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Genotyping of 27 Human Papillomavirus Types by Using L1 Consensus PCR Product...
Amplification of human papillomavirus (HPV) DNA by L1 consensus primer systems (e.g., MY09/11 or
GP51/61) can detect as few as 10 to 100 molecules of HPV targets from a genital sample. However, genotype
determination by dot blot hybridization is laborious and requires at least 27 separate hybridizations for
substantive HPV-type discrimination. A reverse blot method was developed which employs a biotin-labeled
PCR product hybridized to an array of immobilized oligonucleotide probes. By the reverse blot strip analysis,
genotype discrimination of multiple HPV types can be accomplished in a single hybridization and wash cycle.
Twenty-seven HPV probe mixes, two control probe concentrations, and a single reference line were immobilized
to 75- by 6-mm nylon strips. Each individual probe line contained a mixture of two bovine serum albuminconjugated
oligonucleotide probes specific to a unique HPV genotype. The genotype spectrum discriminated on
this strip includes the high-risk, or cancer-associated, HPV genotypes 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 55,
56, 58, 59, 68 (ME180), MM4 (W13B), MM7 (P291), and MM9 (P238A) and the low-risk, or non-cancerassociated,
genotypes 6, 11, 40, 42, 53, 54, 57, 66, and MM8 (P155). In addition, two concentrations of b-globin
probes allowed for assessment of individual specimen adequacy following amplification. We have evaluated the
performance of the strip method relative to that of a previously reported dot blot format (H. M. Bauer et al.,
p. 132–152, in C. S. Herrington and J. O. D. McGee (ed.), Diagnostic Molecular Pathology: a Practical Approach,
(1992), by testing 328 cervical swab samples collected in Digene specimen transport medium (Digene Diagnostics,
Silver Spring, Md.). We show excellent agreement between the two detection formats, with 92%
concordance for HPV positivity (kappa 5 0.78, P < 0.001). Nearly all of the discrepant HPV-positive samples
resulted from weak signals and can be attributed to sampling error from specimens with low concentrations
(<1 copy/ml) of HPV DNA. The primary advantage of the strip-based detection system is the ability to rapidly
genotype HPVs present in genital samples with high sensitivity and specificity, minimizing the likelihood of
misclassification.
Type specific persistence of high risk human papillomavirus (HPV) as indicato...
Objectives To investigate the role of human
papillomavirus (HPV) in the development of cervical
neoplasia in women with no previous cervical
cytological abnormalities; whether the presence of
virus DNA predicts development of squamous
intraepithelial lesion; and whether the risk of incident
squamous intraepithelial lesions differs with repeated
detection of the same HPV type versus repeated
detection of different types.
Design Population based prospective cohort study.
Setting General population in Copenhagen,
Denmark.
Participants 10 758 women aged 2029 years
followed up for development of cervical cytological
abnormalities; 370 incident cases were detected (40
with atypical squamous cells of undetermined
significance, 165 with low grade squamous
intraepithelial lesions, 165 with high grade squamous
intraepithelial lesions).
Main outcome measures Results of cervical smear
tests and cervical swabs at enrolment and at the
second examination about two years later.
Results Compared with women who were negative
for human papillomavirus at enrolment, those with
positive results had a significantly increased risk at
follow up of having atypical cells (odds ratio 3.2, 95%
confidence interval 1.3 to 7.9), low grade lesions (7.5,
4.8 to 11.7), or high grade lesions (25.8, 15.3 to 43.6).
Similarly, women who were positive for HPV at the
second examination had a strongly increased risk of
low (34.3, 17.6 to 67.0) and high grade lesions (60.7,
25.5 to 144.0). For high grade lesions the risk was
strongly increased if the same virus type was present
at both examinations (813.0, 168.2 to 3229.2).
Conclusions Infection with human papillomavirus
precedes the development of low and high grade
squamous intraepithelial lesions. For high grade
lesions the risk is greatest in women positive for the
same type of HPV on repeated testing.
The Natural History of Type-specific Human Papillomavirus Infections in Femal...
This study examined the natural history of type-specific HPV infections in female university students over 24 months. Key findings include:
- The incidence rates of acquiring high-risk or low-risk HPV infections were similar at around 14 and 12 cases per 1000 women-months, respectively.
- HPV-16, -51, and -84 had the highest rates of new acquisition at around 5, 3, and 4 cases per 1000 women-months, respectively.
- HPV-16 infections persisted the longest on average at 18 months, followed by HPV-31 and HPV-53 at around 14-15 months. HPV-6 and HPV-84 cleared fastest on average in under 10 months.
- After
Papillomavirus research update: highlights of the Barcelona HPV 2000 internat...
This document summarizes highlights from the 18th International Papillomavirus Conference held in Barcelona, Spain in July 2000. It discusses several key topics:
1) Epidemiological studies presented data on HPV prevalence by age and risk factors like oral contraceptive use.
2) Studies of HPV serology found correlations between antibody levels and sexual behavior or HPV infection status.
3) Research on potential cofactors in cervical cancer like chlamydia infection presented both supporting and non-supporting evidence for their role in carcinogenesis.
Search Engine Optimization (SEO) Training Presentation
David J. Neff, of Ridgewood : Ingenious Communication Strategies, delivered this search engin optimization workshop for the Communications and Web Teams at Best Friends Animal Society.
The astute group quickly grasped how link building, site structure, alt tags, social media, and much more can improve your Google ranking.
Prospects for primary prevention of cervical cancer in developing countries
This document discusses prospects for primary prevention of cervical cancer in developing countries through HPV vaccination. It notes that HPV types 16 and 18 cause the majority of cervical cancers worldwide. Vaccines against HPV, particularly virus-like particle vaccines, show promise in preventing HPV infection and progression to cervical cancer. While efficacy trials of HPV vaccines are underway, effectiveness trials under field conditions in developing countries are also needed to demonstrate the real-world impact of HPV vaccination programs in reducing cervical cancer incidence and mortality over prolonged follow-up periods of 20 years or more. Such effectiveness trials could help accelerate adoption of HPV vaccination in populations that bear the greatest cervical cancer burden.
Recommended
Genotyping of 27 Human Papillomavirus Types by Using L1 Consensus PCR Product...
Amplification of human papillomavirus (HPV) DNA by L1 consensus primer systems (e.g., MY09/11 or
GP51/61) can detect as few as 10 to 100 molecules of HPV targets from a genital sample. However, genotype
determination by dot blot hybridization is laborious and requires at least 27 separate hybridizations for
substantive HPV-type discrimination. A reverse blot method was developed which employs a biotin-labeled
PCR product hybridized to an array of immobilized oligonucleotide probes. By the reverse blot strip analysis,
genotype discrimination of multiple HPV types can be accomplished in a single hybridization and wash cycle.
Twenty-seven HPV probe mixes, two control probe concentrations, and a single reference line were immobilized
to 75- by 6-mm nylon strips. Each individual probe line contained a mixture of two bovine serum albuminconjugated
oligonucleotide probes specific to a unique HPV genotype. The genotype spectrum discriminated on
this strip includes the high-risk, or cancer-associated, HPV genotypes 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 55,
56, 58, 59, 68 (ME180), MM4 (W13B), MM7 (P291), and MM9 (P238A) and the low-risk, or non-cancerassociated,
genotypes 6, 11, 40, 42, 53, 54, 57, 66, and MM8 (P155). In addition, two concentrations of b-globin
probes allowed for assessment of individual specimen adequacy following amplification. We have evaluated the
performance of the strip method relative to that of a previously reported dot blot format (H. M. Bauer et al.,
p. 132–152, in C. S. Herrington and J. O. D. McGee (ed.), Diagnostic Molecular Pathology: a Practical Approach,
(1992), by testing 328 cervical swab samples collected in Digene specimen transport medium (Digene Diagnostics,
Silver Spring, Md.). We show excellent agreement between the two detection formats, with 92%
concordance for HPV positivity (kappa 5 0.78, P < 0.001). Nearly all of the discrepant HPV-positive samples
resulted from weak signals and can be attributed to sampling error from specimens with low concentrations
(<1 copy/ml) of HPV DNA. The primary advantage of the strip-based detection system is the ability to rapidly
genotype HPVs present in genital samples with high sensitivity and specificity, minimizing the likelihood of
misclassification.
Type specific persistence of high risk human papillomavirus (HPV) as indicato...
Objectives To investigate the role of human
papillomavirus (HPV) in the development of cervical
neoplasia in women with no previous cervical
cytological abnormalities; whether the presence of
virus DNA predicts development of squamous
intraepithelial lesion; and whether the risk of incident
squamous intraepithelial lesions differs with repeated
detection of the same HPV type versus repeated
detection of different types.
Design Population based prospective cohort study.
Setting General population in Copenhagen,
Denmark.
Participants 10 758 women aged 2029 years
followed up for development of cervical cytological
abnormalities; 370 incident cases were detected (40
with atypical squamous cells of undetermined
significance, 165 with low grade squamous
intraepithelial lesions, 165 with high grade squamous
intraepithelial lesions).
Main outcome measures Results of cervical smear
tests and cervical swabs at enrolment and at the
second examination about two years later.
Results Compared with women who were negative
for human papillomavirus at enrolment, those with
positive results had a significantly increased risk at
follow up of having atypical cells (odds ratio 3.2, 95%
confidence interval 1.3 to 7.9), low grade lesions (7.5,
4.8 to 11.7), or high grade lesions (25.8, 15.3 to 43.6).
Similarly, women who were positive for HPV at the
second examination had a strongly increased risk of
low (34.3, 17.6 to 67.0) and high grade lesions (60.7,
25.5 to 144.0). For high grade lesions the risk was
strongly increased if the same virus type was present
at both examinations (813.0, 168.2 to 3229.2).
Conclusions Infection with human papillomavirus
precedes the development of low and high grade
squamous intraepithelial lesions. For high grade
lesions the risk is greatest in women positive for the
same type of HPV on repeated testing.
The Natural History of Type-specific Human Papillomavirus Infections in Femal...
This study examined the natural history of type-specific HPV infections in female university students over 24 months. Key findings include:
- The incidence rates of acquiring high-risk or low-risk HPV infections were similar at around 14 and 12 cases per 1000 women-months, respectively.
- HPV-16, -51, and -84 had the highest rates of new acquisition at around 5, 3, and 4 cases per 1000 women-months, respectively.
- HPV-16 infections persisted the longest on average at 18 months, followed by HPV-31 and HPV-53 at around 14-15 months. HPV-6 and HPV-84 cleared fastest on average in under 10 months.
- After
Papillomavirus research update: highlights of the Barcelona HPV 2000 internat...
This document summarizes highlights from the 18th International Papillomavirus Conference held in Barcelona, Spain in July 2000. It discusses several key topics:
1) Epidemiological studies presented data on HPV prevalence by age and risk factors like oral contraceptive use.
2) Studies of HPV serology found correlations between antibody levels and sexual behavior or HPV infection status.
3) Research on potential cofactors in cervical cancer like chlamydia infection presented both supporting and non-supporting evidence for their role in carcinogenesis.
Search Engine Optimization (SEO) Training Presentation
David J. Neff, of Ridgewood : Ingenious Communication Strategies, delivered this search engin optimization workshop for the Communications and Web Teams at Best Friends Animal Society.
The astute group quickly grasped how link building, site structure, alt tags, social media, and much more can improve your Google ranking.
Prospects for primary prevention of cervical cancer in developing countries
This document discusses prospects for primary prevention of cervical cancer in developing countries through HPV vaccination. It notes that HPV types 16 and 18 cause the majority of cervical cancers worldwide. Vaccines against HPV, particularly virus-like particle vaccines, show promise in preventing HPV infection and progression to cervical cancer. While efficacy trials of HPV vaccines are underway, effectiveness trials under field conditions in developing countries are also needed to demonstrate the real-world impact of HPV vaccination programs in reducing cervical cancer incidence and mortality over prolonged follow-up periods of 20 years or more. Such effectiveness trials could help accelerate adoption of HPV vaccination in populations that bear the greatest cervical cancer burden.
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The Hubble Space Telescope is a large space-based observatory named after astronomer Edwin Hubble that has provided unprecedented views of the universe. Hubble orbits Earth every 96 minutes and has taken many famous images including pillars of creation in the Eagle Nebula and the Crab Nebula. Some of Hubble's images show planetary nebulae, star clusters, galaxies and more distant objects in the universe.
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This document provides tips on the health benefits of various fruits and vegetables. It lists benefits such as protecting the heart, preventing constipation, combating cancer, controlling blood pressure, improving skin health, strengthening bones, and more. It also provides recipes for healthy fruit and vegetable juices and their associated benefits such as improving skin complexion, reducing body heat, and strengthening immunity. The overall message is to take care of your health by eating fruits and vegetables for their many nutritional benefits.
Top 10 Transparent Animals
There are many transparent animals that live in oceans around the world, including glass frogs, barreleye fish, glasswing butterflies, glass squid, transparent zebrafish, icefish, amphipods, larval shrimp, salps, and jellyfish. These animals' transparent or translucent bodies allow scientists to study their internal organs and biological processes in vivo, and serve as camouflage for predators and prey in deep ocean environments with little light.
Iman Maleki Pinturas
El pintor iraní Imán Maleki ha recibido varios premios importantes de arte por su estilo realista, incluyendo el premio William Bouguereau y el premio "Chairman's Choice". Nació en 1976 en Teherán e Irán y estudió con el mejor pintor hiperrealista de Irán. Sus pinturas muestran aspectos de la vida cotidiana del pueblo iraní con un alto nivel de detalle y realismo.
A Friend's Greetings
The poem expresses the speaker's wish to be as good a friend to the recipient as they have been to the speaker. The speaker wants to help and support the recipient as much as the recipient has helped them, and to make the recipient feel as happy and uplifted as the recipient has made the speaker feel. The document also lists the symbolic meanings of different colored roses and what each color represents - including friendship, love, wealth, knowledge, success, happiness, beauty, honesty, family and health.
What Is Your Name In Over 330 Languages
This document contains phrases in many different languages that ask "What is your name?". It lists the language, country or region, and provides the translation of that phrase in each respective language. There are over 200 entries in total that cover languages from around the world.
Hello In Many Languages
This document contains greetings in many different languages from around the world. It lists over 200 greetings phrases translated into English, along with the original language. The greetings cover languages from regions including Africa, Asia, Europe, North and South America, and the Pacific Islands.
Quotes
The document contains a collection of quotes about life, dreams, happiness, success, and human relationships. Some of the quotes encourage living in the present and making the most of each day, as well as believing in oneself and one's dreams. Other quotes discuss the importance of listening to opportunities, building bridges with others rather than walls, and finding happiness in appreciating what one already has.
Meet Croatia through pictures
Croatia is a beautiful country located on the Adriatic Sea with over 1,000 islands and coastlines. It has a rich history and culture dating back to ancient times. The country offers stunning natural scenery ranging from mountains and forests to beaches and seaside towns, along with medieval architecture and historic sites that make it a popular tourist destination.
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Cosmology And Astronomy
The Hubble Space Telescope is a large space-based observatory named after astronomer Edwin Hubble that has provided unprecedented views of the universe. Hubble orbits Earth every 96 minutes and has taken many famous images including pillars of creation in the Eagle Nebula and the Crab Nebula. Some of Hubble's images show planetary nebulae, star clusters, galaxies and more distant objects in the universe.
Amazing Foodscape Scenery
The British photographer Carl Warner created a series of landscape images utilizing basic food ingredients found in a typical Kitchen.
Health - very important tips
This document provides tips on the health benefits of various fruits and vegetables. It lists benefits such as protecting the heart, preventing constipation, combating cancer, controlling blood pressure, improving skin health, strengthening bones, and more. It also provides recipes for healthy fruit and vegetable juices and their associated benefits such as improving skin complexion, reducing body heat, and strengthening immunity. The overall message is to take care of your health by eating fruits and vegetables for their many nutritional benefits.
Top 10 Transparent Animals
There are many transparent animals that live in oceans around the world, including glass frogs, barreleye fish, glasswing butterflies, glass squid, transparent zebrafish, icefish, amphipods, larval shrimp, salps, and jellyfish. These animals' transparent or translucent bodies allow scientists to study their internal organs and biological processes in vivo, and serve as camouflage for predators and prey in deep ocean environments with little light.
Iman Maleki Pinturas
El pintor iraní Imán Maleki ha recibido varios premios importantes de arte por su estilo realista, incluyendo el premio William Bouguereau y el premio "Chairman's Choice". Nació en 1976 en Teherán e Irán y estudió con el mejor pintor hiperrealista de Irán. Sus pinturas muestran aspectos de la vida cotidiana del pueblo iraní con un alto nivel de detalle y realismo.
A Friend's Greetings
The poem expresses the speaker's wish to be as good a friend to the recipient as they have been to the speaker. The speaker wants to help and support the recipient as much as the recipient has helped them, and to make the recipient feel as happy and uplifted as the recipient has made the speaker feel. The document also lists the symbolic meanings of different colored roses and what each color represents - including friendship, love, wealth, knowledge, success, happiness, beauty, honesty, family and health.
What Is Your Name In Over 330 Languages
This document contains phrases in many different languages that ask "What is your name?". It lists the language, country or region, and provides the translation of that phrase in each respective language. There are over 200 entries in total that cover languages from around the world.
Hello In Many Languages
This document contains greetings in many different languages from around the world. It lists over 200 greetings phrases translated into English, along with the original language. The greetings cover languages from regions including Africa, Asia, Europe, North and South America, and the Pacific Islands.
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Croatia is a beautiful country located on the Adriatic Sea with over 1,000 islands and coastlines. It has a rich history and culture dating back to ancient times. The country offers stunning natural scenery ranging from mountains and forests to beaches and seaside towns, along with medieval architecture and historic sites that make it a popular tourist destination.
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Tibet
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