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Plant Nutrition
The uptake of nutrients by a plant: an overview
Nutrient Uptake
in Plants
Using hydroponic culture to identify essential nutrients
Determination
of Nutrient
Needs
Essential elements
• Required to complete life cycle and
reproduction
• Macronutrients –needed in large amt
– Used to build carbs, proteins, lipids, nucleic acids
– C, H, O, N, P, S
• Micronutrients-needed in small amt
– Used in enzymatic rxns
– Cl, Fe, MN, B, Zn, Cu, Ni, Mo
Essential Nutrients in Plants
Nutrient Deficiency
• Has many effects, depends on tissue effected
• N, P, and K deficiencies most common
http://www.lsuagce
nter.com/NR/rdonly
res/9C41F5BD-
C699-43FE-809D-
064929A3744C/12
045/fertbg_w200.J
PG
Symtoms of Mineral Deficiency
Nutrient Mobility
• Leaf Color - Chlorosis
–Non-mobile nutrients – Chlorosis in
New Growth
• Iron
–Mobile nutrients – Chlorosis in Old
growth
• Magnesium
Healthy
Phosphate-deficient
Potassium-deficient
Nitrogen-deficient
Leaf Margin Necrosis in Poinsettia
Potassium (K) Deficiency
Macronutrient Deficiencies Beans
Blossom End Rot of
Tomato Calcium Deficiency
Right-Hydroponic tomatoes grown in the greenhouse, Left-Blossom end
rot of tomato fruits induced by calcium (Ca++) deficiency
Influence of Calcium on Root Induction
on Rose Cuttings
Magnesium Deficiency on Poinsettia
Interveinal Chlorosis on Mature Leaves
Iron Deficiency Symptoms
1 2
4
3
1-Piggyback Plant, 2- Petunia, 3-Silver Maple,
4-Rose (A-normal, B-Fe-deficient)
A B
Molybdenum Deficiency on Poinsettia
Micronutrient Toxicity on Seed Geranium
B
Cu
Fe
Mn
Mo
Zn
Concentration (mM)
Cont 0.25 0.5 1 2 3 4 5 6
Soil Texture & Composition
• Determined by size and amount of
particles
• Topsoil-mixture of rock, living org.,
and decaying organic matter (humus)
• Amount of sand, silt, and clay
determines soil type
Ions in soil
• Negative anions (NO3
-, H2PO4
- SO4
2-)
– Easily absorbed by plants
– Easily leached out of soil
• Positive cations (K+, Ca 2+, Mg 2+ )
– Not easily aborbed, use cation exchange
Soil particle surrounded by
film of water
Root hair
Water
available
to plant
Root hair
Air space
Cation exchange in soil
Soil water
Soil particle
H+ out, cation in
Nitrogen: A Special Nutrient
• Most important nutrient for growth
• Decomposition of Organic Matteer
• N2 (gas) must be converted to NH4+ or
NO3- in order for plants to use
Nitrogen: A Special Nutrient
•Nitrogen Fixation – Nitrogen Fixing Bacteria
•Convert N2 to NH3
•Nitrogenase
•Ammonium Ion – NH4
+
•Nitrate – NO3
- Produced in Soil by
Nitrifying Bacteria That Oxidize
Ammonium
•Plants Convert Nitrate to Ammonium
•Denitrifying Bacteria – Bad Guys
Atmosphere
Soil
Organic
material (humus)
N2
N2
Ammonifying
bacteria
NH4
+
(ammonium)
H+
(from soil)
NH3
(ammonia)
Nitrogen-fixing
bacteria
Soil
Atmosphere
Atmosphere
Soil
Organic
material (humus)
N2
N2
Ammonifying
bacteria
NH4
+
(ammonium)
H+
(from soil)
NH3
(ammonia)
Nitrogen-fixing
bacteria
Soil
Atmosphere
N2
Nitrifying
bacteria
NO3
–
(nitrate)
Denitrifying
bacteria
Root
Atmosphere
Soil
Organic
material (humus)
N2
N2
Ammonifying
bacteria
NH4
+
(ammonium)
H+
(from soil)
NH3
(ammonia)
Nitrogen-fixing
bacteria
Soil
Atmosphere
N2
Nitrifying
bacteria
NO3
–
(nitrate)
Denitrifying
bacteria
Root
NH4
+
Nitrate and
nitrogenous
organic
compounds
exported in
xylem to
shoot system
Mycorrhizae
•Fungus Root
•Increases Absorption of Water and
Nutrients, Particularly Phosphorus
•Ectomycorrhizae – 10% fungus on
outside of root hairs.
Increased water uptake in plants.
•Endomycorrhizae – 85% fungus on inside
of root hairs.
•Haustoria
•Arbuscules
•Agricultural Importance
Epidermis
Mantle
(fungal sheath)
(a) Ectomycorrhizae
Cortex Mantle
(fungal
sheath)
Endodermis
Fungal
hyphae
between
cortical
cells (colorized SEM)
100 µm
10 µm
Cortical cells
Endodermis
Fungal
vesicle
Casparian
strip
Arbuscules
Plasma
membrane (LM, stained specimen)
Cortex
Epidermis
Fungal
hyphae
Root
hair
(b) Arbuscular mycorrhizae (endomycorrhizae)
Nutritional adaptations
• Mutualism
– Legumes
• Nodules form w/nitrogen-fixing bacteria (Rhizobium)
• Species specific
• Used in crop rotation
– Mycorrhizae
• Fungus and roots together-inc. surface area
Bacteroids
within
vesicle
Nodules
Roots
Pea plant root. Bacteroids in a soybean
root nodule.
5 µm
Epidermis
Mantle
(fungal sheath)
Fungal
hyphae
between
cortical
cells
Endodermis
Mantle
(fungal
sheath)
Cortex
Ectomycorrhizae.
100 µm
(colorized SEM)
Nutritional adaptations
• Some plants have nutritional adaptations
that use other organisms in nonmutualistic
ways
Nutritional adaptations
• Epiphytes
– Plant nourishes self but grows on another
plant (orchids)
• Parasitic
– Absorb nutrients/sugars from host (mistletoe)
• Carnivorous
– Poor soils, get N by digesting insects, animals
(venus fly trap)
Parasitic Plants
•Mistletoe
•Dodder
•Indian Pipe
Carnivorous Plants
•Grow in bogs
•Acid soils
•Deficient in Nitrogen and Phosphorus
•Produce food by photosynthesis
•Secure Nitrogen and Phosphorus from
prey
http://www.netst
ate.com/states/
symb/flowers/i
mages/mistleto
e2.jpg
http://jrscience.w
cp.muohio.edu/P
hotos/Orchids.jp
eg

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Plant Nutrition and Nutrition Deficiency