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EXPOSITORES:
CARDONA PINEDA SEBASTIAN JOSE
COLORADO MEJIA MARIA FERNANDA
TERCER SEMESTRE - MEDICINA
UPB
TRYPANOSOMA CRUZI
CHAGAS’ DISEASE
OVERALL
OBJECTIVE
Methods
DNA from T. cruzi and Other
Parasites.
Methods
Designing LAMP
Primers.
Methods
LAMP Assay for T. cruzi.
Methods
Detecting LAMP-T. cruzi Products.
RESULTS
DISCUSSION
AUTHOR ANALYSIS 😄/ 😞
Cita 24. A. Gonzalez, E. Prediger, M. E. Huecas, N.
Nogueira, and P. M. Lizardi, “Minichromosomal
repetitive DNA in Trypanosoma cruzi: its use in a high-
sensitivity parasite detection assay,” Proceedings of
the National Academy of Sciences of the United.
The author found that fraction 195b was the most
repeated fraction of trypanosoma cruzi and carried out
a series of tests to demonstrate if it had any type of
specificity in the parasite and therefore if it was useful
to detect the parasite.
😄
Cita 16. M. Piron, R. Fisa, N. Casamitjana et al.,
“Development of a real-time PCR assay for Trypanosoma
cruzi detection in blood samples,” Acta Tropica, vol. 103,
no. 3, pp. 195–200, 2007.
The objective of this study was to develop a real-time PCR
technique to detect Trypanosoma cruzi DNA in blood from
chagasic patients. Sensitivity and specificity tests were
carried out.
😄
Cita 38. E. Ghedin, F. Bringaud, J. Peterson et al.,
“Gene synteny and evolution of genome architecture
in trypanosomatids, ” Molecular and Biochemical
Parasitology, vol. 134, no. 2, pp. 183–191, 2004.
The authors compared the genetic code of 3 parasites that
commonly generate confusion due to the similarity of their
genes (trypanosomatid protozoa, Trypanosoma brucei,
Trypanosoma cruzi and Leishmania)
😄
CONCLUSIONS
SEMINARIO "A Trypanosoma cruzi Genome Tandem Repetitive Satellite DNA Sequence as a Molecular Marker for a LAMP Assay for Diagnosing Chagas’ Disease"
SEMINARIO "A Trypanosoma cruzi Genome Tandem Repetitive Satellite DNA Sequence as a Molecular Marker for a LAMP Assay for Diagnosing Chagas’ Disease"

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SEMINARIO "A Trypanosoma cruzi Genome Tandem Repetitive Satellite DNA Sequence as a Molecular Marker for a LAMP Assay for Diagnosing Chagas’ Disease"

  • 1. EXPOSITORES: CARDONA PINEDA SEBASTIAN JOSE COLORADO MEJIA MARIA FERNANDA TERCER SEMESTRE - MEDICINA UPB
  • 5. Methods DNA from T. cruzi and Other Parasites.
  • 7.
  • 11.
  • 12.
  • 13.
  • 14.
  • 15. DISCUSSION AUTHOR ANALYSIS 😄/ 😞 Cita 24. A. Gonzalez, E. Prediger, M. E. Huecas, N. Nogueira, and P. M. Lizardi, “Minichromosomal repetitive DNA in Trypanosoma cruzi: its use in a high- sensitivity parasite detection assay,” Proceedings of the National Academy of Sciences of the United. The author found that fraction 195b was the most repeated fraction of trypanosoma cruzi and carried out a series of tests to demonstrate if it had any type of specificity in the parasite and therefore if it was useful to detect the parasite. 😄 Cita 16. M. Piron, R. Fisa, N. Casamitjana et al., “Development of a real-time PCR assay for Trypanosoma cruzi detection in blood samples,” Acta Tropica, vol. 103, no. 3, pp. 195–200, 2007. The objective of this study was to develop a real-time PCR technique to detect Trypanosoma cruzi DNA in blood from chagasic patients. Sensitivity and specificity tests were carried out. 😄 Cita 38. E. Ghedin, F. Bringaud, J. Peterson et al., “Gene synteny and evolution of genome architecture in trypanosomatids, ” Molecular and Biochemical Parasitology, vol. 134, no. 2, pp. 183–191, 2004. The authors compared the genetic code of 3 parasites that commonly generate confusion due to the similarity of their genes (trypanosomatid protozoa, Trypanosoma brucei, Trypanosoma cruzi and Leishmania) 😄