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CURRICULUM VITAE
NAME: SAPTASHWA DATTA
DEGREE: B.TECH
CURRENT STATUS: 8TH SEMESTER, 4TH YEAR
MAJOR: GENETIC ENGINEERING
UNIVERSITY: SRM INSTITUTE OF SCIENCE AND TECHNOLGY
EDUCATION ___________________________________________________
CGPA: 7.51 (83.18%), MAJOR: GENETIC ENGINEERING, SRM UNIVERSITY, 2016-2020
PERCENTAGE: 85.2%, BRANCH: SCIENCE, AISSCE, BIRLA HIGH SCHOOL, 2016
CGPA: 9.2, CBSE, BIRLA HIGH SCHOOL, 2014
RESEARCH EXPERIENCE __________________________________________
1) 30th May to 30th June 2018
Short term project internship
Bio zone Research Pvt. Ltd.
Project description
Detection of resistance to antibiotics by disc diffusion assay on Escherichia coli sample isolated from urinary tract
infection patient. The gene responsible for conferring resistance to the Beta Lactam antibiotic was then screened using
PCR analysis. A project report was written in the form of a research paper
2) July 2018-till date Trainee and intern in a lab project
SRM Institute of Science and Technology
Guide: Dr. K.N. Rajnish (Associate professor)
Project description (Ongoing)
1)Recombinant expression of the pharmaceutical enzymes.
2) Cellulose synthesis by various bacteria
3)Comparative genomics of Serratia sp. (Undergraduate Thesis)
RESEARCH SKILLS __________________________________________
Techniques: Micropippeting, Basic Bacterial culture techniques Preparation of media, Agarose gel electrophoresis,
SDS –PAGE , Enzyme assays, Protein purification using affinity chromatography, Polymerase Chain reaction, Protein
estimation(Lowry’s method), Recombinant DNA technology in microbes,Congo Red Assay, Calcofluor assay, EPS
extraction, Crystal Violet Microtiter plate assay, Manual genome mining, Real- time PCR, Digital PCR, Genome
annotation, Pan Genome analysis using Galaxy, Cytoscape, STRING, Gene Mark
3) May 2019 – July 2019 Summer Research fellow
Center for BioSystems Science and Engineering (BSSE)
Indian Institute of Sciences (IISc)
Guide: Dr. Rachit Agarwal
Project description
Development of murine DMM model and an in-vitro secretome model for studying osteoarthritis
RESEARCH SKILLS __________________________________________
Techniques: Animal cell culture (C28/I2, HeLa), Histological studies (Tissue embedding, sectioning, Safranin-O
staining and imaging), Using Serological pipettes, Usage of Multiwell pipettes, FACS basics, Senescence induction
(BrdU and H2O2), ELISA, Immunocytochemistry, PLGA micro carrier formation and drug encapsulation by single
emulsion method, DIC microscopy basics, Confocal Microscopy basics, Light Microscopy
Achievements and Publications
1)Completed BEST summer research fellowship program at Center For Bio Systems Science And Engineering,
Indian Institute of Sciences, Bangalore (https://www.be.iisc.ac.in/BEST.html)
2)Single author review entitled “A systematic study on the recent crisis in public health in Kerala”. (Asian
Journal of Health Sciences (2347-5218)) https://doi.org/10.15419/ajhs.v5i1.444
3) First author review entitled “Metagenomic applications in microbial diversity, bioremediation, pollution
monitoring, enzyme and drug discovery” accepted in Environmental Chemistry letters (IF - 4.6)
Manuscripts in preparation/ Review
1) Research paper entitled “ Random UV mutagenesis for improvement of production of serratiopeptidase from
Serratia marcescens” submitted to the Journal of Pure and Applied Microbiology (Under Review)
2) Review paper entitled “ Enzyme Therapy: A forerunner in catalysing a healthy society” submitted to “ Expert
Opinion on Biological Therapy ( Revised and resubmitted ;Under review)
3) Research paper entitled “ Computational analysis of Drug resistance in Lactobacillus acidophilus ” (manuscript in
preparation)
Posters/ Papers presented
1) Presented a poster on “ Cellulose synthesis by Serratia marcescens : An Insilico approach” in “National
Conference on Make in India for the biotech way forward” organised by “Society of Chemical and Synthetic
Biology” on 30th and 31st August in Coimbatore, Tamil Nadu, India
MEMBERSHIPS/SOCIETIES
1) Student member of American Society for Biochemistry and Molecular Biology(ASBMB), United States of America
2) Student affiliate of Royal Society of Biology(RSB), United Kingdom
3) Student member of American Chemical Society (ACS), United States of America
4) Student member of Internationational Metabolic Engineering Society (IMES), part of AIChE, United States of
America
5) Student member of Society of Biological Engineers, part of AIChE, United States of America
References
1) Dr. KN Rajnish (Associate Professor , SRMIST )
Contact details
Phone: +91 9445373014
Email: rajnish.n@ktr.srmuniv.ac.in, knrmku@gmail.com
Dr. Selvarajan Ethiraj ( Research Assistant Professor, SRMIST )
Contact details
Phone: +91 9843430245
Email: selvarajan.e@srmuniv.ktr.ac.in, selrajan@gmail.com

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Saptashwa cv

  • 1. CURRICULUM VITAE NAME: SAPTASHWA DATTA DEGREE: B.TECH CURRENT STATUS: 8TH SEMESTER, 4TH YEAR MAJOR: GENETIC ENGINEERING UNIVERSITY: SRM INSTITUTE OF SCIENCE AND TECHNOLGY EDUCATION ___________________________________________________ CGPA: 7.51 (83.18%), MAJOR: GENETIC ENGINEERING, SRM UNIVERSITY, 2016-2020 PERCENTAGE: 85.2%, BRANCH: SCIENCE, AISSCE, BIRLA HIGH SCHOOL, 2016 CGPA: 9.2, CBSE, BIRLA HIGH SCHOOL, 2014 RESEARCH EXPERIENCE __________________________________________ 1) 30th May to 30th June 2018 Short term project internship Bio zone Research Pvt. Ltd. Project description Detection of resistance to antibiotics by disc diffusion assay on Escherichia coli sample isolated from urinary tract infection patient. The gene responsible for conferring resistance to the Beta Lactam antibiotic was then screened using PCR analysis. A project report was written in the form of a research paper 2) July 2018-till date Trainee and intern in a lab project SRM Institute of Science and Technology Guide: Dr. K.N. Rajnish (Associate professor) Project description (Ongoing) 1)Recombinant expression of the pharmaceutical enzymes. 2) Cellulose synthesis by various bacteria 3)Comparative genomics of Serratia sp. (Undergraduate Thesis) RESEARCH SKILLS __________________________________________ Techniques: Micropippeting, Basic Bacterial culture techniques Preparation of media, Agarose gel electrophoresis, SDS –PAGE , Enzyme assays, Protein purification using affinity chromatography, Polymerase Chain reaction, Protein estimation(Lowry’s method), Recombinant DNA technology in microbes,Congo Red Assay, Calcofluor assay, EPS extraction, Crystal Violet Microtiter plate assay, Manual genome mining, Real- time PCR, Digital PCR, Genome annotation, Pan Genome analysis using Galaxy, Cytoscape, STRING, Gene Mark
  • 2. 3) May 2019 – July 2019 Summer Research fellow Center for BioSystems Science and Engineering (BSSE) Indian Institute of Sciences (IISc) Guide: Dr. Rachit Agarwal Project description Development of murine DMM model and an in-vitro secretome model for studying osteoarthritis RESEARCH SKILLS __________________________________________ Techniques: Animal cell culture (C28/I2, HeLa), Histological studies (Tissue embedding, sectioning, Safranin-O staining and imaging), Using Serological pipettes, Usage of Multiwell pipettes, FACS basics, Senescence induction (BrdU and H2O2), ELISA, Immunocytochemistry, PLGA micro carrier formation and drug encapsulation by single emulsion method, DIC microscopy basics, Confocal Microscopy basics, Light Microscopy Achievements and Publications 1)Completed BEST summer research fellowship program at Center For Bio Systems Science And Engineering, Indian Institute of Sciences, Bangalore (https://www.be.iisc.ac.in/BEST.html) 2)Single author review entitled “A systematic study on the recent crisis in public health in Kerala”. (Asian Journal of Health Sciences (2347-5218)) https://doi.org/10.15419/ajhs.v5i1.444 3) First author review entitled “Metagenomic applications in microbial diversity, bioremediation, pollution monitoring, enzyme and drug discovery” accepted in Environmental Chemistry letters (IF - 4.6) Manuscripts in preparation/ Review 1) Research paper entitled “ Random UV mutagenesis for improvement of production of serratiopeptidase from Serratia marcescens” submitted to the Journal of Pure and Applied Microbiology (Under Review) 2) Review paper entitled “ Enzyme Therapy: A forerunner in catalysing a healthy society” submitted to “ Expert Opinion on Biological Therapy ( Revised and resubmitted ;Under review) 3) Research paper entitled “ Computational analysis of Drug resistance in Lactobacillus acidophilus ” (manuscript in preparation) Posters/ Papers presented 1) Presented a poster on “ Cellulose synthesis by Serratia marcescens : An Insilico approach” in “National Conference on Make in India for the biotech way forward” organised by “Society of Chemical and Synthetic Biology” on 30th and 31st August in Coimbatore, Tamil Nadu, India MEMBERSHIPS/SOCIETIES 1) Student member of American Society for Biochemistry and Molecular Biology(ASBMB), United States of America 2) Student affiliate of Royal Society of Biology(RSB), United Kingdom 3) Student member of American Chemical Society (ACS), United States of America 4) Student member of Internationational Metabolic Engineering Society (IMES), part of AIChE, United States of America 5) Student member of Society of Biological Engineers, part of AIChE, United States of America
  • 3. References 1) Dr. KN Rajnish (Associate Professor , SRMIST ) Contact details Phone: +91 9445373014 Email: rajnish.n@ktr.srmuniv.ac.in, knrmku@gmail.com Dr. Selvarajan Ethiraj ( Research Assistant Professor, SRMIST ) Contact details Phone: +91 9843430245 Email: selvarajan.e@srmuniv.ktr.ac.in, selrajan@gmail.com