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Principles of
Biological Science I Laboratory
           BSC110L


      Kristen Covino
       covinok@gmail.com
            JST 810
Science
                 A Way of Knowing

• Systematic knowledge of the world gained through
  observation and experimentation


• Systematic enterprise of gathering knowledge about
  the world and organizing that knowledge into testable
  laws and theories

• Any systematic knowledge that is capable of resulting
  in a correct prediction or reliable outcome

• Exercise 1-1
Biology

Living

         Study of


Study of Life
Epistemological Cycle
                     Study of Understanding
                        Scientific Method
                           hypothesis
                                              prediction



                                  question
conclusion
(accept or reject)
                                                experiment
                           observation          (gathering data)




                              results
Scientific Method

1. Observation

   Male Red-winged Blackbirds have red wing epaulet,
   expose epaulet when defending territory

          Question: Is the red epaulet exposed as a signal or threat
          to other males?




                            Red-winged Blackbird
                            (Agelaius phoeniceus )




        Male                                                Female
Scientific Method

 hypothesis
                  Hypothesis – possible explanation for the observed facts;
                                  leads to a testable PREDICTION
       question


 observation




                   Red Epaulet as a Threat Signal
Hypothesis: Males have red wing epaulet as a signal of threat to other males

         Prediction: Males without a red epaulet will not be considered
                            a threat to other males
Hypothesis: Males have red wing epaulet as a signal of threat to other males

Prediction: Males without a red epaulet will not be considered
             a threat to other males

                             Experiment
                        Hansen and Rohwer (1986)

  • Manipulated the size of epaulet (double, half, black, normal)

  • Placed dummy bird in another bird’s territory

  • Counted the number of times the territorial male hit the dummy
    bird (hitting dummy bird, signaled the dummy bird was a threat)




    Double                Half               Normal                 Black
hypothesis
                                                   Scientific Method
                prediction


     question

                  experiment
observation       (gathering data)

                                         Experiment – designed to test the prediction

1. Define Variables

   independent variable - variable that is manipulated/changed in the experiment

   dependent variable –              variable being measured, counted, or observed;
                                      changes according to the level of the other variable

   control variables –               variables kept constant during the experiment


   2. Outline a Procedure

         Sequence of steps for the experiment
         (can be designed from published work, collaborations, novel ideas)

        Replication of the procedure

        Include a Control – independent variable is held at an established level or omitted
Hypothesis: Males have red wing epaulet as a signal of threat to other males

Prediction: Males without a red epaulet will not be considered
             a threat to other males

                             Experiment
                        Hansen and Rohwer (1986)

  • Manipulated the size of epaulet (double, half, black, normal)

  • Placed dummy bird in another bird’s territory

  • Counted the number of times the territorial male hit the dummy
    bird (hitting dummy bird, signaled the dummy bird was a threat)




    Double                Half               Normal                 Black
Types of Observation /Description
• Good science depends very heavily on careful
  observations and accurate descriptions.

• Qualitative vs. quantitative descriptions
   – Qualitative – can be biased, subjective, influenced by
     opinion or prior experience (examples – color, smell,
     general size description such as “big”)
   – Quantitative – can be verified by anyone, objective,
     nothing left to judgment (example – exact size
     measurements, weight, facts about distinguishing
     marks)

• Exercise 1-2
hypothesis
                prediction
                                  Scientific Method
     question

                  experiment
observation       (gathering
                  data)
                               Results – organization and summary of data collected

  results                        - Present data collected in tables and figures
                                    - help in analyzing and interpreting your results
                                    - help a reader or viewer understand your results
                                    - no overlap of information among table and figures

                                 - Summary of data should be written precisely and
                                   accurately, NO fluff

 Tables and Figures
     Data Points
     Title
     Figure or Table # (with a caption describing content)
     Y-axis & X-axis labeled (include units if appropriate)
     Legend (if appropriate)
Results – Red-winged Blackbird Experiment



                                                                                                           Response to Wing Epaulet Size
                                                                                                     8



                                                                                                     6




                                                                Number of Hits
                                                                                 (per ten minutes)
Table 1.1 Number of hits on mounted red-winged blackbird

by territory owner in relation to the size of the red epaulet
                                                                                                     4
of the mount

     Size of Epaulet                    Number of Hits
                                                                                                     2
           Double                                 7

           Normal                                 4

               Half                              2.2                                                 0
                                                                                                                Double     Normal       Half      Black
            Black                                1.8


                                                                                                                      Wing Epaulet Size
                                                                                                         Figure 1. Number of hits on mounted red-winged
                                                                                                         blackbird by territory owners in relation to the size
                                                                                                         of the red epaulet of the mount.
hypothesis
                                     prediction
                                                      Scientific Method
                          question
conclusion                             experiment
(accept of reject)
                     observation       (gathering
                                       data)
                                                    Conclusion – explanation of results,
                                                                   accept or reject hypothesis,
                                                                   discuss future research
                       results




     We do NOT prove a hypothesis, we state that the results
     support our hypothesis

     Rejecting the hypothesis is just as important as finding
     support for a hypothesis
Conclusion


               Red-winged Blackbird Conclusion

    Males without red epaulet are considered less of a threat to
          territorial males than males with a red epaulet

Suggests that the red epaulet is used by males as a signal of threat




                           Red-winged Blackbird
                           (Agelaius phoeniceus )
Topic 13
                    (pages 127-129)




How to Write a Scientific Report
What is the point of a   Parts of a scientific
    scientific paper?       paper:
• Communicate information
  on your experiment in a    Abstract
  clear and concise way      Introduction
                             Materials and Methods
• Present results to         Results
  scientific community
                             Discussion
  and/or peers
                             Literature Cited
Abstract
• A summary of the experiment and its results
   – 5-7 sentences (2-3 background, 1-2 methods, 2-3
     results, 1-2 conclusions)

Introduction (2-4 WELL WRITTEN paragraphs)
• Background information

• Goal of your experiment
• State Hypothesis

• *Cite literature*
Materials and Methods
 • Describe what you did and the materials that you
   used so someone else could replicate the
   experiment
 • Paragraph form!! No lists!!
 • Omit unnecessary information
    – i.e. “We labeled the tubes, “tube 1”, “tube 2”…”
           “We made graphs using Excel…”

 Results
• General trends in data, NO RAW DATA!
• Don’t explain data, just present in paragraph form
• Represent data in tables and graphs
Graphs
• Labels: Title, x-axis, y-axis
• Key (optional and depends on the type of data)
• Legend (always, Always, ALWAYS!!)

                          One variable graph




               Figure 1: The number of cars parked in the USM 4 th street
Exercise 1-4   parking lot on 24 August, 2011 separated by color.
Graphs
• Labels: Title, x-axis, y-axis
• Key (optional and depends on the type of data)
• Legend (always, Always, ALWAYS!!)

                           One variable graph




               Figure 2: The number of cars parked in the USM 4 th street parking
Exercise 1-4   lot on 24 August, and 25 August 2011 separated by color.
Graphs
• Labels: Title, x-axis, y-axis
• Key (optional and depends on the type of graph)
• Legend (always, Always, ALWAYS!!)

                         Two variable graph




Exercise 1-4
               Figure 3: Mass of 10 books on my bookshelf compared to
               the number of pages in each book.
Discussion
• 2-4 well written paragraphs
• What do you think your results mean?
• Did you support or reject your hypothesis?
  – You cannot prove a hypothesis, if you say this, you
    will make me angry!
• Future questions?
• *Cite literature*
Literature Cited
• In the Literature Cited section:
Author, Date. Title. Journal. Volume: Pages.

Ex) Smith, A., 1995. Hogsucker diet survey. Fish
  Food. 18: 25-30.

• In the text (Introduction/Discussion):
The diet of Hypentilium etowanum, the Alabama
  hogsucker, consists mainly of insect larvae and small
  crustaceans (Smith, 1995).

Smith (1995) found that the diet of the Alabama hogsucker
  consists mainly of insect larvae and small crustaceans.
Literature Cited
• You must cite at least 3 references from
  scientific journals in your lab report
http://www.lib.usm.edu/find_items/article_database
  s.html

Other hints:
•   Use the rubric to guide you
•   Length is not as important as QUALITY
•   Grammar and spell-check!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
•   Have someone else read it, or read it aloud
•   Make it look professional: 10/12pt, normal font choice
•   PUT YOUR NAME ON IT!!!
Today/this week...
• Sign Safety Awareness Agreement
• Sign e-mail list/”seating” chart

• Handouts: Topic 1 supplement & guide to writing scientific
  papers

• Complete Exercises 1-1 , 1-2, & 1-3
• TURN IN lab manual pages 11 – 14

• Online plagiarism tutorial (due by Friday 5pm)
http://www.lib.usm.edu/legacy/plag/plagiarismtutorial.php
Next Time...

• Graphs from Exercise 1-4, pgs 15/16 (extra credit
  for computer generated graphs)

• QUIZ (Topics 1 & 13… & 2)

• Topic 2 (Hypotheses and Experiments)

• Lab Report (start on it now)

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Topic 1

  • 1. Principles of Biological Science I Laboratory BSC110L Kristen Covino covinok@gmail.com JST 810
  • 2. Science A Way of Knowing • Systematic knowledge of the world gained through observation and experimentation • Systematic enterprise of gathering knowledge about the world and organizing that knowledge into testable laws and theories • Any systematic knowledge that is capable of resulting in a correct prediction or reliable outcome • Exercise 1-1
  • 3. Biology Living Study of Study of Life
  • 4. Epistemological Cycle Study of Understanding Scientific Method hypothesis prediction question conclusion (accept or reject) experiment observation (gathering data) results
  • 5. Scientific Method 1. Observation Male Red-winged Blackbirds have red wing epaulet, expose epaulet when defending territory Question: Is the red epaulet exposed as a signal or threat to other males? Red-winged Blackbird (Agelaius phoeniceus ) Male Female
  • 6. Scientific Method hypothesis Hypothesis – possible explanation for the observed facts; leads to a testable PREDICTION question observation Red Epaulet as a Threat Signal Hypothesis: Males have red wing epaulet as a signal of threat to other males Prediction: Males without a red epaulet will not be considered a threat to other males
  • 7. Hypothesis: Males have red wing epaulet as a signal of threat to other males Prediction: Males without a red epaulet will not be considered a threat to other males Experiment Hansen and Rohwer (1986) • Manipulated the size of epaulet (double, half, black, normal) • Placed dummy bird in another bird’s territory • Counted the number of times the territorial male hit the dummy bird (hitting dummy bird, signaled the dummy bird was a threat) Double Half Normal Black
  • 8. hypothesis Scientific Method prediction question experiment observation (gathering data) Experiment – designed to test the prediction 1. Define Variables independent variable - variable that is manipulated/changed in the experiment dependent variable – variable being measured, counted, or observed; changes according to the level of the other variable control variables – variables kept constant during the experiment 2. Outline a Procedure Sequence of steps for the experiment (can be designed from published work, collaborations, novel ideas) Replication of the procedure Include a Control – independent variable is held at an established level or omitted
  • 9. Hypothesis: Males have red wing epaulet as a signal of threat to other males Prediction: Males without a red epaulet will not be considered a threat to other males Experiment Hansen and Rohwer (1986) • Manipulated the size of epaulet (double, half, black, normal) • Placed dummy bird in another bird’s territory • Counted the number of times the territorial male hit the dummy bird (hitting dummy bird, signaled the dummy bird was a threat) Double Half Normal Black
  • 10. Types of Observation /Description • Good science depends very heavily on careful observations and accurate descriptions. • Qualitative vs. quantitative descriptions – Qualitative – can be biased, subjective, influenced by opinion or prior experience (examples – color, smell, general size description such as “big”) – Quantitative – can be verified by anyone, objective, nothing left to judgment (example – exact size measurements, weight, facts about distinguishing marks) • Exercise 1-2
  • 11. hypothesis prediction Scientific Method question experiment observation (gathering data) Results – organization and summary of data collected results - Present data collected in tables and figures - help in analyzing and interpreting your results - help a reader or viewer understand your results - no overlap of information among table and figures - Summary of data should be written precisely and accurately, NO fluff Tables and Figures Data Points Title Figure or Table # (with a caption describing content) Y-axis & X-axis labeled (include units if appropriate) Legend (if appropriate)
  • 12. Results – Red-winged Blackbird Experiment Response to Wing Epaulet Size 8 6 Number of Hits (per ten minutes) Table 1.1 Number of hits on mounted red-winged blackbird by territory owner in relation to the size of the red epaulet 4 of the mount Size of Epaulet Number of Hits 2 Double 7 Normal 4 Half 2.2 0 Double Normal Half Black Black 1.8 Wing Epaulet Size Figure 1. Number of hits on mounted red-winged blackbird by territory owners in relation to the size of the red epaulet of the mount.
  • 13. hypothesis prediction Scientific Method question conclusion experiment (accept of reject) observation (gathering data) Conclusion – explanation of results, accept or reject hypothesis, discuss future research results We do NOT prove a hypothesis, we state that the results support our hypothesis Rejecting the hypothesis is just as important as finding support for a hypothesis
  • 14. Conclusion Red-winged Blackbird Conclusion Males without red epaulet are considered less of a threat to territorial males than males with a red epaulet Suggests that the red epaulet is used by males as a signal of threat Red-winged Blackbird (Agelaius phoeniceus )
  • 15. Topic 13 (pages 127-129) How to Write a Scientific Report
  • 16. What is the point of a Parts of a scientific scientific paper? paper: • Communicate information on your experiment in a Abstract clear and concise way Introduction Materials and Methods • Present results to Results scientific community Discussion and/or peers Literature Cited
  • 17. Abstract • A summary of the experiment and its results – 5-7 sentences (2-3 background, 1-2 methods, 2-3 results, 1-2 conclusions) Introduction (2-4 WELL WRITTEN paragraphs) • Background information • Goal of your experiment • State Hypothesis • *Cite literature*
  • 18. Materials and Methods • Describe what you did and the materials that you used so someone else could replicate the experiment • Paragraph form!! No lists!! • Omit unnecessary information – i.e. “We labeled the tubes, “tube 1”, “tube 2”…” “We made graphs using Excel…” Results • General trends in data, NO RAW DATA! • Don’t explain data, just present in paragraph form • Represent data in tables and graphs
  • 19. Graphs • Labels: Title, x-axis, y-axis • Key (optional and depends on the type of data) • Legend (always, Always, ALWAYS!!) One variable graph Figure 1: The number of cars parked in the USM 4 th street Exercise 1-4 parking lot on 24 August, 2011 separated by color.
  • 20. Graphs • Labels: Title, x-axis, y-axis • Key (optional and depends on the type of data) • Legend (always, Always, ALWAYS!!) One variable graph Figure 2: The number of cars parked in the USM 4 th street parking Exercise 1-4 lot on 24 August, and 25 August 2011 separated by color.
  • 21. Graphs • Labels: Title, x-axis, y-axis • Key (optional and depends on the type of graph) • Legend (always, Always, ALWAYS!!) Two variable graph Exercise 1-4 Figure 3: Mass of 10 books on my bookshelf compared to the number of pages in each book.
  • 22. Discussion • 2-4 well written paragraphs • What do you think your results mean? • Did you support or reject your hypothesis? – You cannot prove a hypothesis, if you say this, you will make me angry! • Future questions? • *Cite literature*
  • 23. Literature Cited • In the Literature Cited section: Author, Date. Title. Journal. Volume: Pages. Ex) Smith, A., 1995. Hogsucker diet survey. Fish Food. 18: 25-30. • In the text (Introduction/Discussion): The diet of Hypentilium etowanum, the Alabama hogsucker, consists mainly of insect larvae and small crustaceans (Smith, 1995). Smith (1995) found that the diet of the Alabama hogsucker consists mainly of insect larvae and small crustaceans.
  • 24. Literature Cited • You must cite at least 3 references from scientific journals in your lab report http://www.lib.usm.edu/find_items/article_database s.html Other hints: • Use the rubric to guide you • Length is not as important as QUALITY • Grammar and spell-check!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! • Have someone else read it, or read it aloud • Make it look professional: 10/12pt, normal font choice • PUT YOUR NAME ON IT!!!
  • 25. Today/this week... • Sign Safety Awareness Agreement • Sign e-mail list/”seating” chart • Handouts: Topic 1 supplement & guide to writing scientific papers • Complete Exercises 1-1 , 1-2, & 1-3 • TURN IN lab manual pages 11 – 14 • Online plagiarism tutorial (due by Friday 5pm) http://www.lib.usm.edu/legacy/plag/plagiarismtutorial.php
  • 26. Next Time... • Graphs from Exercise 1-4, pgs 15/16 (extra credit for computer generated graphs) • QUIZ (Topics 1 & 13… & 2) • Topic 2 (Hypotheses and Experiments) • Lab Report (start on it now)

Editor's Notes

  1. Define science. As many or as few words as you want! For our purposes, the short and sweet definition “a way of knowing” encompasses all of that. - “Way” and “Systematic” – insinuate process (scientific method)
  2. How we obtain knowledge
  3. Hypothesis explains the phenomenon you are interested in investigating
  4. What is the dependent, independent, control variables, control, how replicate
  5. What is the dependent, independent, control variables, control, how replicate
  6. More information to help with two of the exercises later in class Way of gathering data that can be most clearly communicated, effective in getting point across, and repeatable - quantitative As scientists, we want to AVOID qualitative
  7. Number consecutively as they appear in text, independent: x-axis, dependent: y-axis Title: Table – above / Figure - below Line graphs vs. Bar graphs
  8. Pages 15 & 16 may be taken home and turned in next week!
  9. Quiz – be on time! Worth 20 points! Detailed questions from this week – exercises and lecture, Vague questions from Topic 2 (pages 17 – 22) Lab report – will give handout of guidelines and suggestions. Follow it and checklists within lab manual!