The TCA cycle is linked to- i- The urea cycle by the molecule Fumarate.pdf
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The TCA cycle is linked to the urea cycle through the molecule fumarate. It is also linked to beta-oxidation of fatty acids through an unnamed molecule. The document lists two ways the TCA cycle is linked to other metabolic pathways but does not provide further details.
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environment. E. coli is not a naturally competent species, but it can be induced to "pick up".
DNA to a limited extent after treatment with calcium ( Ca ++ ) ions and by "heat-shock" or short
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pBSKS. It is an artificial plasmid generated by using recombinant DNA techniques. It has a gene
for resistance to the antibiotic ampicillin. Normally, this antibiotic does not allow E . coli
bacteria to grow. However, transformed bacteria containing the plasmid will be resistant to the
antibiotic and will grow in selective medium containing ampicillin. The strain of E. coli you will
use is called JM109. This particular strain is L a c - because it carries a mutant allele of the lacZ
gene encoding galactosidase. The bacteria produces a defective form of the enzyme and would
not survive in a medium in which lactose were used as the only carbon source. The pBSKS
plasmid contains a segment of the lacZ gene. This fragment is capable of complementing the
lacZ mutant allele in the chromosomal DNA. This is an example of intragenic complementation.
JM109 cells transformed with pBSKS produce a functional form of -galactosidase and would
survive on medium containing lactose as the sole carbon source. You could test if your c.
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