1. MicroRNA-613 represses lipogenesis in HepG2 cells
by downregulating LXRalpha
AND
The specifity of urinary aquaporin 1 and perilipin 2
to screen for renal cell carcinoma
MATEO ZAPATA NARANJO
UNIVERSIDAD PONTIFICIA BOLIVARIANA

4. INTRODUCTION
In these times when medicine has been evolving
and every day shows the importance of
research, molecular biology techniques and
especially their transfer of genetic material have
reached places as accurate and enabled evaluate
everything. Most of the techniques used have
shown an essence on the basics, as many
people had forgotten that today are solving the
major problems of medicine

5. FIRST NEW
MicroRNA-613 represses
lipogenesis in HepG2 cells by
downregulating LXRalpha
The main objective was to
obtain a technique which
could regulate lipid
homeostasis by expression of
certain genes associated with
specific receptors tied to
microRNA's

6. FIRST NEW
The report showed that the microRNA miR-613
manages generally negatively regulate the liver
X receptor alpha, which is activated by a ligand
and is vital in the metabolism of lipids

7. FIRST NEW
This was performed in HepG2 cells, and all was
performed by real-time PCR, western blot and
luciferase. It was possible to demonstrate that
miR-613 induces formation of lipid droplets in
HepG2 cells and thereby down-regulating
receptor expression LXRalpha

8. FIRST NEW
In conclusion, was developed and obtained a
technique which is achieved by mediating the
metabolism of lipids, which is very important
from now on to treat all kinds of diseases
covering these compounds

9. FIRST NEW
My personal opinion on this article is that it is
very important what has been developed. Given
the conditions that cause lipid disorders, will be
very important in some way to correct these
issues in a manner based on the molecular. Each
day shows the importance of molecular biology
major alternatives pathologies.

10. SECOND NEW
The specifity of urinary
aquaporin 1 and perilipin 2 to
screen for renal cell carcinoma
This new shows us the
importance of diagnosing
quickly and easily renal
carcinoma. Have determined
concentrations of certain
metabolites in urine that
have come to be associated
with this disease.

11. SECOND NEW
Rapid diagnosis of renal cell carcinoma has not
been established by simple techniques. By AQP1
concentrations and PLIN2 that serve as
biomarkers in urine tests generate a light that is
associated with a rapid and simple diagnostic
allowing adequate and timely treatment

12. SECOND NEW
To reach such conclusions were
made several tests in people
with renal carcinoma and
several similar urinary
infections. AQP1 levels in urine
and PLIN2 were determined by
Western blotting. Very high
concentrations were found in
patients suffering from renal
carcinoma compared with other
diseases

13. SECOND NEW
In conclusion, the findings of these metabolites
help diagnose renal carcinoma and differentiate
it from other diseases that can cover something
more serious such as carcinoma. There will be
further assessed more results and comparing
them to establish the rapid diagnosis of renal
carcinoma.

14. SECOND NEW
In my opinion the development of the
mentioned mechanism is quite important in the
search for a diagnosis quickly above that allows
the doctor to easily interpret and act in an
appropriate manner. It was a first step but I
think quite appropriate to do a study of more
participants who are able to unify the concept
being treated and reach what has been
proposed

15. MEDICAL UTILITY
First New
The main medical benefit of this news is in
force that have taken the disease etiology in
lipid disorders and as mentioned technique
THROUGH modulate the effects are achieved
lipid own. It is important today for the diet
that currently contains the general population
to modify lipid metabolism and improve many
boxes each day disease claims the lives of
many people.

16. MEDICAL UTILITY
It is a situation, is a public health problem that is
truly useful to develop this technique.
Molecular biology greatly aid and generates
large amounts of expectations for development
that can result I think will be pretty good

17. MEDICAL UTILITY
Second New
The main use of this article
focuses on the speed and ease
with which the doctor can
diagnose a renal carcinoma
compared with other
procedures that generally do
not allow the diagnosis or that
take too long to adapt clinical
diagnosis for the real.

18. MEDICAL UTILITY
It is useful if this is accomplished medical check
since it would be a breakthrough in issues like
this that have lately been so unemployed and
revolutionize the approach to a simple urinary
tract infection.
The great use is accompanied by the ease and
results are obtained using a urine test that is
fairly common and that will bring the doctor to
the pathology

19. BIBLIOGRAPHY
• Martinez S. Lina María. Biología Molecular, 5 ed. Medellín,
UPB, Facultad de Medicina
• MicroRNA-613 represses lipogenesis in HepG2 cells by
downregulating LXRalpha, 7th Space interactive, March
8/2013,
http://7thspace.com/headlines/433798/microrna_613_repress
es_lipogenesis_in_hepg2_cells_by_downregulating_lxralpha.ht
ml
• The specifity of urinary aquaporin 1 and perilipin 2 to screen
for renal cell carcinoma, Uro Today, March 11/2013
http://www.urotoday.com/Renal-Cancer/the-specificity-of-
urinary-aquaporin-1-and-perilipin-2-to-screen-for-renal-cell-
carcinoma-abstract.html