PolyGen GmbH is a leading developer and manufacturer of DNA synthesizers. They produce basic 10 column, professional 12 column, industrial 96 column, and custom 384 column DNA synthesizers. The synthesizers use a patented slider block system for efficient synthesis. PolyGen also provides software, service and training to support their DNA synthesizers.

1. The specialists in DNA synthesis
Company
Portfolio

2. The specialists in DNA synthesis
About us
• PolyGen GmbH is an international renowned supplier of DNA
synthesizers for biomedical sciences.
• Since 1990 PolyGen is one of the leading developers and
manufacturers of DNA synthesizers for the production of
Oligonucleotides.
• PolyGen distributes its products and services in Germany through sales
and marketing directly, and via selected specialized dealers worldwide.
• Due to intensive product development Oligonucleotides are successfully
being synthesized throughout the world with PolyGen synthesizers.
• In intensive cooperation with universities and research institutions
PolyGen’s product range is constantly being optimized.
This dialogue with other institutions helps us to define our goals and
leads to product expansion.

3. The specialists in DNA synthesis
• Your products are always as good as the people
behind them…..
• Roland Friedberger: Managing director
has for more than 20 years experience as a
specialist in DNA synthesis, he has been building
his own synthesizers since 1990. He is responsible
for sales and post sales handling.
• Ursula Friedberger: F&A, Purchase
Accounting and Finance are her assignment. She
is responsible for shipment, invoicing, finance and
administration.
The Team

4. The specialists in DNA synthesis
Production & Service
Test phase new main boards
Assembling of 10-column Synthesizers
Space for hands on and service training
96-column Synthesizers quality control
Conference and meeting room

5. The specialists in DNA synthesis
Mechanical parts production in Langen
The mechanical parts are manufactured in
Langen, Germany. The manufacturing and
assembling of the synthesizers are done in
the same building. Short ways helps us to
communicate fast and directly. This makes
working flexible and efficient.
Software Development in Mühlheim
The AKC team develops the software and manufactures the electronic
hardware for the synthesizers in Mühlheim which is close to Langen. They
are specialized in communication between synthesizers and PC software.
Production and
manufacturing in one place
Polygen GmbH, Germany

6. The specialists in DNA synthesis
Worldwide Partners
For all other countries and Germany:
PolyGen GmbH; Mr. Roland Friedberger
Karlstr. 10, 63225 Langen, Germany
Metis Biyoteknoloji ve Dis Tic. Ltd. Sti.
Mr. Serdar Tuncer, Alinteri Bulvari Ostim
Is Merkezi, C Blok No :17 Ostim,
06370 Ankara, Turkyie
NOOR SCIENTIFIC & TRADE
Mr. Mostafa Ismail,10 El-Salam
Street,Kornish El-Nile Aghakhan, Shoubra,
Cairo - 11241 Egypt
MIIL MEDI-SPEC INSTRUMENTS
INDIA PRIVATE LIMITED,
Mr. Vippul Chhatbar, Unit 201,
Prarmeshwar Industrial Estate, Plot Cts
1406/9, Chincholi Bunder Road, Malad
West, MUMBAI 400 064 India
BOSUNG Scientific CO.,LTD.
Mr. Manbok Lee, #408 Kolon Digital
Tower, 222-7 Guro3-dong, Guro-gu,
Seoul, 152-777, Korea
BIOGENOMED CO.,LTD.
80/1 Suthisarnvinitchai Rd.,
Samsennok, Huay Kwang
Bangkok 10320, Thailand 10320
MEDICONCEPTS HEALTHCARE (P) LTD
Contact Person : Anshu Sood
170, Saini Enclave, Vikas Marg
New Delhi-110092 INDIA

7. The specialists in DNA synthesis
The Products
DNA Synthesizers
Software
Service

8. The specialists in DNA synthesis
Product Lines
•Basic 10 column instruments
•Professional 12 column instruments
•Industrial 96 column Workstation
384 column SynTower®

9. The specialists in DNA synthesis
The workhorse
Standard system for routine synthesis. Up to 10 different Oligonucleotides
can be synthesized parallel, up to 80 Oligonucleotides (20mer) within 24
hours.
Flexible
4 standard Amidite positions, two spare
Amidite positions can be used as premixing
wobbles or modifiers
Easy
Synthesis can also be done without
a PC system
Small and compact
Instrument size 43 x 39 x 36 cm (LxDxH)
BASIC Line
10 column DNA Synthesizer

10. The specialists in DNA synthesis
• Exact measurement
of reagent addition via integrated metering pump
• Variable flow rates
and times in each individual program step
• Wide synthesis range
from 10 nmole - 5 µmole per column.
• Short synthesis times
10 couplings in 6 minutes - 10 Oligonucleotide syntheses in one run.
20 Poly-T in 1h 45 min in the fast program and up to 2 h 55 min in
the high quality program.
• Saving maintenance/service costs
No expensive service/ and maintenance contracts. Many services
can be taken over by the user, maintenance free slider block system.
Features and benefits

11. The specialists in DNA synthesis
• Direct transfer from and to the PC
of sequences, programs, protocols from your
database to the synthesizer.
• Operation as stand-alone instrument or via pc.
Recommended software: PolyEasy.
• DMT system for reliable process monitoring
DMT monitoring for each column, for constant
process control with stop or warning function.
• High degree of user-friendliness
No valve replacement required. Easy to maintain.
• Flexible, user-oriented software
Various software suites are available.
• Special Design
At customer's wish case can be varnished
with individual design.
Features and benefits

12. The specialists in DNA synthesis
Patented Slider Block
Extremely economical in reagent consumption
due to the patented slider block with low dead volume (~3 µl)
0.6 – 1,2 ml total solvent consumption per coupling
(depending on program)
Saving costs
no use of expensive pre-packed
columns, the CPG are directly fed
into the column slider.

13. The specialists in DNA synthesis
The flexible
Optimal design for modifying oligomers, 20% higher throughput: up to 12
different oligomers can be synthesized parallel, up to 96 oligomers
(20mer) within 24 hours.
Professional
8 Amidite positions allows you to use
various modifications 3 additional inlets
for reagents. Suitable for creating RNA,
LNA, premixed wobbles
Minimal space requirement
The compact design 41 x 43 x 40 cm
(LxDxH) means that a small area only is required.
Professional line –
12 column DNA Synthesizer

14. The specialists in DNA synthesis
Features and benefits
• Minimal reagent consumption
extremely economical due to the patented slider
block of low dead volume (ca. 3 µl). In the fast program: only 0.6 ml
total solvent consumption per coupling. In the high-quality program:
only 1.2 ml total solvent consumption per coupling. Measurement of
reagent addition is done by integrated metering pump.
• Variable flow rates
and times in each individual program step
• Wide synthesis range
from 10 nmole - 5 µmole per column.
• DMT system
for reliable process monitoring

15. The specialists in DNA synthesis
• Short synthesis times
12 couplings in 6 minutes - 12 Oligo syntheses in one run. 20-unit Poly-
T in 1h 45 min in the fast program and up to 2 h 55 min in the high-
quality program.
• High degree of user-friendliness and ease of maintenance
No valve replacement is required. Maintenance-free due to patented
slider block system. Service and maintenance contracts unnecessary.
• Direct data transfer from your PC
The 12-Column DNA Synthesizer can be operated as a stand-alone
instrument or with various software suites via PC.
• Thus the direct transfer of sequences from your
database into the synthesizer is possible.
Various software suites are available.
Features and benefits

16. The specialists in DNA synthesis
• Individual process control
Each individual column can be monitored by DMT; the entire synthesis
process is thus constantly under review. If the minimum required
absorption is not attained, a warning signal is given; alternatively
process can be stopped.
• No costly consumables
Pre-packed columns become unnecessary as the reaction vessels are
integrated in the slider block system. The CPG materials are directly
fed into the column slider.
• Your own casing
upon customer’s request housings can be
varnished with individual designs.
Features and benefits

17. The specialists in DNA synthesis
Industrial Line
96-Column DNA Synthesis Workstation

18. The specialists in DNA synthesis
Features and benefits
• High range
1 – 96 Oligonucleotides; the DNA Synthesis Workstation enables up to
1000 Oligonucleotides (20mer) to be synthesized within 24 h.
• Independent control
the different modules can be controlled via separate power supplies
and processor circuit boards. In case of occurring errors, one module
can be stopped or serviced while the others continue to synthesize.
• Flexible
The modules can be run separately with different synthesis programs
and at different scales.
• Convenient placement in your lab
The required space is minimal: 120 x 80 x 100 cm
(length x depth x height).

19. The specialists in DNA synthesis
Features and benefits
• Calculation tools
Calculation and monitoring of reagent consumption is possible. The
entire DNA Synthesis Workstation can be provided with reagents from
a central source and the consumption monitored.
The reagents are supplied from large-volume reservoirs that can be
customized to the synthesis scale of users. In this way frequent refilling
of reagents is rendered unnecessary.
• Reliable process monitoring
Separate DMT monitoring for all 96 columns.
The module function is based on the patented slider system, as is used
in the proven 10-column synthesizer.

20. The specialists in DNA synthesis
384-Column
DNA-SynTower®
Custom designed on your demands and wishes

21. The specialists in DNA synthesis
Features and benefits
• 4000 Oligonucleotides a day
The DNA SynTower® enables up to 4000 Oligonucleotides (20mer)
to be synthesized within 24 h
• Modular design
The DNA SynTower® is the latest development of the PolyGen
instrument line. Its modular design is based on the DNA Synthesis
Workstation.
• Independent operation
Up to 32 modules with 12 columns each can be operated
independently. Thus, up to 384 Oligonucleotides can be synthesized in
parallel.

22. The specialists in DNA synthesis
Features and benefits
• Flexible working conditions
Independent control of the different modules via separate power
supplies and processor circuit boards. Should errors occur modules
can be stopped or serviced while the others continue to synthesize.
The modules can be run separately with different synthesis programs
and at different scales.
• Reliable process monitoring
Separate DMT monitoring for all 96 columns. The module function is
based on the patented slider system, as is used
in the proven 10-column synthesizer.
• Convenient placement in your lab
The required space is minimal: 120 x 120 x 100 cm
(LxDxH).

23. The specialists in DNA synthesis
Features and benefits
• Calculation and monitoring of reagent consumption
The entire DNA Synthesis Workstation can be provided with reagents
from a central source and the consumption can be monitored. The
reagents are supplied from large-volume reservoirs that can be
customized to the synthesis scale of users. In this way frequent refilling
of reagents is rendered unnecessary.

24. The specialists in DNA synthesis
Software
• POLYEasy
• Polygen Synthese Manager

25. The specialists in DNA synthesis
POLYEasy
• Controlling the 10 column synthesizer
Up to 30 DNA Synthesizers can be controlled simultaneously.
You can communicate with the appliance via RS 232, RS 485
or Ethernet interface or a combination of these.
New and older instruments
can be connected.

26. The specialists in DNA synthesis
Modifier Database
• Modifier specific coupling programs on your
demands and needs, free programmable
• Wobbles (mixed sites) database already
implemented
• Standard or universal CPG possible

27. The specialists in DNA synthesis
Synthesis programs
• Setting, saving, selection and import of your own synthesis
programs (e.g. of 16-bit software*)
• Saving of any number of synthesis programs
*Will soon be replaced with the PolyEasy Software.

28. The specialists in DNA synthesis
Sequence entry
• Sequences can be preset, saved, selected and imported
• Saving of any number of sequences
• Sequence database with search and filter functions

29. The specialists in DNA synthesis
DMT monitoring
• DMT monitoring allows online quality control of the
single coupling steps.
• Absorption measurement of Trityle group separation
via photometric detection.
• Warning level function in case of low absorption values
• Automatic stop function
• Graphic illustration of DMT
values as a column diagram
• Automatic display of DMT
values on the various columns

30. The specialists in DNA synthesis
Firmware Update Manager
• Simple updating with new firmware via serial interface

31. The specialists in DNA synthesis
PolyGen Synthese
Manager
• Professional solution (high-end) for
12-Column DNA Synthesizer
96-Column DNA Synthesis Workstation
384-Column DNA SynTower®

32. The specialists in DNA synthesis
• This software has been developed for controlling the workstation
(with 12-column modules).
• If several 10-Column DNA synthesizers are to be used in parallel, all
can be controlled and administered by the software.
• The instruments can be used individually or in combination with the
Polygen Synthese Manager Software.
• Software adaptation
acc. to customer requirements possible at any time
• Multi-user facility
allows different access rights for the users
• Microsoft SQL Server database
includes MSDE; license-free version limited to 5 users
• PolyGen communications software
(in background)
Individual - combined

33. The specialists in DNA synthesis
Features and benefits
• Update Manager for Firmware
allows you to update the firmware easily, as soon as the new releases
are available
• PolyGen synthesizer service software
simple and fast service maintenance tools implemented in the
instrument software allows you to check or recalibrate your instrument
and to detect faults and failures
• Communication set-up software for networks
Easy set-up and implementation of instruments and software for your
network system
• Customer data administration software
all contact information for you administration can
be entered into a data base.

34. The specialists in DNA synthesis
more features …
• Reagent administration
Calculation of reagent consumption, reagent storage program
• Documentation
GMP and GLP- compatible documentation for the reagents used
• Procedure records
Protocols of used syntheses programs and used reagents
• Numerous import functions
e.g. orders, sequences from the database, Excel, e-mails, websites...
• Modifier management
Phosphorthioates, Wobbles (premixing);
the various modifiers can be allocated to
specific coupling programs.
• RNA standard protocols and programs
available on request

35. The specialists in DNA synthesis
Controlling the
Workstation
• Form generator for labels
(incl. barcode) and printouts, barcode readers may be used
• Simple and rapid setting, importing and allocation of synthesis
programs and sequences.
• Multi-programming
Up to three different synthesis programs selectable for one run
• Autoprime, prime and auto print functions
• DMT monitoring (Trityle monitoring with minimum level alarm)
- and much more.

36. The specialists in DNA synthesis
Services
• Training on-site / PolyGen inhouse training
• Installation
• Spare Parts
• Routine maintenance/repair
• Software and instrument updates and upgrades
• References

37. The specialists in DNA synthesis
In-house or On-site
• Training
• Service
• Parts
• Software
• Hardware
• Handling
• Installations
• Repair
• Routine maintenance
• Spare parts
• Replacements
• Updates and Upgrades

38. The specialists in DNA synthesis
Training
The standard training session during installation takes …
• For 10 and 12 columns synthesizers and software
3 days training on instrument and software handling
• For 96 and 384 columns synthesizers and software
5 days training on instrument and software handling
• Special training sessions on request
according to your demands – in-house or on-site
or in partner labs

39. The specialists in DNA synthesis
Service
The service and maintenance is reduced to a minimum, due to:
• User friendliness
Users can handle the maintenance by themselves, boards, pumps
and sliders can easily be replaced.
• Saving costs and time
No expensive service contracts have to be fulfilled, this
reduces costs and saves time.
• Device maintenance
Instruments can be shipped to PolyGen for intensive repairs,
minimizing travelling expenditure and manpower costs.

40. The specialists in DNA synthesis
Parts
• Ordering
All parts can be ordered via our partners or Polygen, directly. They
can be shipped via express service, to minimize the down time. A set
of the most important spare parts (for ~two years) will be delivered
with the instrument. Reordering after use keeps your spare parts
stock always up to date and minimizes down times.
• Replacements/Upgrades
New parts are downwards compatible with older (starting blue
PolyGen logo) instruments. This allows you to upgrade your
instruments and to keep them up to date.
• Software/Firmware updates www.polygen.de
All software and firmware updates can be downloaded from the
PolyGen homepage. In case of no access or slow internet connection,
contact us via fax, E-mail or post to get it free of charge.

41. The specialists in DNA synthesis
Contact
PolyGen GmbH
Mr. Roland Friedberger
Managing Director
Karlstr. 10
63225 Langen
Germany, Europe
Homepage: www.polygen.de
E-mail: info@polygen.de
phone: +49.6103.55559
fax: +49.6103.55461
Production:
Siemensstr. 4
63225 Langen
Germany, Europe