1. Role Summary
1st Role:Designanddevelopmentof protocolsandprocessesforhighthrough-putautomation;
Biochemical advisortoRoboticsandAutomationDevelopmentGroupandPurchasingDepartment;
Headof the SangerInstitute AutomationandProcessesAdvisoryCommittee. Memberof the Stores
and PurchasingUserGroup; Productionsequencingprotocol trouble shooter;New commercial
productevaluatorandco-developer.External:Memberof the ScientificCommitteesforthe
EuropeanLaboratoryRoboticsInterestGroup. ScientificChairforLabAutomationEurope;
ConsultantforCorningLife Sciences,Millipore LifeScience,WhatmanInternational,PerkinElmer
liquidhandlingdivision,Edge BioSystems. Referee forJournal of the AssociationforLaboratory
Automation.
Achievementsunpublished:
In house development:
Designof highthroughputautomatedproductionpipelines
Ongoingreductioninsequencingcostsandimproveddataquality(1/2to1/256 enzyme volume,
20µl to 1µl reactionvolume,sequence lengthfrom550 to 750 bases, 96 well handpreppingto96
and 384 well automatedprepping,96well sequencingto384 well sequencing,introductionof PE
MiniTrakautomationsystems).
Designof nanolitre sequencingprotocols
Designof sequencingdilutionbuffers
Introductionof GS20 system
96 and 384 re-transformationmethodologies
Commercial productdevelopment:
Designof Millipore,WhatmanandCorning384 well filterplates.
Designof protocolsforhighthroughputprocessingof Agencourt,Invitogen,EdgeBiobeadclean-up
systems
Designof Whatmanand Pall blottingpapersequencingclean-up
Consultantonthe designof the G-stormthermocycler.
Designof Thermodeepwell 384well box filler.
2nd Role:Manage highthroughputgenotypingandsequencingteam.Developandmanage
automatedprocessesandprotocolstoanalyse recombinantvectorsandgenotype EScells.
Lecturing/Teaching:Wellcome TrustAdvance Course - EScell.
Management:SeniorResearchAssistants(1) andResearchAssistants(7) anddatainput.

2. Commercial productdevelopment: SequalPrepLRPCRkit(Invitrogen)