesichtigung: Fr. 25. bis Di. 29. November 2016
Gabriel Müller, M.A. Tel. +41 44 445 63 40 mueller@kollerauktionen.ch Historische - und Reisephotographie 1601-1632
Helvetica 1633-1668
Klassische Photographie 1669-1736
Portraits 1737-1827
Varia 1828-1851
Sammlung Michel Comte 1852-1886
Zeitgenössische Photographie 1887-1920
Este artículo presenta el estudio de un depósito arqueológico en el corregimiento de Santa Rita. Andes. Antioquia (2001), aportando información sobre los procesos de formación de sitio a nivel natural y cultural que remiten a las prácticas agrícolas y el manejo de ecosistemas, además se identifica una secuencia cultural que permite sugerir grandes fases de ocupación que reflejan procesos de cambio cultural y apropiación del territorio.
Zdravljenje vbodne rane na kopitu z ličinkami muh Nina Bas
8- letni žrebec je bil prvi pacient v slovenski veterini, ki je bil zdravljen z ličinkami muh.
Primer sem predstavila na 4. veterinarskem kongresu v Portorožu, novembra 2011.
Prispevek je avtorski, vsako kopiranje in razmnoževanje brez avtoričinega dovoljenja je prepovedano.
Photographie Lot 1601- 1920
Auktion: Samstag, 3. Dezember 2016, 10.00 Uhr Vorbesichtigung: Fr. 25. bis Di. 29. November 2016
Gabriel Müller, M.A. Tel. +41 44 445 63 40 mueller@kollerauktionen.ch Historische - und Reisephotographie 1601-1632
Helvetica 1633-1668
Klassische Photographie 1669-1736
Portraits 1737-1827
Varia 1828-1851
Sammlung Michel Comte 1852-1886
Zeitgenössische Photographie 1887-1920
Este artículo presenta el estudio de un depósito arqueológico en el corregimiento de Santa Rita. Andes. Antioquia (2001), aportando información sobre los procesos de formación de sitio a nivel natural y cultural que remiten a las prácticas agrícolas y el manejo de ecosistemas, además se identifica una secuencia cultural que permite sugerir grandes fases de ocupación que reflejan procesos de cambio cultural y apropiación del territorio.
2024 State of Marketing Report – by HubspotMarius Sescu
https://www.hubspot.com/state-of-marketing
· Scaling relationships and proving ROI
· Social media is the place for search, sales, and service
· Authentic influencer partnerships fuel brand growth
· The strongest connections happen via call, click, chat, and camera.
· Time saved with AI leads to more creative work
· Seeking: A single source of truth
· TLDR; Get on social, try AI, and align your systems.
· More human marketing, powered by robots
ChatGPT is a revolutionary addition to the world since its introduction in 2022. A big shift in the sector of information gathering and processing happened because of this chatbot. What is the story of ChatGPT? How is the bot responding to prompts and generating contents? Swipe through these slides prepared by Expeed Software, a web development company regarding the development and technical intricacies of ChatGPT!
Product Design Trends in 2024 | Teenage EngineeringsPixeldarts
The realm of product design is a constantly changing environment where technology and style intersect. Every year introduces fresh challenges and exciting trends that mold the future of this captivating art form. In this piece, we delve into the significant trends set to influence the look and functionality of product design in the year 2024.
How Race, Age and Gender Shape Attitudes Towards Mental HealthThinkNow
Mental health has been in the news quite a bit lately. Dozens of U.S. states are currently suing Meta for contributing to the youth mental health crisis by inserting addictive features into their products, while the U.S. Surgeon General is touring the nation to bring awareness to the growing epidemic of loneliness and isolation. The country has endured periods of low national morale, such as in the 1970s when high inflation and the energy crisis worsened public sentiment following the Vietnam War. The current mood, however, feels different. Gallup recently reported that national mental health is at an all-time low, with few bright spots to lift spirits.
To better understand how Americans are feeling and their attitudes towards mental health in general, ThinkNow conducted a nationally representative quantitative survey of 1,500 respondents and found some interesting differences among ethnic, age and gender groups.
Technology
For example, 52% agree that technology and social media have a negative impact on mental health, but when broken out by race, 61% of Whites felt technology had a negative effect, and only 48% of Hispanics thought it did.
While technology has helped us keep in touch with friends and family in faraway places, it appears to have degraded our ability to connect in person. Staying connected online is a double-edged sword since the same news feed that brings us pictures of the grandkids and fluffy kittens also feeds us news about the wars in Israel and Ukraine, the dysfunction in Washington, the latest mass shooting and the climate crisis.
Hispanics may have a built-in defense against the isolation technology breeds, owing to their large, multigenerational households, strong social support systems, and tendency to use social media to stay connected with relatives abroad.
Age and Gender
When asked how individuals rate their mental health, men rate it higher than women by 11 percentage points, and Baby Boomers rank it highest at 83%, saying it’s good or excellent vs. 57% of Gen Z saying the same.
Gen Z spends the most amount of time on social media, so the notion that social media negatively affects mental health appears to be correlated. Unfortunately, Gen Z is also the generation that’s least comfortable discussing mental health concerns with healthcare professionals. Only 40% of them state they’re comfortable discussing their issues with a professional compared to 60% of Millennials and 65% of Boomers.
Race Affects Attitudes
As seen in previous research conducted by ThinkNow, Asian Americans lag other groups when it comes to awareness of mental health issues. Twenty-four percent of Asian Americans believe that having a mental health issue is a sign of weakness compared to the 16% average for all groups. Asians are also considerably less likely to be aware of mental health services in their communities (42% vs. 55%) and most likely to seek out information on social media (51% vs. 35%).
AI Trends in Creative Operations 2024 by Artwork Flow.pdfmarketingartwork
This article is all about what AI trends will emerge in the field of creative operations in 2024. All the marketers and brand builders should be aware of these trends for their further use and save themselves some time!
A report by thenetworkone and Kurio.
The contributing experts and agencies are (in an alphabetical order): Sylwia Rytel, Social Media Supervisor, 180heartbeats + JUNG v MATT (PL), Sharlene Jenner, Vice President - Director of Engagement Strategy, Abelson Taylor (USA), Alex Casanovas, Digital Director, Atrevia (ES), Dora Beilin, Senior Social Strategist, Barrett Hoffher (USA), Min Seo, Campaign Director, Brand New Agency (KR), Deshé M. Gully, Associate Strategist, Day One Agency (USA), Francesca Trevisan, Strategist, Different (IT), Trevor Crossman, CX and Digital Transformation Director; Olivia Hussey, Strategic Planner; Simi Srinarula, Social Media Manager, The Hallway (AUS), James Hebbert, Managing Director, Hylink (CN / UK), Mundy Álvarez, Planning Director; Pedro Rojas, Social Media Manager; Pancho González, CCO, Inbrax (CH), Oana Oprea, Head of Digital Planning, Jam Session Agency (RO), Amy Bottrill, Social Account Director, Launch (UK), Gaby Arriaga, Founder, Leonardo1452 (MX), Shantesh S Row, Creative Director, Liwa (UAE), Rajesh Mehta, Chief Strategy Officer; Dhruv Gaur, Digital Planning Lead; Leonie Mergulhao, Account Supervisor - Social Media & PR, Medulla (IN), Aurelija Plioplytė, Head of Digital & Social, Not Perfect (LI), Daiana Khaidargaliyeva, Account Manager, Osaka Labs (UK / USA), Stefanie Söhnchen, Vice President Digital, PIABO Communications (DE), Elisabeth Winiartati, Managing Consultant, Head of Global Integrated Communications; Lydia Aprina, Account Manager, Integrated Marketing and Communications; Nita Prabowo, Account Manager, Integrated Marketing and Communications; Okhi, Web Developer, PNTR Group (ID), Kei Obusan, Insights Director; Daffi Ranandi, Insights Manager, Radarr (SG), Gautam Reghunath, Co-founder & CEO, Talented (IN), Donagh Humphreys, Head of Social and Digital Innovation, THINKHOUSE (IRE), Sarah Yim, Strategy Director, Zulu Alpha Kilo (CA).
Trends In Paid Search: Navigating The Digital Landscape In 2024Search Engine Journal
The search marketing landscape is evolving rapidly with new technologies, and professionals, like you, rely on innovative paid search strategies to meet changing demands.
It’s important that you’re ready to implement new strategies in 2024.
Check this out and learn the top trends in paid search advertising that are expected to gain traction, so you can drive higher ROI more efficiently in 2024.
You’ll learn:
- The latest trends in AI and automation, and what this means for an evolving paid search ecosystem.
- New developments in privacy and data regulation.
- Emerging ad formats that are expected to make an impact next year.
Watch Sreekant Lanka from iQuanti and Irina Klein from OneMain Financial as they dive into the future of paid search and explore the trends, strategies, and technologies that will shape the search marketing landscape.
If you’re looking to assess your paid search strategy and design an industry-aligned plan for 2024, then this webinar is for you.
5 Public speaking tips from TED - Visualized summarySpeakerHub
From their humble beginnings in 1984, TED has grown into the world’s most powerful amplifier for speakers and thought-leaders to share their ideas. They have over 2,400 filmed talks (not including the 30,000+ TEDx videos) freely available online, and have hosted over 17,500 events around the world.
With over one billion views in a year, it’s no wonder that so many speakers are looking to TED for ideas on how to share their message more effectively.
The article “5 Public-Speaking Tips TED Gives Its Speakers”, by Carmine Gallo for Forbes, gives speakers five practical ways to connect with their audience, and effectively share their ideas on stage.
Whether you are gearing up to get on a TED stage yourself, or just want to master the skills that so many of their speakers possess, these tips and quotes from Chris Anderson, the TED Talks Curator, will encourage you to make the most impactful impression on your audience.
See the full article and more summaries like this on SpeakerHub here: https://speakerhub.com/blog/5-presentation-tips-ted-gives-its-speakers
See the original article on Forbes here:
http://www.forbes.com/forbes/welcome/?toURL=http://www.forbes.com/sites/carminegallo/2016/05/06/5-public-speaking-tips-ted-gives-its-speakers/&refURL=&referrer=#5c07a8221d9b
ChatGPT and the Future of Work - Clark Boyd Clark Boyd
Everyone is in agreement that ChatGPT (and other generative AI tools) will shape the future of work. Yet there is little consensus on exactly how, when, and to what extent this technology will change our world.
Businesses that extract maximum value from ChatGPT will use it as a collaborative tool for everything from brainstorming to technical maintenance.
For individuals, now is the time to pinpoint the skills the future professional will need to thrive in the AI age.
Check out this presentation to understand what ChatGPT is, how it will shape the future of work, and how you can prepare to take advantage.
A brief introduction to DataScience with explaining of the concepts, algorithms, machine learning, supervised and unsupervised learning, clustering, statistics, data preprocessing, real-world applications etc.
It's part of a Data Science Corner Campaign where I will be discussing the fundamentals of DataScience, AIML, Statistics etc.
Time Management & Productivity - Best PracticesVit Horky
Here's my presentation on by proven best practices how to manage your work time effectively and how to improve your productivity. It includes practical tips and how to use tools such as Slack, Google Apps, Hubspot, Google Calendar, Gmail and others.
The six step guide to practical project managementMindGenius
The six step guide to practical project management
If you think managing projects is too difficult, think again.
We’ve stripped back project management processes to the
basics – to make it quicker and easier, without sacrificing
the vital ingredients for success.
“If you’re looking for some real-world guidance, then The Six Step Guide to Practical Project Management will help.”
Dr Andrew Makar, Tactical Project Management
2024 State of Marketing Report – by HubspotMarius Sescu
https://www.hubspot.com/state-of-marketing
· Scaling relationships and proving ROI
· Social media is the place for search, sales, and service
· Authentic influencer partnerships fuel brand growth
· The strongest connections happen via call, click, chat, and camera.
· Time saved with AI leads to more creative work
· Seeking: A single source of truth
· TLDR; Get on social, try AI, and align your systems.
· More human marketing, powered by robots
ChatGPT is a revolutionary addition to the world since its introduction in 2022. A big shift in the sector of information gathering and processing happened because of this chatbot. What is the story of ChatGPT? How is the bot responding to prompts and generating contents? Swipe through these slides prepared by Expeed Software, a web development company regarding the development and technical intricacies of ChatGPT!
Product Design Trends in 2024 | Teenage EngineeringsPixeldarts
The realm of product design is a constantly changing environment where technology and style intersect. Every year introduces fresh challenges and exciting trends that mold the future of this captivating art form. In this piece, we delve into the significant trends set to influence the look and functionality of product design in the year 2024.
How Race, Age and Gender Shape Attitudes Towards Mental HealthThinkNow
Mental health has been in the news quite a bit lately. Dozens of U.S. states are currently suing Meta for contributing to the youth mental health crisis by inserting addictive features into their products, while the U.S. Surgeon General is touring the nation to bring awareness to the growing epidemic of loneliness and isolation. The country has endured periods of low national morale, such as in the 1970s when high inflation and the energy crisis worsened public sentiment following the Vietnam War. The current mood, however, feels different. Gallup recently reported that national mental health is at an all-time low, with few bright spots to lift spirits.
To better understand how Americans are feeling and their attitudes towards mental health in general, ThinkNow conducted a nationally representative quantitative survey of 1,500 respondents and found some interesting differences among ethnic, age and gender groups.
Technology
For example, 52% agree that technology and social media have a negative impact on mental health, but when broken out by race, 61% of Whites felt technology had a negative effect, and only 48% of Hispanics thought it did.
While technology has helped us keep in touch with friends and family in faraway places, it appears to have degraded our ability to connect in person. Staying connected online is a double-edged sword since the same news feed that brings us pictures of the grandkids and fluffy kittens also feeds us news about the wars in Israel and Ukraine, the dysfunction in Washington, the latest mass shooting and the climate crisis.
Hispanics may have a built-in defense against the isolation technology breeds, owing to their large, multigenerational households, strong social support systems, and tendency to use social media to stay connected with relatives abroad.
Age and Gender
When asked how individuals rate their mental health, men rate it higher than women by 11 percentage points, and Baby Boomers rank it highest at 83%, saying it’s good or excellent vs. 57% of Gen Z saying the same.
Gen Z spends the most amount of time on social media, so the notion that social media negatively affects mental health appears to be correlated. Unfortunately, Gen Z is also the generation that’s least comfortable discussing mental health concerns with healthcare professionals. Only 40% of them state they’re comfortable discussing their issues with a professional compared to 60% of Millennials and 65% of Boomers.
Race Affects Attitudes
As seen in previous research conducted by ThinkNow, Asian Americans lag other groups when it comes to awareness of mental health issues. Twenty-four percent of Asian Americans believe that having a mental health issue is a sign of weakness compared to the 16% average for all groups. Asians are also considerably less likely to be aware of mental health services in their communities (42% vs. 55%) and most likely to seek out information on social media (51% vs. 35%).
AI Trends in Creative Operations 2024 by Artwork Flow.pdfmarketingartwork
This article is all about what AI trends will emerge in the field of creative operations in 2024. All the marketers and brand builders should be aware of these trends for their further use and save themselves some time!
A report by thenetworkone and Kurio.
The contributing experts and agencies are (in an alphabetical order): Sylwia Rytel, Social Media Supervisor, 180heartbeats + JUNG v MATT (PL), Sharlene Jenner, Vice President - Director of Engagement Strategy, Abelson Taylor (USA), Alex Casanovas, Digital Director, Atrevia (ES), Dora Beilin, Senior Social Strategist, Barrett Hoffher (USA), Min Seo, Campaign Director, Brand New Agency (KR), Deshé M. Gully, Associate Strategist, Day One Agency (USA), Francesca Trevisan, Strategist, Different (IT), Trevor Crossman, CX and Digital Transformation Director; Olivia Hussey, Strategic Planner; Simi Srinarula, Social Media Manager, The Hallway (AUS), James Hebbert, Managing Director, Hylink (CN / UK), Mundy Álvarez, Planning Director; Pedro Rojas, Social Media Manager; Pancho González, CCO, Inbrax (CH), Oana Oprea, Head of Digital Planning, Jam Session Agency (RO), Amy Bottrill, Social Account Director, Launch (UK), Gaby Arriaga, Founder, Leonardo1452 (MX), Shantesh S Row, Creative Director, Liwa (UAE), Rajesh Mehta, Chief Strategy Officer; Dhruv Gaur, Digital Planning Lead; Leonie Mergulhao, Account Supervisor - Social Media & PR, Medulla (IN), Aurelija Plioplytė, Head of Digital & Social, Not Perfect (LI), Daiana Khaidargaliyeva, Account Manager, Osaka Labs (UK / USA), Stefanie Söhnchen, Vice President Digital, PIABO Communications (DE), Elisabeth Winiartati, Managing Consultant, Head of Global Integrated Communications; Lydia Aprina, Account Manager, Integrated Marketing and Communications; Nita Prabowo, Account Manager, Integrated Marketing and Communications; Okhi, Web Developer, PNTR Group (ID), Kei Obusan, Insights Director; Daffi Ranandi, Insights Manager, Radarr (SG), Gautam Reghunath, Co-founder & CEO, Talented (IN), Donagh Humphreys, Head of Social and Digital Innovation, THINKHOUSE (IRE), Sarah Yim, Strategy Director, Zulu Alpha Kilo (CA).
Trends In Paid Search: Navigating The Digital Landscape In 2024Search Engine Journal
The search marketing landscape is evolving rapidly with new technologies, and professionals, like you, rely on innovative paid search strategies to meet changing demands.
It’s important that you’re ready to implement new strategies in 2024.
Check this out and learn the top trends in paid search advertising that are expected to gain traction, so you can drive higher ROI more efficiently in 2024.
You’ll learn:
- The latest trends in AI and automation, and what this means for an evolving paid search ecosystem.
- New developments in privacy and data regulation.
- Emerging ad formats that are expected to make an impact next year.
Watch Sreekant Lanka from iQuanti and Irina Klein from OneMain Financial as they dive into the future of paid search and explore the trends, strategies, and technologies that will shape the search marketing landscape.
If you’re looking to assess your paid search strategy and design an industry-aligned plan for 2024, then this webinar is for you.
5 Public speaking tips from TED - Visualized summarySpeakerHub
From their humble beginnings in 1984, TED has grown into the world’s most powerful amplifier for speakers and thought-leaders to share their ideas. They have over 2,400 filmed talks (not including the 30,000+ TEDx videos) freely available online, and have hosted over 17,500 events around the world.
With over one billion views in a year, it’s no wonder that so many speakers are looking to TED for ideas on how to share their message more effectively.
The article “5 Public-Speaking Tips TED Gives Its Speakers”, by Carmine Gallo for Forbes, gives speakers five practical ways to connect with their audience, and effectively share their ideas on stage.
Whether you are gearing up to get on a TED stage yourself, or just want to master the skills that so many of their speakers possess, these tips and quotes from Chris Anderson, the TED Talks Curator, will encourage you to make the most impactful impression on your audience.
See the full article and more summaries like this on SpeakerHub here: https://speakerhub.com/blog/5-presentation-tips-ted-gives-its-speakers
See the original article on Forbes here:
http://www.forbes.com/forbes/welcome/?toURL=http://www.forbes.com/sites/carminegallo/2016/05/06/5-public-speaking-tips-ted-gives-its-speakers/&refURL=&referrer=#5c07a8221d9b
ChatGPT and the Future of Work - Clark Boyd Clark Boyd
Everyone is in agreement that ChatGPT (and other generative AI tools) will shape the future of work. Yet there is little consensus on exactly how, when, and to what extent this technology will change our world.
Businesses that extract maximum value from ChatGPT will use it as a collaborative tool for everything from brainstorming to technical maintenance.
For individuals, now is the time to pinpoint the skills the future professional will need to thrive in the AI age.
Check out this presentation to understand what ChatGPT is, how it will shape the future of work, and how you can prepare to take advantage.
A brief introduction to DataScience with explaining of the concepts, algorithms, machine learning, supervised and unsupervised learning, clustering, statistics, data preprocessing, real-world applications etc.
It's part of a Data Science Corner Campaign where I will be discussing the fundamentals of DataScience, AIML, Statistics etc.
Time Management & Productivity - Best PracticesVit Horky
Here's my presentation on by proven best practices how to manage your work time effectively and how to improve your productivity. It includes practical tips and how to use tools such as Slack, Google Apps, Hubspot, Google Calendar, Gmail and others.
The six step guide to practical project managementMindGenius
The six step guide to practical project management
If you think managing projects is too difficult, think again.
We’ve stripped back project management processes to the
basics – to make it quicker and easier, without sacrificing
the vital ingredients for success.
“If you’re looking for some real-world guidance, then The Six Step Guide to Practical Project Management will help.”
Dr Andrew Makar, Tactical Project Management
Beginners Guide to TikTok for Search - Rachel Pearson - We are Tilt __ Bright...
Univerza v Ljubljani, Biotehniška fakulteta, Študij
1. DIPLOMSKI SEMINAR
PROIZVODNJA TERAPEVTSKIHPROIZVODNJA TERAPEVTSKIH
PROTEINOV V MLEKUPROTEINOV V MLEKU
(gene pharming)(gene pharming)
Avtorica: Urška DOLINAR Mentor: Peter DOVČAvtorica: Urška DOLINAR Mentor: Peter DOVČ
2. “Gene pharming” predstavlja proizvodnjo terapevtskih
proteinov v transgenih živalih kot so: govedo, ovce, koze,
svinje in kunci.
Terapevtski proteini imajo visoko biomedicinsko uporabnost v
zdravljenju bolezni in vključujejo: hormone, rastne faktorje,
protitelesa, cepiva in faktorje strjevanja krvi.
3. 1987 - Proteini, ki običajno niso prisotni v mleku, se lahko
izražajo v mlečni žlezi transgenih miši- tPA
1989 - prvo transgeno tele Herman z genom za človeški
laktoferin.
1990 - Izražanje IL-2 v mleku transgenih kuncev.
1991 - Ovca Tracy z genom za človeški α-1-antitripsin
1997- Krava Rossie izloča človeški α- laktalbumin v mleko
4. MLEKO je najboljši bioreaktor :
ni nevarnosti prenosa virusnih okužb (HIV, Hep.C)
čist, enostaven in higieničen produkt
čiščenje in izolacija poceni in nezahtevna
enostavno zbiranje in dostopnost v velikih količinah
količina izločenih proteinov večja kot v ostalih sistemih
večje količine proteinov nimajo vpliva na zdravje živali
MLEČNA ŽLEZA je najboljše produkcijsko tkivo:
visoka učinkovitost sinteze
velika količina izraženih proteinov
nižja cena sinteze v primerjavi s celičnimi kulturami
5. Z njim dosežemo izražanje tujega proteina specifično v mlečni žlezi
transgene živali.
Sestavljen iz:
tkivno specifičnega promotorja za mlečni proteintkivno specifičnega promotorja za mlečni protein (delujejo medvrstno)
kodirajoče sekvence želenega proteina
6. ODVISNA OD:
časa do 1. laktacije
količine mleka
stopnje ekspresije
sekrecije proteinov
izvedljivosti in cene gojenja
KunciKunci: najmanjši,najlažje vzdrževani
GovedoGovedo: najdražja, najdaljši čas vzreje
Ovce in kozeOvce in koze: idealne za proizvodnjo večjih količin mleka, nezahtevne za rejo.
Lahko proizvedejo do 30g/L proteina, 100L letno = 3kg terapevtske
učinkovine.
8. Podrazred retrovirusov
Odstranimo gene za replikacijo in
jih zamenjamo z želenim
transgenom → virus po vgraditvi v
genom ni infektiven.
Za proizvodnjo 3 komponente:
vektorski konstrukt, konstruktvektorski konstrukt, konstrukt
pakiranja in konstrukt ovojnice.pakiranja in konstrukt ovojnice.
Plazmide vstavimo v celice
odgovorne za pakiranje, ki
producirajo lentiviruse.
9. Prednosti metode:Prednosti metode:
velik genom in zmožnost prenosa večjih konstruktov,
transfekcija več zarodkov hkrati,
širok spekter tarčnih gostiteljskih celic,
prenos DNA v občutljive celice in celice, ki se ne delijo
4X večja učinkovitost od mikroinjiciranja
mestno specifična in stabilna integracija ene kopije transgena.
Slabosti metode:Slabosti metode:
dolgotrajnost priprave velikih količin rekombinantnih lentivirusov,
lahko povzročajo infekcijske bolezni, če niso pravilno oslabljeni,
potrebna uporaba laboratorija 3. varnostne stopnje.
10. Proteini, proizvedeni v mlečni žlezi transgenih živali morajo biti: enostavni
za identifikacijo, čisti, učinkoviti, skladni s predpisi in varni za uporabo.
Specifičnost čiščenja je odvisna od proteina in sprejemljivih stopenj čistosti,
ki so določene z namenom uporabe.
11. Več kot 130 proteinov je odobrenih za klinično uporabo s strani FDA, veliko
več jih je v razvoju.
FAKTORJI STRJEVANJA KRVIFAKTORJI STRJEVANJA KRVI (hFVII, hFVIII, hFIX)(hFVII, hFVIII, hFIX)
uporaba v zdravljenju hemofilije A, B in C
v začetku izolirani iz človeške krvne plazme
danes proizvedeni v transgenih živalih
ANTIKOAGULANTIANTIKOAGULANTI (hPC, hAT-III, htPA)(hPC, hAT-III, htPA)
preprečujejo strjevanje krvi.
uporabni pri zdravljenju tromboze, pljučnega embolizma, miokardnega
infarkta, kapi, sepse, ledvičnih in jetrnih bolezni.
12. RASTNI FAKTORJI IN HORMONIRASTNI FAKTORJI IN HORMONI (hGH, hEPO)(hGH, hEPO)
stimulirajo celično rast, proliferacijo in diferenciacijo.
običajno proteini ali steroidni hormoni.
hematopoetski proteini omogočajo zorenje krvnih celic. Uporabljamo jih
pri zdravljenju anemij, ki so posledica kronične ledvične odpovedi,
presaditve organov ali kemoterapije pri zdravljenju raka.
PLAZEMSKI IN PROTIVNETNI PROTEINIPLAZEMSKI IN PROTIVNETNI PROTEINI (hSA, hLF, hα1AT)(hSA, hLF, hα1AT)
Imajo antibakterijsko, antivirusno, antiparazitsko, protitumorsko
antialergijsko, antioksidativno in imunomodulativno učinkovitost.
Lahko se uporabljajo kot prehransko dopolnilo.
hSAhSA ohranja osmotski tlak in obnavlja kroženje volumna krvi. Uporablja
se v terapiji šoka, hudih opeklin in nezgodni kirurgiji.
Alfa-1- antitripsinAlfa-1- antitripsin deluje protivnetno, zdravi pljučne bolezni in ciroze,
emifizme in cistično fibrozo.
13. Pred administracijo mora biti potrjena njihova varnost in učinkovitost.
Predklinična testiranjaPredklinična testiranja: preverjanje biokemijskih, toksičnih in
farmakinetičnih lastnosti. Zbiranje podrobnih informacij o viru in namenu
proizvodnje.
Klinični testiKlinični testi :
Faza IFaza I: testiranje na stranske učinke zdravila na majhnem številu zdravih
ljudi.
Faza IIFaza II: pacienti in kontrolne skupine za nadaljnje vrednotenje varnosti in
učinkovitosti.
Faza IIIFaza III: vrednotenje na veliko večjih skupinah pacientov in kontrol ter
določitev predlagane uporabe in doziranja.
Sledi odobritev za klinično uporabo.odobritev za klinično uporabo.
Faza IVFaza IV: določanje vpliva dolgoročnega apliciranja na paciente.
14. Prvi transgeni produkt, odobren za terapevtsko uporabo je antitrombin IIIantitrombin III
(2006, GTC Biotherapeutics).(2006, GTC Biotherapeutics). Zaključil je III. fazo kliničnega preizkušanja v
ZDA .
Drugi je produkt PPL therapeutics, α-1- antitripsin. Prešel je II. fazo kliničnih
testiranj pri pacientih s cistično fibrozno (vendar še ni bil potrjen s strani
FDA).
Za proizvodnjo v smeri »pharminga« niso razlog le manjši stroški, ampak tudi
dostopnost zdravila v zadostni količini.
15. HVALA ZA VAŠO POZORNOSTHVALA ZA VAŠO POZORNOST
Iskrena hvala g. mentorju prof. dr. Petru Dovču, ki me je usmerjal
med pisanjem diplomskega seminarja.
Hvaležna sem družini in fantu za nesebično podporo, prijateljem, ki
so me tolažili in me nasmejali, ko ni šlo vse kot po maslu.
Iz srca se zahvaljujem Vsem,
ki ste me spremljali na poti mojega študija.