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The effect of xylopia Ethiopica plants on the serum lipid level of a pregnant wistar rat
- 1. A PROJECT TOPIC ONTHE EFFECT OF XYLOPIA AETHIOPICA PLANTS ON THE SERUM LIPIDS LEVEL OF PREGNANT WISTAR RATS BY MORDI BLESSING CHUKWUDUMEBI BMS1802615 DEPARTMENT OF PHYSIOLOGY, SCHOOL OF BASIC MEDICAL SCIENCES, UNIVERSITY OF BENIN, BENIN CITY SUPERVISED BY MRS. G.N. AZEKHUMEN OCTOBER, 2023. 1
- 2. COURSE OUTLINE Introduction Aims Objectives ofthis research study Experimental design Materials & Methodology Results/Discussion Conclusion 2
- 3. INTRODUCTION BACKGROUND OFTHIS STUDY Xylopia Aethiopica(pepper soup spice), commonly known as African pepper or Ethiopian pepper. 3
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- 5. INTRODUCTION CONTD.... Serum lipidsrefers to the liquid components found in the liquid portion of blood known as serum. Examples of serum lipids are cholesterol, triglycerides, lipoproteins, phospholipids (James et al., 2018).
- 6. AIMS AND OBJECTIVES AIMOF THIS STUDY The aim of this research is to determine the effect of xylopia Aethiopica supplementation on serum lipids levels in pregnant wistar rats OBJECTIVES OF THIS STUDY To examine the changes in serum low-density lipoprotein (LDL) cholesterol levels in pregnant wistar rats treated with Xylopia Aethiopica compared to untreated rats To assess the effect of Xylopia Aethiopica on the serum triglyceride levels in pregnant Wistar rats 5
- 7. EXPERIMENTAL DESIGN STUDY AREA Thisstudy was carried out in the animal house of the Department of Anatomy, Faculty of Basic Medical Sciences, University of Benin, Benin City. ANIMAL DESIGN Sixty (60) female young Wistar rats of comparable age weighing between 130g to 140g were purchased from the animal house of the Department of Anatomy, where the experiment was carried out, the animals were allowed to acclimatized for a period of two weeks with free access to food and water before the commencement of the experiment, thereafter they are placed in partition cages with wire mesh floor 7
- 8. MATERIALS AND METHODOLOGY Dried fruits of xylopia aethiopica (pepper soup spices) Cages Growers mash feed Feeding plates and drinking bowls Plain sample bottles Dissecting set 5ml syringes and needles Chloroform Freshly prepared phosphate buffer solution Digital weighing balance Creamic pestle and mortar n-hexane (250g/1litre) light microscope 6
- 9. SAMPLE COLLECTION At theend of the experiment, the animals were sacrificed under chloroform anaesthesia and there after blood samples were collected through cardiac puncture as described by (D'armour et al.,1965). The blood samples were collected using syringes and transferred into plain sample bottles for analysis.
- 10. EXPERIMENTAL DESIGN GROUP A GROUPB GROUP C GROUP D GROUP E 8
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- 13. REFRENCES Moore, K. L.,Persaud, T. V. N., & Torchia, M. G. (2015). Before We Are Born: Essentials of Embryology and Birth Defects. Saunders 2(5):67-68. 8
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