1) The document summarizes research optimizing the amplification of three osteoblast gene markers (BMP2, OPN, and RUNX2) from human stem cells in exfoliated deciduous teeth (SHED) using reverse transcriptase PCR (RT-PCR). 2) Through several trials adjusting annealing temperature, time, and other PCR parameters, the researchers obtained a single clear band at the expected size on agarose gel for each gene marker. 3) The optimum PCR conditions identified for each gene were: BMP2 at 61.9°C, OPN at 63.2°C, and RUNX2 at 63.6°C.