The travel time for a college student traveling between her home and h.pdf
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The travel time for a college student traveling between her home and her college is uniformly distributed between 20 and 50 minutes. What is the probability thal her trip will take exactly 30 minutes? 0 0.0333 0.3333 0.9667.
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medium by cells. Fred Griffith demonstrated transformation in 1928 when he exposed mice to
two different strains of the bacterium Streptococcus pneumoniae. When he injected mice with a
mix of killed, virulent S-strain bacteria and live, nonvirulent R-strain bacteria, the mice died of
pheumonia, and live S-strain bacteria were found in their bodies. Clearly, the R-strain bacteria
had picked up DNA from the dead Sstrain, and in so doing they were transformed into a virulent
strain. The tranformation was possible because Streptococcus bacteria are competent. Competent
bacterial cells are those that have the ability to pick up DNA from the environment. Many genera
of bacteria are naturally competent. But competency may be influenced by various factors,
including temperature changes and the concentration of different ions present in the
environment. E. coli is not a naturally competent species, but it can be induced to "pick up".
DNA to a limited extent after treatment with calcium ( Ca ++ ) ions and by "heat-shock" or short
exposure to higher temperatures ( 4 2 C ) . You will use plasmid DNA to transform E . coli.
Plasmids are selfreplicating, double-stranded, circular DNA molecules that are maintained in
bacteria as independent extra-chromosomal entities. Virtually all bacterial genera have plasmids.
Some plasmids carry information for their own transfer from one cell to another (F plasmids),
others may encode resistance to antibiotics. Plasmids can range in size from less than 1 to more
than 500 kb . Some plasmids (low-copy plasmids) are present in low numbers, 1 to 4 copies per
cell, while others are present in 10 to 100 copies per cell, and are called high-copy-number
plasmids. Plasmids are good vectors to introduce DNA into bacterial cells, for cloning or
amplification purposes. The plasmid you will be using for E . coli transformation is called
pBSKS. It is an artificial plasmid generated by using recombinant DNA techniques. It has a gene
for resistance to the antibiotic ampicillin. Normally, this antibiotic does not allow E . coli
bacteria to grow. However, transformed bacteria containing the plasmid will be resistant to the
antibiotic and will grow in selective medium containing ampicillin. The strain of E. coli you will
use is called JM109. This particular strain is L a c - because it carries a mutant allele of the lacZ
gene encoding galactosidase. The bacteria produces a defective form of the enzyme and would
not survive in a medium in which lactose were used as the only carbon source. The pBSKS
plasmid contains a segment of the lacZ gene. This fragment is capable of complementing the
lacZ mutant allele in the chromosomal DNA. This is an example of intragenic complementation.
JM109 cells transformed with pBSKS produce a functional form of -galactosidase and would
survive on medium containing lactose as the sole carbon source. You could test if your c.
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