The document lists products and specifications for a sandwich ELISA immunoassay to detect C-reactive protein (CRP). It includes an anti-CRP antibody produced in rabbit as the 1st antibody, human recombinant CRP as the antigen, and a goat anti-rabbit IgG secondary antibody labeled with Alexa Fluor 488 as the 2nd antibody. The antibodies and antigen are listed with their concentrations, storage temperatures, and product numbers from various suppliers like Sigma-Aldrich, BioVision, and Novus Biologicals. The assay involves immobilizing the 1st antibody, adding the CRP antigen, then detecting with the 2nd fluorescent antibody for measurement.
Canvax™ offers a wide range of high quality Human Recombinant Proteins for several research applications like ELISA, Western Blot, Antibody Production or Protein array.
Analysis of Aflatoxins in Pet Food by UHPLC Using PDA and Fluorescence DetectionPerkinElmer, Inc.
Commercially prepared pet foods are easy and economical ways to fulfill the nutritional requirements for pets. Dry pet food is produced with grains and cereal by-products rejected for human consumption. The contamination of these by-products, with toxigenic fungal metabolites called mycotoxins, pose a serious health threat to pets.
Aflatoxins, some of the most carcinogenic mycotoxins known, are classified as B1, B2, G1, and G2. Several aflatoxin outbreaks in commercial pet foods have been reported in the past few years. Symptoms from aflatoxin exposure include lethargy, anorexia, jaundice, and intravascular coagulation, the severity often varying based upon a pet’s breed, species, age, dose, length of exposure, and nutritional status. Even if affecting only a small percentage of commercial pet foods, problems with pet food safety impact the entire pet food industry due to recalls and loss of consumer loyalty. Such experiences have reaffirmed the need for commercial pet food manufacturers to devote extensive resources documenting product quality.
High capacity chromatography resin for the capture and purification of monoclonal antibodies
Tosoh Bioscience, a provider of chromatographic solutions for the isolation and analysis of biomolecules, introduced a new Protein A chromatography resin designed for the purification of monoclonal antibodies (mAbs). It is well-suited for high capacity capturing of immunoglobulin out of high titer feedstock. TOYOPEARL AF-rProtein A HC-650F achieves 30% to 50% greater antibody adsorption than similar products. It exhibits dynamic binding capacities of greater than 70 g/L at residence times of 5 minutes and greater than 50 g/L at residence times of 2 minutes with feedstock titers from 1 g/L to more than 10 g/L.
The recombinant ligand that is linked to the well proven methacrylic polymer backbone of TOYOPEARL media has been engineered to maintain capacity even after repeated exposure to alkaline solution. Its multipoint attachment to the TOYOPEARL matrix further enhances chemical and thermal stability. In practice this pays off for a low level of Protein A leaching and also for a high resistance to alkaline solutions applied in cleaning-in-place (CIP) procedures. Due to its rigid polymer matrix TOYOPEARL AF-rProtein A HC-650F also provides excellent pressure flow characteristics.
Protein A resins constitute a substantial cost in state-of-the-art mAb purification processes. Factors such as operating cycles, capacity, and mAb titer can have an impact on total costs associated with mAb purification. The high capacity of the new TOYOPEARL AF-rProtein A HC-650F resin and its high alkaline resistance increase product throughput, reduce operating costs and increase manufacturing productivity.
Over the past decade, there have been a growing number of mAb candidates entering the clinical pipeline. This results in a large increase on the demand for analytical characterization. This seminar discusses advances in analytical method development with analytical run times below 10 minutes for all routine methods with intelligent, integrated chromatography workflows. Orbitrap technology has been established as the most powerful MS technology for protein characterization. How this can be incorporated into a complete workflow for bio-pharma analysis is also discussed.
Canvax™ offers a wide range of high quality Human Recombinant Proteins for several research applications like ELISA, Western Blot, Antibody Production or Protein array.
Analysis of Aflatoxins in Pet Food by UHPLC Using PDA and Fluorescence DetectionPerkinElmer, Inc.
Commercially prepared pet foods are easy and economical ways to fulfill the nutritional requirements for pets. Dry pet food is produced with grains and cereal by-products rejected for human consumption. The contamination of these by-products, with toxigenic fungal metabolites called mycotoxins, pose a serious health threat to pets.
Aflatoxins, some of the most carcinogenic mycotoxins known, are classified as B1, B2, G1, and G2. Several aflatoxin outbreaks in commercial pet foods have been reported in the past few years. Symptoms from aflatoxin exposure include lethargy, anorexia, jaundice, and intravascular coagulation, the severity often varying based upon a pet’s breed, species, age, dose, length of exposure, and nutritional status. Even if affecting only a small percentage of commercial pet foods, problems with pet food safety impact the entire pet food industry due to recalls and loss of consumer loyalty. Such experiences have reaffirmed the need for commercial pet food manufacturers to devote extensive resources documenting product quality.
High capacity chromatography resin for the capture and purification of monoclonal antibodies
Tosoh Bioscience, a provider of chromatographic solutions for the isolation and analysis of biomolecules, introduced a new Protein A chromatography resin designed for the purification of monoclonal antibodies (mAbs). It is well-suited for high capacity capturing of immunoglobulin out of high titer feedstock. TOYOPEARL AF-rProtein A HC-650F achieves 30% to 50% greater antibody adsorption than similar products. It exhibits dynamic binding capacities of greater than 70 g/L at residence times of 5 minutes and greater than 50 g/L at residence times of 2 minutes with feedstock titers from 1 g/L to more than 10 g/L.
The recombinant ligand that is linked to the well proven methacrylic polymer backbone of TOYOPEARL media has been engineered to maintain capacity even after repeated exposure to alkaline solution. Its multipoint attachment to the TOYOPEARL matrix further enhances chemical and thermal stability. In practice this pays off for a low level of Protein A leaching and also for a high resistance to alkaline solutions applied in cleaning-in-place (CIP) procedures. Due to its rigid polymer matrix TOYOPEARL AF-rProtein A HC-650F also provides excellent pressure flow characteristics.
Protein A resins constitute a substantial cost in state-of-the-art mAb purification processes. Factors such as operating cycles, capacity, and mAb titer can have an impact on total costs associated with mAb purification. The high capacity of the new TOYOPEARL AF-rProtein A HC-650F resin and its high alkaline resistance increase product throughput, reduce operating costs and increase manufacturing productivity.
Over the past decade, there have been a growing number of mAb candidates entering the clinical pipeline. This results in a large increase on the demand for analytical characterization. This seminar discusses advances in analytical method development with analytical run times below 10 minutes for all routine methods with intelligent, integrated chromatography workflows. Orbitrap technology has been established as the most powerful MS technology for protein characterization. How this can be incorporated into a complete workflow for bio-pharma analysis is also discussed.
Cell biology is a branch of biology that studies the different structuresand functions of the cell and focuses mainly on the idea of the cell as the basic unit of life. Research in cell biology is closelyrelated to genetics, biochemistry, molecular biology, immunology, and developmental biology. Elabscience offers 1400+ antibodies for cell biology research, check now!
More antibodies:
http://www.elabscience.com/index.php/product/show/tid/73
It is easy to follow an established protocol recommended by the manufacturer. However that protocol need to be fine tuned for your lab to save money and time. If you have the luxury of time you may be able to save a bundle by staining using a very diluted antibody by staining overnight or even extending incubation time for one hour.
BILS 2015 Tosoh Bioscience
"Making the Impossible Possible – Chromatographic Solutions for Demanding Separations in Downstream Processing"
Judith Vajda, Regina Römling and Egbert Müller
Much research aims tounderstand the cardiovascular system during disease development with angiogenesis, vasculature, atherosclerosis and heart research. Elabscience has 1200+ Antibodies for cardiovascular research. Worth to check!
More antibodies click here：
http://www.elabscience.com/index.php/product/show/tid/73
Neuroscience is the scientific study of the nervous system. The scope of neuroscience has broadened to include different approaches used to study the molecular, cellular, developmental, structural, functional, evolutionary, computational, and medical aspects of the nervous system. Elabscience offers 380+ antibodies for your neuroscience research.
Click for more antibodies:
http://www.elabscience.com/index.php/product/show/tid/90
Temperature is used in reversed-phase HPLC to improve peak shape and efficiency for small MW compounds and is also beneficial in reducing analysis time, albeit at the cost of lowering selectivity. With proteins, peak shape in RPC is generally enhanced by parameters that stabilize a single denatured state. Temperature is one way that can dramatically affect the tertiary and quaternary structure of proteins, and thus a “denatured state”. As such, we investigated the impact of temperatures up to 90 ºC on RPC of several globular proteins and monoclonal antibodies (mAbs). While, as expected, retention decreased with increasing temperature, peak shape of most proteins was dramatically affected by temperature. However, when operated under optimal conditions, peak shape and resolution of antibodies on several commercial columns was superior on a 400 angstrom pore size BIOshell Fused-Core column. In general, under optimal conditions, the wide pore Fused-Core column exhibited narrower peak widths, higher peak heights, better resolution, and thus greater sensitivity.
April 2014 - Antibodies Newsflash - BBI SolutionsBBISolutions
This month’s newsflash focuses on our recombinant Fab antibodies. Find out more about their clinical relevance and see new performance data for Cystatin C, Myeloperoxidase and C Reactive Protein recombinant Fab antibodies.
Analysis of Bisphenol A in Toys by HPLC with Fluorescence DetectionPerkinElmer, Inc.
BPA is a chemical byproduct used in the production of plastics for its optical clarity and heat resistance properties. It has been shown to exhibit hormone-like properties, causing concerns over possible health risks. Since 2008, several governments have supported on-going studies to investigate its safety and to establish safe levels of exposure. In the U.S., the Food and Drug Administration (FDA) set the safe level to 5 mg/kg (by body weight) per day, while, in Europe, the European Food Safety Authority (EFSA) recently lowered its safe level to 4 μg/kg (by body weight) per day. As a result of the health concerns over human exposure to BPA, this compound is now closely monitored in specific products, including children’s goods.
HIV Vaccines Process Development & Manufacturing - Pitfalls & PossibilitiesKBI Biopharma
Originally presented at the HIV Vaccine Manufacturing Workshop –July 19th& 20th, 2017 by Abhinav A. Shukla, Ph.D.Senior Vice PresidentDevelopment & ManufacturingKBI Biopharma, Durham NC
Presentation of Fridtof Lund-Johansen in 1st International Antibody Validatio...St John's Laboratory Ltd
Fridtjof Lund-Johansen is an MDPhD who has worked for 30 years with antibodies and flow cytometry. He took his degree at the University of Bergen in Norway. He then did post-docs in California, first at Becton Dickinson in San Jose and then at DNAX instiute of Immunology in Palo Alto. He is now a PI at the Institute of Immunology at Oslo University Hospital where he leads an effort to develop antibody-based proteomics. His technology is called MAP for microsphere affinity proteomics. He will talk about how MAP can be used to test thousands of antibodies in parallel.
For more details about 1st international antibody validation forum please check on http://www.stjohnslabs.com/ac_cms/blog
Cell biology is a branch of biology that studies the different structuresand functions of the cell and focuses mainly on the idea of the cell as the basic unit of life. Research in cell biology is closelyrelated to genetics, biochemistry, molecular biology, immunology, and developmental biology. Elabscience offers 1400+ antibodies for cell biology research, check now!
More antibodies:
http://www.elabscience.com/index.php/product/show/tid/73
It is easy to follow an established protocol recommended by the manufacturer. However that protocol need to be fine tuned for your lab to save money and time. If you have the luxury of time you may be able to save a bundle by staining using a very diluted antibody by staining overnight or even extending incubation time for one hour.
BILS 2015 Tosoh Bioscience
"Making the Impossible Possible – Chromatographic Solutions for Demanding Separations in Downstream Processing"
Judith Vajda, Regina Römling and Egbert Müller
Much research aims tounderstand the cardiovascular system during disease development with angiogenesis, vasculature, atherosclerosis and heart research. Elabscience has 1200+ Antibodies for cardiovascular research. Worth to check!
More antibodies click here：
http://www.elabscience.com/index.php/product/show/tid/73
Neuroscience is the scientific study of the nervous system. The scope of neuroscience has broadened to include different approaches used to study the molecular, cellular, developmental, structural, functional, evolutionary, computational, and medical aspects of the nervous system. Elabscience offers 380+ antibodies for your neuroscience research.
Click for more antibodies:
http://www.elabscience.com/index.php/product/show/tid/90
Temperature is used in reversed-phase HPLC to improve peak shape and efficiency for small MW compounds and is also beneficial in reducing analysis time, albeit at the cost of lowering selectivity. With proteins, peak shape in RPC is generally enhanced by parameters that stabilize a single denatured state. Temperature is one way that can dramatically affect the tertiary and quaternary structure of proteins, and thus a “denatured state”. As such, we investigated the impact of temperatures up to 90 ºC on RPC of several globular proteins and monoclonal antibodies (mAbs). While, as expected, retention decreased with increasing temperature, peak shape of most proteins was dramatically affected by temperature. However, when operated under optimal conditions, peak shape and resolution of antibodies on several commercial columns was superior on a 400 angstrom pore size BIOshell Fused-Core column. In general, under optimal conditions, the wide pore Fused-Core column exhibited narrower peak widths, higher peak heights, better resolution, and thus greater sensitivity.
April 2014 - Antibodies Newsflash - BBI SolutionsBBISolutions
This month’s newsflash focuses on our recombinant Fab antibodies. Find out more about their clinical relevance and see new performance data for Cystatin C, Myeloperoxidase and C Reactive Protein recombinant Fab antibodies.
Analysis of Bisphenol A in Toys by HPLC with Fluorescence DetectionPerkinElmer, Inc.
BPA is a chemical byproduct used in the production of plastics for its optical clarity and heat resistance properties. It has been shown to exhibit hormone-like properties, causing concerns over possible health risks. Since 2008, several governments have supported on-going studies to investigate its safety and to establish safe levels of exposure. In the U.S., the Food and Drug Administration (FDA) set the safe level to 5 mg/kg (by body weight) per day, while, in Europe, the European Food Safety Authority (EFSA) recently lowered its safe level to 4 μg/kg (by body weight) per day. As a result of the health concerns over human exposure to BPA, this compound is now closely monitored in specific products, including children’s goods.
HIV Vaccines Process Development & Manufacturing - Pitfalls & PossibilitiesKBI Biopharma
Originally presented at the HIV Vaccine Manufacturing Workshop –July 19th& 20th, 2017 by Abhinav A. Shukla, Ph.D.Senior Vice PresidentDevelopment & ManufacturingKBI Biopharma, Durham NC
Presentation of Fridtof Lund-Johansen in 1st International Antibody Validatio...St John's Laboratory Ltd
Fridtjof Lund-Johansen is an MDPhD who has worked for 30 years with antibodies and flow cytometry. He took his degree at the University of Bergen in Norway. He then did post-docs in California, first at Becton Dickinson in San Jose and then at DNAX instiute of Immunology in Palo Alto. He is now a PI at the Institute of Immunology at Oslo University Hospital where he leads an effort to develop antibody-based proteomics. His technology is called MAP for microsphere affinity proteomics. He will talk about how MAP can be used to test thousands of antibodies in parallel.
For more details about 1st international antibody validation forum please check on http://www.stjohnslabs.com/ac_cms/blog
Personal Brand Statement:
As an Army veteran dedicated to lifelong learning, I bring a disciplined, strategic mindset to my pursuits. I am constantly expanding my knowledge to innovate and lead effectively. My journey is driven by a commitment to excellence, and to make a meaningful impact in the world.
India Orthopedic Devices Market: Unlocking Growth Secrets, Trends and Develop...Kumar Satyam
According to TechSci Research report, “India Orthopedic Devices Market -Industry Size, Share, Trends, Competition Forecast & Opportunities, 2030”, the India Orthopedic Devices Market stood at USD 1,280.54 Million in 2024 and is anticipated to grow with a CAGR of 7.84% in the forecast period, 2026-2030F. The India Orthopedic Devices Market is being driven by several factors. The most prominent ones include an increase in the elderly population, who are more prone to orthopedic conditions such as osteoporosis and arthritis. Moreover, the rise in sports injuries and road accidents are also contributing to the demand for orthopedic devices. Advances in technology and the introduction of innovative implants and prosthetics have further propelled the market growth. Additionally, government initiatives aimed at improving healthcare infrastructure and the increasing prevalence of lifestyle diseases have led to an upward trend in orthopedic surgeries, thereby fueling the market demand for these devices.
3.0 Project 2_ Developing My Brand Identity Kit.pptxtanyjahb
A personal brand exploration presentation summarizes an individual's unique qualities and goals, covering strengths, values, passions, and target audience. It helps individuals understand what makes them stand out, their desired image, and how they aim to achieve it.
What are the main advantages of using HR recruiter services.pdfHumanResourceDimensi1
HR recruiter services offer top talents to companies according to their specific needs. They handle all recruitment tasks from job posting to onboarding and help companies concentrate on their business growth. With their expertise and years of experience, they streamline the hiring process and save time and resources for the company.
Enterprise Excellence is Inclusive Excellence.pdfKaiNexus
Enterprise excellence and inclusive excellence are closely linked, and real-world challenges have shown that both are essential to the success of any organization. To achieve enterprise excellence, organizations must focus on improving their operations and processes while creating an inclusive environment that engages everyone. In this interactive session, the facilitator will highlight commonly established business practices and how they limit our ability to engage everyone every day. More importantly, though, participants will likely gain increased awareness of what we can do differently to maximize enterprise excellence through deliberate inclusion.
What is Enterprise Excellence?
Enterprise Excellence is a holistic approach that's aimed at achieving world-class performance across all aspects of the organization.
What might I learn?
A way to engage all in creating Inclusive Excellence. Lessons from the US military and their parallels to the story of Harry Potter. How belt systems and CI teams can destroy inclusive practices. How leadership language invites people to the party. There are three things leaders can do to engage everyone every day: maximizing psychological safety to create environments where folks learn, contribute, and challenge the status quo.
Who might benefit? Anyone and everyone leading folks from the shop floor to top floor.
Dr. William Harvey is a seasoned Operations Leader with extensive experience in chemical processing, manufacturing, and operations management. At Michelman, he currently oversees multiple sites, leading teams in strategic planning and coaching/practicing continuous improvement. William is set to start his eighth year of teaching at the University of Cincinnati where he teaches marketing, finance, and management. William holds various certifications in change management, quality, leadership, operational excellence, team building, and DiSC, among others.
Putting the SPARK into Virtual Training.pptxCynthia Clay
This 60-minute webinar, sponsored by Adobe, was delivered for the Training Mag Network. It explored the five elements of SPARK: Storytelling, Purpose, Action, Relationships, and Kudos. Knowing how to tell a well-structured story is key to building long-term memory. Stating a clear purpose that doesn't take away from the discovery learning process is critical. Ensuring that people move from theory to practical application is imperative. Creating strong social learning is the key to commitment and engagement. Validating and affirming participants' comments is the way to create a positive learning environment.
Accpac to QuickBooks Conversion Navigating the Transition with Online Account...PaulBryant58
This article provides a comprehensive guide on how to
effectively manage the convert Accpac to QuickBooks , with a particular focus on utilizing online accounting services to streamline the process.
Affordable Stationery Printing Services in Jaipur | Navpack n PrintNavpack & Print
Looking for professional printing services in Jaipur? Navpack n Print offers high-quality and affordable stationery printing for all your business needs. Stand out with custom stationery designs and fast turnaround times. Contact us today for a quote!
Explore our most comprehensive guide on lookback analysis at SafePaaS, covering access governance and how it can transform modern ERP audits. Browse now!
Unveiling the Secrets How Does Generative AI Work.pdfSam H
At its core, generative artificial intelligence relies on the concept of generative models, which serve as engines that churn out entirely new data resembling their training data. It is like a sculptor who has studied so many forms found in nature and then uses this knowledge to create sculptures from his imagination that have never been seen before anywhere else. If taken to cyberspace, gans work almost the same way.
Premium MEAN Stack Development Solutions for Modern BusinessesSynapseIndia
Stay ahead of the curve with our premium MEAN Stack Development Solutions. Our expert developers utilize MongoDB, Express.js, AngularJS, and Node.js to create modern and responsive web applications. Trust us for cutting-edge solutions that drive your business growth and success.
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Cracking the Workplace Discipline Code Main.pptxWorkforce Group
Cultivating and maintaining discipline within teams is a critical differentiator for successful organisations.
Forward-thinking leaders and business managers understand the impact that discipline has on organisational success. A disciplined workforce operates with clarity, focus, and a shared understanding of expectations, ultimately driving better results, optimising productivity, and facilitating seamless collaboration.
Although discipline is not a one-size-fits-all approach, it can help create a work environment that encourages personal growth and accountability rather than solely relying on punitive measures.
In this deck, you will learn the significance of workplace discipline for organisational success. You’ll also learn
• Four (4) workplace discipline methods you should consider
• The best and most practical approach to implementing workplace discipline.
• Three (3) key tips to maintain a disciplined workplace.
1. Name Concentration
Cysteamine
10 mM
Antibody (Ab) Troponin I
10 g/ml
Blocking solution BSA
2 mg/ml
Antigen (5 kind of samples) 1;2;3;4 and blank sample
Secondary Ab Troponin I @ Alexa-488
2 g/ml
15 samples: Glass /Cr (2 nm)/Ag (50 nm)/Au (2nm)
immunoassay reaction
2. Glass
surface
COOR
Ab estradiol
(90 mins-40C)
O
O
O
3% BSA in PBS
30 mins Estradiol antigen
(30 mins)
O
O
O
O
O
O
Washing PBS Secondary Ab @ Alexa-488
(Incubation 2 hrs-27 0C)
O
O
O
Measurement
Washing PBS
Immobilization Estradiol on Glass surface
Washing PBS
3. COOR
Cysteamine
(2 hrs) Ab estradiol
(90 mins-40C)
Estradiol antigen
(30 mins)
Secondary Ab @ Alexa-488
(Incubation 2 hrs-270C)
Au
surface
3% BSA in PBS
30 mins
Washing PBS
Washing PBS
Immobilization Estradiol on Au surface
Washing PBS
4. COOR
Cysteamine
(2 hrs) Ab estradiol
(90 mins-40C)
Estradiol antigen
(30 mins)
Secondary Ab @ Alexa-488
(Incubation 2 hrs-40 0C)
Au
surface
3% BSA in PBS
30 mins
Washing PBS
Washing PBS
Immobilization estradiol on Au surface
Washing PBS
5. Name of product Concentration
Antibody (Ab) estradiol 10 ug/ml
Estradiol samples from E-WON 10 serum samples
Secondary antibody (Ab) estradiol
@ [Alexa Fluor 488]
0.53 ug/ml
(Diluted 500 times from original second Ab
@Alexa)
Estradiol
10 serum samples Secondary antibody (Ab2) estradiol
@ [Alexa Fluor 488]
Antibody (Ab) estradiol
1 2 3 4 5
6 7 8 9 10
6. No. Model number Name of product Price Company
1 ab215528
http://www.abcam.com/17-beta-estradiol-
antibody-epptx-r2-ab215528.html
Anti-17-beta-Estradiol
antibody [EPPTX-R2]
Host species: Rabbit
Abcam
2 ab120657
http://www.abcam.com/17beta-estradiol-
ab120657.html
Distributor in Korea:
Dawinbio Inc.
601, 150, Jojeong-daero
Hanam-city, Gyeonggi-do,
12930
Seoul, Rep of Korea
Tel: +82-15889741 Fax: +82-
317904360 Emaildawinbio@dawinbio.com
Websitehttp://www.dawinbio.com
17β-Estradiol Abcam
3 A-11008
https://www.thermofisher.com/antibody/product/G
oat-anti-Rabbit-IgG-H-L-Cross-Adsorbed-
Secondary-Antibody-Polyclonal/A-11008
Distributor in Korea:
써모 피셔 사이언티픽 코리아 주식회사
대표자 : 석수진
서울시 강남구 영동대로 302(대치동, 국민제1빌딩
6층)
대표번호 : 02-3420-8600
사업자 등록번호 : 117-81-46910
장비수리 관련 문의 : 1661-5055
Goat anti-Rabbit IgG
(H+L) Cross-Adsorbed
Secondary Antibody,
Alexa Fluor 488
238,000 krw Thermofisher
7. No. Model number Name of product Price Company
1 ab215528
http://www.abcam.com/17-beta-estradiol-
antibody-epptx-r2-ab215528.html
Distributor in Korea:
Dawinbio Inc.
601, 150, Jojeong-daero
Hanam-city, Gyeonggi-do,
12930
Seoul, Rep of Korea
Tel: +82-15889741 Fax: +82-
317904360 Emaildawinbio@dawinbio.com
Websitehttp://www.dawinbio.com
Anti-17-beta-Estradiol
antibody [EPPTX-R2]
Host species: Rabbit
Abcam
2 A-11008
https://www.thermofisher.com/antibody/product/G
oat-anti-Rabbit-IgG-H-L-Cross-Adsorbed-
Secondary-Antibody-Polyclonal/A-11008
Distributor in Korea:
써모 피셔 사이언티픽 코리아 주식회사
대표자 : 석수진
서울시 강남구 영동대로 302(대치동, 국민제1빌딩
6층)
대표번호 : 02-3420-8600
사업자 등록번호 : 117-81-46910
장비수리 관련 문의 : 1661-5055
Goat anti-Rabbit IgG
(H+L) Cross-Adsorbed
Secondary Antibody,
Alexa Fluor 488
238,000 krw Thermofisher
13. COOR
Cysteamine
(2 hrs) Ab estradiol
(90 mins-40C)
Estradiol antigen
(30 mins)
Secondary Ab @ Alexa-488
(Incubation 2 hrs-40 0C)
Au
surface
3% BSA in PBS
30 mins
Washing PBS
Washing PBS
Immobilization estradiol on Au surface
Washing PBS
14. Glass
surface
COOR
Ab estradiol
(90 mins-40C)
O
O
O
3% BSA in PBS
30 mins Estradiol antigen
(30 mins)
O
O
O
O
O
O
Washing PBS Secondary Ab @ Alexa-488
(Incubation 2 hrs-40 0C)
O
O
O
Measurement
Washing PBS
Immobilization Estradiol on Glass surface
Washing PBS
O2 plasma +
APTES
15. COOR
Anti-C-Reactive Protein antibody
produced in rabbit
COOH
+ EDC + NHS
COOR
BSA blocking solution
C-reactive/CRP human
C-Reactive Protein/CRP Antibody (5A9)
[Alexa Fluor® 488]
Cysteamine
Anti-C-Reactive
Protein antibody
produced in rabbit
C-reactive/CRP
human
C-Reactive Protein/CRP
Antibody (5A9)
[Alexa Fluor® 488]
Au
surface
Method 0
16. COOR
COOH
+ EDC + NHS
COOR
BSA blocking solution
C-reactive/CRP human
Cysteamine C-reactive/CRP
human
Au
surface
Method 1
1st ab CRP in PBS
1st ab CRP in PBS
17. COOR
2nd ab Goat anti-Rabbit in 10 mM acetate
buffer
COOH
+ EDC + NHS
COOR
BSA blocking solution
Cysteamine
2nd ab Goat anti-
Rabbit in 10 mM
acetate buffer
free unbound antibodies
Au
surface
subsequently
deactivated
1 M ethanolamine
Unreacted
25 ug/mL
+ CRP
1st ab CRP in PBS
Incubated in rotating
1 h at RT
free unbound
antibodies
1st ab CRP in PBS
Method 2
anti-E. coliO157:H7 polyclonal antibody
solution diluted in PBS, -> mixed with 300 μL of
E. coliO157:H7-> incubated in rotating for 1 h->
centrifugation process for 2 min-> free
unbound antibodies
18. No. Model number Name of product Price Company
1 C3527-1VL
https://www.sigmaaldrich.com/catalog/product/sigma/c
3527?lang=ko®ion=KR&gclid=Cj0KCQjwnqzWBRC
_ARIsABSMVTNAAGxxfryZYzmTO2L1vnocT8R7wBZI
43MrwuS2hFOIk555TlQh5N8aAkhEEALw_wcB
Anti-C-Reactive Protein
antibody produced in
rabbit
1st
482,000
krw
Sigma-
Aldrich
1st
2 7242
https://www.biovision.com/human-cellexptm-c-
reactive-crp-human-recombinant.html
Human CellExp™ C-
reactive/CRP, human
recombinant
150 $ BioVision
5 https://www.thermofisher.com/antibody/product/Goat-
anti-Rabbit-IgG-H-L-Secondary-Antibody-
Oligoclonal/A27034
Goat anti-Rabbit IgG
(H+L) Superclonal™
Secondary Antibody,
Alexa Fluor 488
344 kw 2nd
(Note anti-
Rabbit)
Không có
thông tin của
Gene
Final-Buy
CRP
19. No. Model number Name of product Price Company
1 C3527-1VL
https://www.sigmaaldrich.com/catalog/product/sigma/c
3527?lang=ko®ion=KR&gclid=Cj0KCQjwnqzWBRC
_ARIsABSMVTNAAGxxfryZYzmTO2L1vnocT8R7wBZI
43MrwuS2hFOIk555TlQh5N8aAkhEEALw_wcB
Anti-C-Reactive Protein
antibody produced in
rabbit
1st
482,000 krw Sigma-
Aldrich
1st
2 7242
https://www.biovision.com/human-cellexptm-c-
reactive-crp-human-recombinant.html
Distributor in Korea: Yes
Human CellExp™ C-
reactive/CRP, human
recombinant
150 $ BioVisio
n
3 NBP1-04282AF488
https://www.novusbio.com/products/c-reactive-protein-
crp-antibody-5a9_nbp1-04282af488
Distributor in Korea:
Seoulin Bioscience Co., Ltd.
Phone: +82-1670-5911
Fax: +82-31-628-3008
Email: antibody@antibodycenter.co.kr
WOONGBEE MeDiTech, Inc.
Phone: 82 2 881 5432
Fax: 82 2 881 5454
Email: woongbee@woongbee.com
C-Reactive Protein/CRP
Antibody (5A9) [Alexa
Fluor® 488]
(0.1ml)
1st
(no price in
the website)
Novus
Biologicals
1st
4 https://www.thermofisher.com/antibody/product/Goat-
anti-Rabbit-IgG-H-L-Secondary-Antibody-
Polyclonal/31460
Goat anti-Rabbit IgG
(H+L) Secondary
Antibody, HRP dye
(horseradish peroxidase)
258kw
HRP dye ???
2nd
5 https://www.thermofisher.com/antibody/product/Goat-
anti-Rabbit-IgG-H-L-Secondary-Antibody-
Oligoclonal/A27034
Goat anti-Rabbit IgG
(H+L) Superclonal™
Secondary Antibody,
Alexa Fluor 488
344 kw 2nd
23. No. Model number Name of product Price Company
1 C3527-1VL
https://www.sigmaaldrich.com/catalog/product/sigma/c
3527?lang=ko®ion=KR&gclid=Cj0KCQjwnqzWBRC
_ARIsABSMVTNAAGxxfryZYzmTO2L1vnocT8R7wBZI
43MrwuS2hFOIk555TlQh5N8aAkhEEALw_wcB
Anti-C-Reactive Protein
antibody produced in
rabbit
Store at: 2-8 oC
(15 mg/ml)
482,000 krw Sigma-
Aldrich
2 7242
https://www.biovision.com/human-cellexptm-c-reactive-
crp-human-recombinant.html
Human CellExp™ C-
reactive/CRP, human
recombinant
(200 ug/ml)
Store at: -20 oC
150 $ BioVision
3 NBP1-04282AF488
https://www.novusbio.com/products/c-reactive-protein-
crp-antibody-5a9_nbp1-04282af488
C-Reactive Protein/CRP
Antibody (5A9) [Alexa
Fluor® 488]
(0.1ml - 0.54 mg/ml)
Store at: 4 oC
(no price in
the website)
Novus
Biologicals
Name Concentration
Cysteamine
10 mM
Antibody 1
10 g/ml
Blocking solution BSA
2 mg/ml
Antigen (concentration of samples) ----
Antibody 2- Alexa Fluor® 488
2 g/ml
24. Name of product Name of product
Anti-C-Reactive Protein antibody produced in rabbit
Store at: 2-8 oC
(15 mg/ml)
Anti-C-Reactive Protein antibody produced in rabbit
Store at: 2-8 oC
(15 mg/ml)
Human CellExp™ C-reactive/CRP, human recombinant
(200 ug/ml)
Store at: -20 oC
Human CellExp™ C-reactive/CRP, human
recombinant
(200 ug/ml)
Store at: -20 oC
C-Reactive Protein/CRP Antibody (5A9) [Alexa Fluor® 488]
(0.1ml - 0.54 mg/ml)
Store at: 4 oC
C-Reactive Protein/CRP Antibody (5A9) [Alexa
Fluor® 488]
(0.1ml - 0.54 mg/ml)
Store at: 4 oC
C-Reactive Protein
30. No. Model number Name of product Price Company
1 ab215528
http://www.abcam.com/17-beta-estradiol-
antibody-epptx-r2-ab215528.html
Anti-17-beta-Estradiol
antibody [EPPTX-R2]
Host species: Rabbit
Abcam
2 ab120657
http://www.abcam.com/17beta-estradiol-
ab120657.html
Distributor in Korea:
Dawinbio Inc.
601, 150, Jojeong-daero
Hanam-city, Gyeonggi-do,
12930
Seoul, Rep of Korea
Tel: +82-15889741 Fax: +82-
317904360 Emaildawinbio@dawinbio.com
Websitehttp://www.dawinbio.com
17β-Estradiol Abcam
3 A-11008
https://www.thermofisher.com/antibody/product/G
oat-anti-Rabbit-IgG-H-L-Cross-Adsorbed-
Secondary-Antibody-Polyclonal/A-11008
Distributor in Korea:
써모 피셔 사이언티픽 코리아 주식회사
대표자 : 석수진
서울시 강남구 영동대로 302(대치동, 국민제1빌딩
6층)
대표번호 : 02-3420-8600
사업자 등록번호 : 117-81-46910
장비수리 관련 문의 : 1661-5055
Goat anti-Rabbit IgG
(H+L) Cross-Adsorbed
Secondary Antibody,
Alexa Fluor 488
238,000 krw Thermofisher
Estradiol
31. N-Hydroxysuccinimide(NHS)
1-(3-Dimethylaminopropyl)−3- ethalcarbodimide hydrochloride(EDC)
Phosphate buffer saline(PBS)
Bovine serum albumin (BSA)
for 10 min to create hydroxyl(-OH) groups on the surface (Baranowska et al.,
2015;Kim and Herr, 2013). The surfaces were then washed with distilled water.
Blocking to reduce subsequent non-specific binding was then performed by
incubating the sensor chip surface with a blocking solution (3% BSA in the PBS,
pH7.4) for 30 mins.
The biosensor chip was then washed with PBS for 5 mins. Subsequently,
functionalized fiber was incubated with cTnI antibody with concentration of
100μg/mL for 1 h to immobilize the antibody tofiber surface. Alongside, various
concentrations of cTnI (2 fg/mL to 10 fg/mL with a step of 2 fg/mL) proteins
solutions were prepared by dilution with PBS in advance.
33. Pha loãng 500/1000 lần:
Hũ 25 ug at 0.265 mg/mL
+ chiết ra 20 uL
+ Pha loãng 500 lần: 20 * 500=10.000
10 mL PBST (Tween 20)
+ Lấy 20 uL của hũ chính + 10 mL PBST
34. 2 mg/mL (có 0.5 ml) [m1 = V1 *C1] pha thành 2 µg/ml [m2 = V2 *C2]
Vtotal = 4000 uL (cho 15 samples) = VPBS + V
C=2ug/ml (nồng độ cần) => m=C.Vtotal = 4000 uL * 2mg/ml = 8ug
C1=2 mg/ml (nồng độ trong hũ) = 2000 ug/ml
=> V = m/C1 = 8 ug/ (2000 ug/ml) = 0.004 ml = 4 uL
V = 4 uL (lấy trong hũ dye mua)
=> VPBS = Vtotal - V = 4000 uL – 4 uL = 3996 uL (PBS có sẵn)
35. Species reactivity: Human (>>>)
Clonality
Monoclonal (dac hieu cao hon)
Species reactivity
Reacts with: Species independent
37. Anti-Cardiac Troponin I antibody
Anti-Cardiac Troponin I antibody (Alexa Fluor® 488-green color)
Recombinant Human Cardiac Troponin I protein (antigen)
Phân bố tủ lạnh
Estradiol
38. No. Model number Name of product Price Company
1 ab215528
http://www.abcam.com/17-beta-estradiol-
antibody-epptx-r2-ab215528.html
Anti-17-beta-Estradiol
antibody [EPPTX-R2]
Host species: Rabbit
Abcam
2 17β-Estradiol Abcam
3 A-11008
https://www.thermofisher.com/antibody/product/G
oat-anti-Rabbit-IgG-H-L-Cross-Adsorbed-
Secondary-Antibody-Polyclonal/A-11008
Distributor in Korea:
써모 피셔 사이언티픽 코리아 주식회사
대표자 : 석수진
서울시 강남구 영동대로 302(대치동, 국민제1빌딩
6층)
대표번호 : 02-3420-8600
사업자 등록번호 : 117-81-46910
장비수리 관련 문의 : 1661-5055
Goat anti-Rabbit IgG
(H+L) Cross-Adsorbed
Secondary Antibody,
Alexa Fluor 488
238,000 krw Thermofisher
AFP
Sửa
39. Albumin fraction V (BSA), heat shock
isolation pH 7.0
http://www.biosesang.com/bbs/shop/item.ph
p?it_id=A1025
40. Cần cùng 2mg/mL
Pha: 200 mn BSA + 100 mL PBS
Trữ 4C
30 min nhúng mẫu vào dung dịch
