This document summarizes research on the expression of E-cadherin and N-cadherin in cultures of bovine corneal epithelium and endothelium. It states that cadherins are important cell-cell adhesion proteins that form adherens junction complexes. The development of E-cadherin in corneal epithelial cells and N-cadherin in endothelial cells starts between 2-4 days after seeding the cells in culture, as shown through immunofluorescence and western blot analyses. Transepithelial electrical resistance measurements also increase significantly over the first 4-6 days as cadherins form adherens junctions between the cells.
EXTRACTION AND CLASSIFICATION OF BLEBS IN HUMAN EMBRYONIC STEM CELLdbpublications
A main objective of this paper is
to extract bleb from the human
embryonic stem cells. Blebbing is an
important biological indicator in
determining the health of human
embryonic stem cells (hESC). Especially,
areas of a bleb sequence in a video are
often used to distinguish two cells
blebbing behaviours in HESC; dynamic
and apoptotic blessings. Here analyses
active contour segmentation method for
bleb extraction in hESC videos and
introduces a bio-inspired score function
to improve the performance in bleb
extraction. The full bleb formation
consists of bulb expansion and retraction.
Blebs change their size and image
properties dynamically in both processes
and between frames. Therefore, adaptive
parameters are needed for each
segmentation method. A score function
derived from the change of bleb area and
orientation between consecutive frames with cuckoo optimization is proposed
which provides adaptive parameters for
bleb extraction in videos and classified
using artificial neural networks (ANN).
DNA
INTRODUCTION
CHEMICAL COMPOSITION
NUCLEOSIDES & NUCLEOTIDES
DNA REPAIR
INTRODUCTION
TYPES OF DNA REPAIR
I)DIRECT REPAIR SYSTEM,
II)BASE EXCISION REPAIR,
III)NUCLEOTIDE EXCISION REPAIR,
IV)MISMATCH REPAIR,
V)RECOMBINATION REPAIR,
DEFECTS IN DNA REPAIR UNDERLIE HUMAN DISEASE
DNA RECOMBINATION
INTRODUCTION
MECHANISM OF DNA RECOMBINATION
TYPES OF RECOMBINATION
I) HOMOLOGOUS RECOMBINATION
MODELS FOR HOMOLOGOUS RECOMBINATION:-
I)HOLLIDAY MODEL,
II)MESSELSON AND RADDING MODEL,
III)DOUBLE STRAND BREAK MODEL,
GENE CONVERSION
II) NON-HOMOLOGOUS RECOMBINATION,
i) SITE SPECIFIC RECOMBINATION,
ii)TRANSPOSITIONAL RECOMBINATION.,
3DSIG 2014 Presentation: Systematic detection of internal symmetry in proteinsSpencer Bliven
These slides are from 3DSIG 2014, presented on July 11.
I describe our investigation of internal symmetry in protein structures. This is quite common (24% of domains), and has many implications for function, folding, and evolution.
I introduce the CE-Symm method, described in
Myers-Turnbull, D., Bliven, S. E., Rose, P. W., Aziz, Z. K., Youkharibache, P., Bourne, P. E., & Prlić, A. (2014). Systematic Detection of Internal Symmetry in Proteins Using CE-Symm. Journal of Molecular Biology, 426(11), 2255–2268. doi:10.1016/j.jmb.2014.03.010
I discuss the results from running CE-Symm across the PDB, as well as some particularly compelling examples.
See also my poster by the same title for more details.
Delineating Recombination Frequency Between Methicillin Resistant and Suscept...JR Matthews
Resistance to antibiotics can occur either by mutation or by acquisition of resistance conferring genes via horizontal gene transfer (HGT), of which the latter is considered to be the most important factor in the current pandemic of antimicrobial resistance genes1. Pathogenic Staphylococcus aureus is an adept bacteria that becomes more dangerous with a strain’s procurement of the SCCmec complex. This provides multiple antibiotic resistance features rendering methicillin and most other beta-lactams useless in the fight against this pathogen. Utilizing computational methods, this study investigates methicillin resistant and methicillin susceptible bacteremia to elucidate the relationship between frequencies of recombination events and horizontally acquired antibiotic resistant genes. We hypothesized that methicillin resistant (R) strains will experience homologous recombination more frequent than methicillin susceptible (S) strains and therefore have a positive correlation with the number of antibiotic resistant genes present in the genome. Using a collection of patient blood samples, diagnosed with Staphylococcus aureus bacteremia, and computational biology to infer parameters of recombination. We examined the genomes for antibiotic resistance genes known to be gained through recombination. In clusters that were analyzed, R strains showed that sample diversity of genomes are greater than S strains and that a greater percentage of the genome is from recombination, respectively. Phylogenetic sequence cluster’s (SC) R genomes had more median recombination divergence than the SC S genomes, (SC5R = 0.15 nt, SC5S = 0.025 nt) and (SC8R = 0.048 nt SC8S = 0.044 nt) per locus. These same methicillin resistant SC genomes contained two and a half times and two times as many antibiotic resistant genes than its methicillin susceptible SC genomes, on average, (SC5R = 12, SC5S =5) and (SC8R =10, SC8S = 6).
văn bản hợp nhất 07/VBHN-BCT ngày 17/1/2017 về phân bón Thư Nguyễn
Ngày 17/1/2017 Bộ Công Thương ban hành văn bản hợp nhất 07/VBHN-BCT Thông tư quy định cụ thể và hướng dẫn thực hiện một số điều về phân bón vô cơ, hướng dẫn việc cấp phép sản xuất phân bón vô cơ đồng thời sản xuất phân bón hữu cơ và phân bón khác tại Nghị định số 202/2013/NĐ-CP ngày 27/11/2013 của Chính phủ về quản lý phân bón
EXTRACTION AND CLASSIFICATION OF BLEBS IN HUMAN EMBRYONIC STEM CELLdbpublications
A main objective of this paper is
to extract bleb from the human
embryonic stem cells. Blebbing is an
important biological indicator in
determining the health of human
embryonic stem cells (hESC). Especially,
areas of a bleb sequence in a video are
often used to distinguish two cells
blebbing behaviours in HESC; dynamic
and apoptotic blessings. Here analyses
active contour segmentation method for
bleb extraction in hESC videos and
introduces a bio-inspired score function
to improve the performance in bleb
extraction. The full bleb formation
consists of bulb expansion and retraction.
Blebs change their size and image
properties dynamically in both processes
and between frames. Therefore, adaptive
parameters are needed for each
segmentation method. A score function
derived from the change of bleb area and
orientation between consecutive frames with cuckoo optimization is proposed
which provides adaptive parameters for
bleb extraction in videos and classified
using artificial neural networks (ANN).
DNA
INTRODUCTION
CHEMICAL COMPOSITION
NUCLEOSIDES & NUCLEOTIDES
DNA REPAIR
INTRODUCTION
TYPES OF DNA REPAIR
I)DIRECT REPAIR SYSTEM,
II)BASE EXCISION REPAIR,
III)NUCLEOTIDE EXCISION REPAIR,
IV)MISMATCH REPAIR,
V)RECOMBINATION REPAIR,
DEFECTS IN DNA REPAIR UNDERLIE HUMAN DISEASE
DNA RECOMBINATION
INTRODUCTION
MECHANISM OF DNA RECOMBINATION
TYPES OF RECOMBINATION
I) HOMOLOGOUS RECOMBINATION
MODELS FOR HOMOLOGOUS RECOMBINATION:-
I)HOLLIDAY MODEL,
II)MESSELSON AND RADDING MODEL,
III)DOUBLE STRAND BREAK MODEL,
GENE CONVERSION
II) NON-HOMOLOGOUS RECOMBINATION,
i) SITE SPECIFIC RECOMBINATION,
ii)TRANSPOSITIONAL RECOMBINATION.,
3DSIG 2014 Presentation: Systematic detection of internal symmetry in proteinsSpencer Bliven
These slides are from 3DSIG 2014, presented on July 11.
I describe our investigation of internal symmetry in protein structures. This is quite common (24% of domains), and has many implications for function, folding, and evolution.
I introduce the CE-Symm method, described in
Myers-Turnbull, D., Bliven, S. E., Rose, P. W., Aziz, Z. K., Youkharibache, P., Bourne, P. E., & Prlić, A. (2014). Systematic Detection of Internal Symmetry in Proteins Using CE-Symm. Journal of Molecular Biology, 426(11), 2255–2268. doi:10.1016/j.jmb.2014.03.010
I discuss the results from running CE-Symm across the PDB, as well as some particularly compelling examples.
See also my poster by the same title for more details.
Delineating Recombination Frequency Between Methicillin Resistant and Suscept...JR Matthews
Resistance to antibiotics can occur either by mutation or by acquisition of resistance conferring genes via horizontal gene transfer (HGT), of which the latter is considered to be the most important factor in the current pandemic of antimicrobial resistance genes1. Pathogenic Staphylococcus aureus is an adept bacteria that becomes more dangerous with a strain’s procurement of the SCCmec complex. This provides multiple antibiotic resistance features rendering methicillin and most other beta-lactams useless in the fight against this pathogen. Utilizing computational methods, this study investigates methicillin resistant and methicillin susceptible bacteremia to elucidate the relationship between frequencies of recombination events and horizontally acquired antibiotic resistant genes. We hypothesized that methicillin resistant (R) strains will experience homologous recombination more frequent than methicillin susceptible (S) strains and therefore have a positive correlation with the number of antibiotic resistant genes present in the genome. Using a collection of patient blood samples, diagnosed with Staphylococcus aureus bacteremia, and computational biology to infer parameters of recombination. We examined the genomes for antibiotic resistance genes known to be gained through recombination. In clusters that were analyzed, R strains showed that sample diversity of genomes are greater than S strains and that a greater percentage of the genome is from recombination, respectively. Phylogenetic sequence cluster’s (SC) R genomes had more median recombination divergence than the SC S genomes, (SC5R = 0.15 nt, SC5S = 0.025 nt) and (SC8R = 0.048 nt SC8S = 0.044 nt) per locus. These same methicillin resistant SC genomes contained two and a half times and two times as many antibiotic resistant genes than its methicillin susceptible SC genomes, on average, (SC5R = 12, SC5S =5) and (SC8R =10, SC8S = 6).
văn bản hợp nhất 07/VBHN-BCT ngày 17/1/2017 về phân bón Thư Nguyễn
Ngày 17/1/2017 Bộ Công Thương ban hành văn bản hợp nhất 07/VBHN-BCT Thông tư quy định cụ thể và hướng dẫn thực hiện một số điều về phân bón vô cơ, hướng dẫn việc cấp phép sản xuất phân bón vô cơ đồng thời sản xuất phân bón hữu cơ và phân bón khác tại Nghị định số 202/2013/NĐ-CP ngày 27/11/2013 của Chính phủ về quản lý phân bón
Quy chuẩn kỹ thuật quốc gia QCVN 105:2016/BTTTT về thiết bị vô tuyến trong ng...Thư Nguyễn
Quy chuẩn kỹ thuật quốc gia QCVN 105:2016/BTTTT về thiết bị vô tuyến trong nghiệp vụ di động hàng không băng tần 117,975-137 MHz dùng trên mặt đất sử dụng điều chế AM
QUY CHUẨN KỸ THUẬT QUỐC GIA QCVN 103:2016/BTTTT VỀ TƯƠNG THÍCH ĐIỆN TỪ ĐỐI VỚ...Thư Nguyễn
QUY CHUẨN KỸ THUẬT QUỐC GIA QCVN 103:2016/BTTTT VỀ TƯƠNG THÍCH ĐIỆN TỪ ĐỐI VỚI THIẾT BỊ TRẠM GỐC, LẶP VÀ PHỤ TRỢ TRONG HỆ THỐNG THÔNG TIN DI ĐỘNG GSM, W-CDMA FDD VÀ LTE
Firme specializate în foraje puturi si foraje orizontale la dispoziția dumneavoastră ! Euforaje.ro prezinta executanti, echipamente, materiale si utilaje la doar un click distanță.
The ECIS is a turnkey system that provides researchers with an advanced, automated, non-invasive means to monitor cell behavior in real-time and without the use of labels.
Cornea is the outermost anterior layer of the eye ball which
has unique optical and physio-mechanical properties to maintain
good vision. A stratified squamous epithelium is continued with the conjunctival epithelium in the limbal area and create a protective stratum on the ocular surface to defend internal structure from environmental threats
DNA and Cell Reprogramming via Epigenetic Information Delivered by Magnetic Fields, Sound Vibrations and Coherent Water.
Don't Miss Dr. Carlo Ventura's presentation at the 2016 Conference for Consciousness & Human Evolution, a series of talks designed to give you tools to develop your consciousness. Click Here to Learn More
1. Expression of E- and N- cadherin in cultures of bovine
corneal epithelium and endothelium
2. A tissue-engineered cornea based on untransformed
corneal cells, should mimic the properties of the
native cornea.
In addition to use as corneal replacement, a corneal
equivalent could be a useful model for studying
wound healing, physiology, toxicology, and
pharmacology.
3. - Cadherins are a vast super family of cell-cell adhesion proteins.
-calcium dependent cell membrane proteins and exist in cadherin-catenin
complex. (adherens junction proteins)
-with three subfamilies.
-roles: cell-cell contact, Tissue morphogenesis, inhibits cancer development
http://www.hindawi.com/journals/vmi/2012/357187/fig1/
4. -Corneal epithelium is a polarized tissue with distinct plasma membrane on its
apical and basolateral domains( dependent on adherens junction complexes).
-N-cadherin is essential for maintaining proper structure of corneal endothelial
AJCs. Its ablation leads to increased endothelial permeability and corneal edema in
mature eyes.
5. According to some experiments (Giasson et.al 2014) corneal equivalent by seeding
fibroblasts into a collagen gel on which epithelial and endothelial cells were added on
each side, cells from the epithelial and endothelial layers expressed adherens junction
proteins, indicating the presence of cell–cell contacts.
Mol Vis. 2014; 20: 386–394.
Published online 2014 Mar 29.
6. The development of, E-cadherin and N-cadherin in
corneal epithelial and endothelial cell culture ,starts in
2-4 days after seeding of the corneal epithelial and
endothelial cells .
7. 1-Separation and isolation of bovine corneal cells
2-subculture and feeding the cells
3- Passage of epithelium and endothelial cells
4-transfer the cells to 6-well
5-they were lifted to the liquid-air interface
6-TER measurement
7-Treatment of membrane and immunofluorescence
8-SDS-PAGE and western blots*
8.
9.
10. Measurement of electrical resistance
Electrical resistance measurements as a measurement of cell-
cell junctions, was used to determine the situation of
adherence junctions of the cells.
Int Poster J Dent Oral Med 3 (2001), No.
2 (15.06.2001)
11. IF and WB and TER will be repeated at 2, 4,6 and days
after seeding
Each experiment will be repeated 3 or 4 times
Observation of IF and WB at these times: cadherins
formed?
TER: Mean Resistance at each time will be plotted.
Differences in mean resistance will be tested for
statistical significance with a repeated measure one
way analysis of variance (time)(ANOVA).
13. Cadherins and a-,b-catenins will be increasingly
expressed with time after seeding of epi and endo cells
by observation of IF and Western blots
N- and E-cadherins will be only expressed in
endothelial and epithelial corneal cells, respectively
Cadherins and catenins are expressed after 3-4 days
after seeding of corneal epithelial and endothelial cells
in monoculture
14. 1. Nelson WJ, Adams CL, Chen YT, Smith SJ. Epithelial cell polarity from the outside looking in. News
Physiol Sci. 2003;18:143–6. [PMC free article] [PubMed]
2-Vassil S. Vassilev, Michiko Mandai, Shigenobu Yonemura, Masatoshi Takeichi .Loss of N-Cadherin from
the Endothelium Causes Stromal Edema and Epithelial Dysgenesis in the Mouse Cornea.Investigative
Ophthalmology & Visual Science October 2012, Vol.53, 7183-7193. doi:10.1167/iovs.12-9949
3-Claud J.Giasson, Alexandre Deschambeault, Patrick Carrier and Luice Germain. Adherens junction
proteins are expressed in collagen corneal equivalents produced in vitro with human cells. Mol Vis. 2014;
20: 386–394.
Published online 2014 Mar 29.
Editor's Notes
The observation of cultured cancer cells in vitro, showed that the first action in cancer cells is loss of
contact inhibition(CADHERINS) and the second step is being invasion and metastasis form.
-E-cadherins expressed in corneal equivalent (IF)
-3 weeks after seeding the cells
* sodium dodecyl sulfate polyacrylamide gel electrophoresis
Pen-strep solution(0.4%) ,
assay BCA(bicinchoninic acid assay ) is a biochemical assay for determining the total concentration of protein in a solution (0.5 μg/mL to 1.5 mg/mL). The total protein concentration is exhibited by a color change of the sample solution from green to purple in proportion to protein concentration, which can then be measured using colorimetric techniques.
Classical cadherins has the appropriate molecular weights of 120–130 kDa.
Blots exposed to ECL reagents for chemiluminescence.
Columns :TER KΩ
Rows : days
Mean and Standard deviation
Cornelal endothelial cell culture, in third passage.