This document discusses hematopoietic stem cells (HSCs), which give rise to other blood cells through hematopoiesis in the bone marrow. HSCs can be isolated from bone marrow, umbilical cord blood, and peripheral blood. They express surface markers like CD34, CD38, CD90, and CD133 that allow their detection. HSCs have applications in hematopoietic stem cell transplantation to treat patients after intensive chemotherapy damages their bone marrow. Other applications of stem cells discussed are for treating baldness, diabetes, and myocardial infarction.

1. Stem cells
04/09/2018 1
Yasmine Hosny
Teaching assistant-Faculty of Science-Ain Shams University
CELL Team Board Member
Yasmine Hosny Hassan

2. 1. Hematopoietic stem cells (HSCs).
2. Isolation of HSCs.
3. Sub-culturing and counting of stem cells.
4. Applications of stem cells.
Agenda …
04/09/2018 2Yasmine Hosny Hassan

3. Hematopoietic stem cells (HSCs)
04/09/2018 3
stem cells that give rise to other blood cells. This process
is called haematopoiesis. This process occurs in the red
bone marrow.
more than 500 billion blood cells are produced every day.
HSCs give rise to both the myeloid and lymphoid lineages
of blood cells.
Myeloid cells include monocytes, macrophages,
neutrophils, basophils, eosinophils, erythrocytes, and
megakaryocytes to platelets.
Lymphoid cells include T cells, B cells, and natural killer
cells. Yasmine Hosny Hassan

4. 04/09/2018 4Yasmine Hosny Hassan

5. Location
04/09/2018 5
Bone marrow
Umbilical Cord blood
Peripheral blood
Yasmine Hosny Hassan

6. Hematopoietic stem cells (HSCs)
04/09/2018 6Yasmine Hosny Hassan

7. 04/09/2018 Yasmine Hosny Hassan 7

8. 04/09/2018 8Yasmine Hosny Hassan
• https://drive.google.com/open?id=1CqvLj5ZU3qNo
EvIipz4Q1pr0uZyfWhcz
Cord blood collection

9. HSCs isolation
04/09/2018 9Yasmine Hosny Hassan

10. 04/09/2018 Yasmine Hosny Hassan 10
HSCs isolation
• https://drive.google.com/open?id=16DcGTSx 11-
O0 dY2YewbOditI2yJctZ-P

11. 04/09/2018 11Yasmine Hosny Hassan

12. Detection of HSCs
04/09/2018 12
The cultured HSCs express on their surface CD34,
CD38, CD90, CD133, CD105.
Yasmine Hosny Hassan

13. Subculture of stem cells
04/09/2018 13Yasmine Hosny Hassan

14. Counting stem cells
04/09/2018 14Yasmine Hosny Hassan

15. https://drive.google.com/open?id=1nSeT
PDX_4Figb3dUjLzRvnRXWdZMxUeX
04/09/2018 Yasmine Hosny Hassan 15
counting

16. Applications of stem cells
04/09/2018 Yasmine Hosny Hassan 16

17. 04/09/2018 17Yasmine Hosny Hassan

18. Applications
04/09/2018 18Yasmine Hosny Hassan

19. Hematopoietic stem cell transplant
Intense chemotherapy damages a patient’s bone
marrow where the stem cells for blood resides.
This left the patient vulnerable to infection, anemia
and bleeding.
These side effects of chemotherapy can be treated
with a bone marrow transplant.
04/09/2018 19Yasmine Hosny Hassan

20. 04/09/2018 20Yasmine Hosny Hassan

21. 04/09/2018 Yasmine Hosny Hassan 22
• https://drive.google.com/open?id=1awbUG4ZRgeIv
wQXze_cPUcEOWRT-sUZq
Baldness

22. stem cells and diabetes
04/09/2018 23Yasmine Hosny Hassan

23. Stem cells and myocardial infraction
04/09/2018 24Yasmine Hosny Hassan

24. Stem cells and myocardial infaraction
04/09/2018 25Yasmine Hosny Hassan

25. Stem cells and myocardial infaraction
04/09/2018 26Yasmine Hosny Hassan

26. 04/09/2018 27Yasmine Hosny Hassan

27. 04/09/2018 28Yasmine Hosny Hassan
• https://drive.google.com/open?id=11qxS-
Al2Qz72QqaMVR90UEtEgMWKI6sR
Heart

28. 04/09/2018 Yasmine Hosny Hassan 29

29. 04/09/2018 Yasmine Hosny Hassan 30

30. Thank you for
your attention!
Any Questions?
04/09/2018 31Yasmine Hosny Hassan