PTSD AND NEUROGENESIS Post-traumatic stress disorder (PTSD) is a stress-related mental illness that can develop after exposure to trauma. The hypothesis is that new neuron formation (neurogenesis) in the hippocampus is important for recovering from PTSD symptoms. The study used transgenic rats to examine the effects of stress on neurogenesis and behavior. Rats underwent a single prolonged stress protocol to induce PTSD-like symptoms. They were then tested on elevated plus maze and novelty suppressed feeding tests. Results showed stressed rats with inhibited neurogenesis had increased anxiety-like behavior and reduced volume in the ventral region of the hippocampus compared to other rats. The conclusion is ongoing neurogenesis is critical for recovery

1. PTSD AND NEUROGENESIS
Submitted by,
Arghya Chowdhury
18162027
M.Pharm 2nd semester
Submitted to,
Dr. Vinod Tiwari
Assistant Professor
Department of pharmaceutical engineering and technology, IIT(BHU),Varanasi

2. What is it
• A stress-related mental disease which can
occur after exposure to serious traumatic
events.
• If untreated, can continue for years.
• 300,000 Number of veterans of Iraq
and Afghanistan wars affected.
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3. Present Scenario
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4. Signs and symptoms
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5. Hypothesis
• New neurons(neurogenesis) are important for
recovery of normal behavior and hippocampal
structure altered in PTSD.
• The ventral CA1 region as a potential key
mediator of stress-induced anxiety-like behavior.
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6. Experimental animals
• Transgenic male rats expressing Herpes Simplex Virus
Thymidine Kinase (HSV-TK) .
• VGCV (Valganciclovir) was administered (4 mg/rat) to all WT
and TK rats twice weekly starting at 16 weeks of age prior to
stress and continued until the animals were perfused.
• 12 hr light–dark cycle maintained.
• Followed the Institute of Laboratory Animal Research
guidelines.
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7. Experimental design
• Experiment 1
• WT and TK rats underwent SPS at 16 weeks of age .
• n = 8–9
• Underwent the tests (EPM, NSF) beginning 9 days after stress.
• All rats were perfused 31 days following stress .
• Experiment 2
• WT and TK rats underwent SPS at 16 weeks of age .
• n = 10–11
• Underwent the tests (EPM, NSF) beginning 36 days after stress.
• All rats were perfused 58 days following stress .
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8. Induction of PTSD
• Single prolonged stress (SPS)
Clear plexiglass
restraint tubes
for 2 hr
Cold water swim
(10°C) for 5 min
Home cages for
15 min
Exposed to
Isoflurane
until unconscious
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9. Tests and assessments
• Elevated plus-maze (EPM)
• Novelty-suppressed feeding(NSF)
• Volume analysis of Dorsal and
ventral CA1
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10. Results :
EPM
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11. Results :
NSF
*p < 0.05 compared to control or WT 11

12. Results :
ventral CA1
volume
*p < 0.05 compared to control or WT 12

13. CONCLUSION
• Circuit-based approaches suggest that ventral CA1
regions of hippocampus are uniquely involved in anxiety-
like behavior in rodents.
• This research suggest a progression of susceptibility to
stress-induced volumetric decrease from ventral CA1 and
also demonstrated ongoing neurogenesis in the dentate
gyrus is critical for recovery of ventral CA1 volume and
associated anxiety-like behaviors in PTSD .
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14. REFERENCE
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15. Don’t worry; be happy
Thanks you