This document presents a study on securing authentication in an Internet of Things (IoT)-based radio-frequency identification (RFID) system using the lightweight PRESENT block cipher algorithm. The PRESENT-256 cipher is used to mutually authenticate an RFID tag and reader in a two-stage process: tag recognition by the reader and mutual authentication between the tag and reader using PRESENT-80/128/256 cipher modules in electronic codebook mode. The authentication scheme is simulated using FPGA results verified with ChipScope tools. Resource usage is analyzed and found improved compared to existing PRESENT ciphers and lightweight algorithms. The secured authentication approach is also compared favorably to similar RFID mutual authentication methods.