This study investigated the role of the neuritin (NRN) protein in dendritic spine morphology in striatal medium spiny neurons (MSNs). MSNs were cultured and infected at DIV2 with a virus expressing shRNA to knock down NRN or a control virus. Dendritic spines on GFP/DARPP32 stained cells were analyzed at DIV11-19. Spine density and maturation were quantified and compared between wild-type and NRN knockdown neurons. No significant differences were found, suggesting NRN may not play a role in MSN spine development within the first 19 days in culture. However, a trend of increasing spine density and maturation over time was observed.