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Similar to The pancreas is both an endocrine and exocrine gland. It is located in the curve of the duodenum. Pancreas. Copyright © 2014 John Wiley & Sons, Inc. All rights
Similar to The pancreas is both an endocrine and exocrine gland. It is located in the curve of the duodenum. Pancreas. Copyright © 2014 John Wiley & Sons, Inc. All rights (20)
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The pancreas is both an endocrine and exocrine gland. It is located in the curve of the duodenum. Pancreas. Copyright © 2014 John Wiley & Sons, Inc. All rights
- 1. PANCREAS ANATOMY stomach esophagus ductus choledocus duodenum duct of Santorini duct of Wirsung pancreas
- 3. PANCREATIC SECRETIONS 1. PROTEASES (70%) Endopeptidases (trypsin, chymotrypsin, elastases) Exopeptidases (carboxypeptidases) trypsinogen trypsin activates all other precursors enterokinase (duct walls) 2. NUCLEASES (DNAase, RNAase) 3. PANCREATIC AMYLASE (hydrolyse starch and gl;ycogen) 4. PANCREATIC LIPASE (triglycerides fatty acids and glycerol)
- 4. REGULATION OF SECRETION 1. SECRETIN food in stomach release of stomach acid into duodenum release of secretin into blood by duodenal cells secretion of bicarbonate by duct cells alkaline pH (ideal for pancreatic enzymes) 2. PANCREOZYMIN (cholecystokinen) amino acids and fats in intestine release of pancreozymin by intestinal mucosa into blood relase of pancreatic enzymes into the intestine 3. GASTRIN food in stomach gastrin secretion by stomach mucosa release of pancreatic enzymes into the intestine 4. AUTONOMIC INNERVATION (vagus nerve)
- 5. PANCREAS (PANORAMIC) pancreatic acini islet of Langerhans islet of Langerhans BV pancreatic lobe
- 6. PANCREATIC SECRETORY DUCTS LARGE SECRETORY DUCT INTERLOBULAR DUCT acinus simple cuboidal epithelium simple columnar epithelium
- 7. EXOCRINE PANCREAS interlobular duct intercalated duct nerve acinus pyramidal secretory cell basal basophilia (rough ER) zymogen granules centro- acinar cells blood vessel rough endoplasmic reticulum golgi apparatus golgi vesicles condensing vacuoles zymogen granules merocrine secretion (exocytosis)
- 8. INTERCALARY DUCTS AND CENTROACINAR CELLS intercalary duct intercalary duct centroacinar cell
- 9. PANCREATIC EXOCRINE SECRETORY CELL zymogen granules centroacinar cell
- 10. CELL BIOLOGY OF PANCREATIC SECRETION 1. SYNTHESIS 14 C -leucine + tRNA 14 C-amino-acyl tRNA mRNA for chymotrysinogen ribosomes chymotrysinogen 2. SEGREGATION mRNA ribosomes polypeptide RER cisterna 3. INTRACELLULAR TRANSPORT RER transitional elements golgi apparatus golgi vesicles 4. CONCENTRATION H2O H2O condensing vacuoles 5. INTRACELLULAR STORAGE zymogen granules 6. DISCHARGE exocytosis
- 11. EXPERIMENTAL METHODS IN CELL SECRETION 1. SUBCELLULAR FRACTIONATION (grind and find) density gradient centrifugation zymogen granules condensing vacuoles golgi microsomes (RER) vesicles cpm 14 C leucine cpm 14 C leucine cpm 14 C leucine RER golgi cond zymogen RER golgi cond zymogen RER golgi cond zymogen (3 mins) (7 mins) (80 mins) pulse chase 2. RADIOAUTOGRAPHY (cook and look) developed silver grains photographic emulsion ultrathin section pancreatic acinar cell
- 12. ISLET OF LANGERHANS alpha cell beta cell blood capillary acinus beta cell alpha cell fenestrated capillary (insulin) (glucagon) vascular pole (secretion by exocytosis) INSULIN- increases membrane permeability to glucose promotes glycogenesis decrease blood glucose levels GLUCAGON - promotes glycogenolysis increases blood glucose levels “ ANTAGONISTIC HORMONES”
- 14. ISLET OF LANGERHANS - TEM
- 15. STAINED ALPHAAND BETA CELLS ALPHA CELLS BETA CELLS