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Chapter-12
MINEAL NUTRITION
Presented by
B.Prabhakar
Principal
JNV. Panchmahal (Guj)
Hydroponics
• In 1860, Julius von Sachs, a prominent
German botanist, demonstrated, for the
first time, that plants could be grown to
maturity in a defined nutrient solution
in complete absence of soil. This
technique of growing plants in a
nutrient solution is known as
hydroponics
12. Mineral Nutrition
The criteria for essentiality of an
element
• (A) the element must be absolutely
necessary for supporting normal growth and
reproduction. In the absence of the element
the plants do not complete their life cycle or
set the seeds.
• (B) the requirement of the element must be
specific and not replaceable by another
element. In other words, deficiency of any
one element cannot be met by supplying
some other element.
• (C) the element must be directly involved in
the metabolism of the plant.
Classification of essential elements
• Based upon the above criteria only a few elements have been found to
be absolutely essential for plant growth and metabolism. These
elements are further divided into two broad categories based on their
quantitative requirements
• (I) MACRONUTRIENTS, AND
(II) MICRONUTRIENTS
• Macronutrients are generally present in plant tissues in large amounts
(in excess of 10 mmole kg –1 of dry matter). The macronutrients
include carbon, hydrogen, oxygen, nitrogen, phosphorous, sulphur,
potassium, calcium and magnesium. Of these, carbon, hydrogen and
oxygen are mainly obtained from co2and h2o, while the others are
absorbed from the soil as mineral nutrition.
• Micronutrients or trace elements, are needed in very small amounts
(less than 10 mmole kg –1 of dry matter). These include iron,
manganese, copper, molybdenum, zinc, boron, chlorine and nickel.
• In addition to the 17 essential elements named above, there are some
beneficial elements such as sodium, silicon, cobalt and selenium.
They are required by higher plants.
Classification of essential elements
• Essential elements can also be grouped into four broad categories on the basis
of their diverse functions. These categories are:
• (I) essential elements as components of biomolecules and hence structural
elements of cells (e.G., Carbon, hydrogen, oxygen and nitrogen).
• (Ii) essential elements that are components of energy-related chemical
compounds in plants (e.G., Magnesium in chlorophyll and phosphorous in atp).
• (Iii) essential elements that activate or inhibit enzymes, for example mg2+ is an
activator for both ribulose bisphosphate carboxylaseoxygenase and
phosphoenol pyruvate carboxylase, both of which are critical enzymes in
photosynthetic carbon fixation; zn2+ is an activator of alcohol dehydrogenase
and mo of nitrogenase during nitrogen metabolism. Can you name a few more
elements that fall in this category? For this, you will need to recollect some of
the biochemical pathways you have studied earlier.
• (Iv) some essential elements can alter the osmotic potential of a cell. Potassium
plays an important role in the opening and closing of stomata. You may recall
the role of minerals as solutes in determining the water potential of a cell.
Nitrogen
• Nitrogen : this is the mineral element required by plants
in the greatest amount.
• It is absorbed mainly as No3– though some are also
taken up as No2 – or NH4 +.
• nitrogen is required by all parts of a plant, particularly
the meristematic tissues and the metabolically active
cells.
• Nitrogen is one of the major constituents of proteins,
nucleic acids, vitamins and hormones.
• Phosphorus: phosphorus is absorbed by the plants from
soil in the form of phosphate ions (either as h2po4− or
hpo42-). Phosphorus is a constituent of cell membranes,
certain proteins, all nucleic acids and nucleotides, and is
required for all phosphorylation reactions.
PHOSPHORUS:
• Phosphorus is absorbed by
the plants from soil in the form
of phosphate ions (either as
h2po4− or hpo42-).
• Phosphorus is a constituent of
cell membranes, certain
proteins, all nucleic acids and
nucleotides, and is required
for all phosphorylation
reactions.
Potassium
• it is absorbed as potassium ion (k+).
• In plants, this is required in more
abundant quantities in the meristematic
tissues, buds, leaves and root tips.
• Potassium helps to maintain an anion-
cation balance in cells and is involved in
protein synthesis, opening and closing of
stomata, activation of enzymes and in the
maintenance of the turgidity of cells.
CALCIUM
Plant absorbs calcium from the soil in the form of calcium
ions (ca2+).
Calcium is required by meristematic and differentiating
tissues.
During cell division it is used in the synthesis of cell wall,
particularly as calcium pectate in the middle lamella.
It is also needed during the formation of mitotic spindle.
It is involved in the normal functioning of the cell
membranes.
It activates certain enzymes and plays an important role in
regulating metabolic activities.
MAGNESIUM
• It is absorbed by plants in the form
of divalent mg2+.
• It activates the enzymes of
respiration, photosynthesis and are
involved in the synthesis of dna and
rna.
• Magnesium is a constituent of the
ring structure of chlorophyll and
helps to maintain the ribosome
structure.
• Manganese: it is absorbed in the form of manganous
ions (mn2+).
• It activates many enzymes involved in photosynthesis,
respiration and nitrogen metabolism.
• The best defined function of manganese is in the splitting
of water to liberate oxygen during hotosynthesis.
• Zinc: plants obtain zinc as zn2+ ions.
• It activates various enzymes, especially carboxylases.
• It is also needed in the synthesis of auxin.
• Copper: it is absorbed as cupric ions (cu2+).
• It is essential for the overall metabolism in
plants. Like iron,
• it is associated with certain enzymes involved in
redox reactions
• Boron: it is absorbed as Bo33− or B4o72−. Boron
is required for uptake and utilisation of ca2+,
membrane functioning, pollen germination, cell
elongation, cell differentiation and carbohydrate
translocation.
• Molybdenum: plants obtain it in the form of molybdate
ions (moo22-).
• It is a component of several enzymes, including
nitrogenase and nitrate reducta se both of which
participate in nitrogen metabolism.
• Chlorine: it is absorbed in the form of chloride anion
(cl–). Along with na+ and k+,
• it helps in determining the solute concentration and the
anioncation balance in cells.
• It is essential for the water-splitting reaction in
photosynthesis, a reaction that leads to oxygen evolution
Nitrogen cycle
• Nitrogen is a constituent of amino acids, proteins, hormones, chlorophylls and
many of the vitamins
• Nitrogen exists as two nitrogen atoms joined by a very strong triple covalent
bond (N ≡ N). The process of conversion of nitrogen (N2) to ammonia is termed
as nitrogen fixation. In nature, lightning and ultraviolet radiation provide enough
energy to convert nitrogen to nitrogen oxides (NO, NO2, N2O). Industrial
combustions, forest fires, automobile exhausts and power -generating stations
are also sources of atmospheric nitrogen oxides. Decomposition of organic
nitrogen of dead plants and animals into ammonia is called ammonification
• Most of it is converted into nitrate by soil bacteria in the following steps:
• 2NH3 + 3O2———–> 2NO2- + 2NH+ + 2H2O —-(I)
• 2NO2- + O2———–> 2NO3- —-(II)
• Ammonia is first oxidised to nitrite by the bacteria nitrosomonas and/or
nitrococcus. The nitrite is further oxidised to nitrate with the help of the
bacterium nitrobacter. These steps are called nitrification These nitrifying
bacteria are chemoautotrophs.
• The nitrate thus formed is absorbed by plants and is transported to the leaves. In
leaves, it is reduced to form ammonia that finally forms the amine group of amino
acids. Nitrate present in the soil is also reduced to nitrogen by the process of
denitrification. Denitrification is carried by bacteria pseudomonas and
thiobacillus.
12. Mineral Nutrition
Nitrogen fixation
• Certain prokaryotic species are capable of fixing nitrogen. Reduction
of nitrogen to ammonia by living organisms is called biological
nitrogen fixation. The enzyme, nitrogenase which is capable of
nitrogen reduction is present exclusively in prokaryotes. Such
microbes are called n2- fixers.
• The nitrogen-fixing microbes could be free-living or
symbiotic.
• Examples of free-living nitrogen-fixing aerobic microbes
are azotobacter and beijernickia
• Rodospirillum is anaerobic
• bacillus free-living, anaerobic
• cyanobacteria such as anabaena and nostoc are also
free-living nitrogen-fixers.
Symbiotic nitrogen fixation
• the legume-bacteria relationship.
Species of rod-shaped Rhizobium
has such relationship with the
roots of several legumes such as
alfalfa, sweet clover, sweet pea,
lentils, garden pea, broad bean,
clover beans
• The most common association on
roots is as nodules. These
nodules are small outgrowths on
the roots.
• The microbe, frankia, also
produces nitrogen-fixing nodules
on the roots of nonleguminous
plants (e.G., Alnus).
• Both rhizobium and frankia are
freeliving in soil, but as
symbionts, can fix atmospheric
nitrogen.
Root nodules
• Root nodules are
pink in colour.This is
due to the presence
of leguminous
haemoglobin or leg-
haemoglobin.
Nodule formation
• Nodule formation involves a sequence of multiple interactions between
rhizobium and roots of the host plant.
• 1.Rhizobia multiply and colonise the surroundings of roots and get
attached to epidermal and root hair cells.
• 2.The root-hairs curl and the bacteria invade the root-hair.
• 3,An infection thread is produced carrying the bacteria into the cortex
of the root, where they initiate the nodule formation in the cortex of the
root.
• 4.Then the bacteria are released from the thread into the cells which
leads to the differentiation of specialised nitrogen fixing cells.
• The nodule thus formed, establishes a direct vascular connection with
the host for exchange of nutrients
12. Mineral Nutrition
12. Mineral Nutrition
12. Mineral Nutrition
12. Mineral Nutrition
12. Mineral Nutrition
Nitrogenase
• The enzyme nitrogenase is highly
sensitive to the molecular oxygen; it
requires anaerobic conditions.
• the nodule contains an oxygen scavenger
called leg-haemoglobin
• N2 +8E- +8H+ +16ATP—–>2NH3 +H2
+16ADP+16P1
• the ammonia synthesis by nitrogenease
requires a very high input of energy (8
ATP for each NH3 produced). The energy
required, thus, is obtained from the
respiration of the host cells
FATE OF AMMONIA:
• At physiological ph, the ammonia is
protonated to form nh4+ (ammonium) ion.
While most of the plants can assimilate
nitrate as well as ammonium ions, the
latter is quite toxic to plants and hence
cannot accumulate in them. Let us now
see how the nh4+ is used to synthesise
amino acids in plants. There are two main
ways in which this can take place:
(i) Reductive amination :
• In these processes, ammonia reacts with
α-ketoglutaric acid and forms glutamic
acid
(ii) Transamination
• : It involves the transfer of amino group from one
amino acid to the keto group of a keto acid.
Glutamic acid is the main amino acid from which
the transfer of NH2, the amino group takes place
and other amino acids are formed through
transamination. The enzyme transaminase
catalyses all such reactions.
Amides
• The two most important amides – asparagine and
glutamine – found in plants are a structural part of
proteins.
• They are formed from two amino acids, namely aspartic
acid and glutamic acid, respectively, by addition of
another amino group to each. The hydroxyl part of the
acid is replaced by another nh2 – radicle.
• Since amides contain more nitrogen than the amino
acids, they are transported to other parts of the plant via
xylem vessels. In addition, along with the transpiration
stream
• the nodules of some plants (e.G., Soyabean) export the
fixed nitrogen as ureides. These compounds also have a
particularly high nitrogen to carbon ratio.

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11. Transport in Plants
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10. Cell Cycle and Cell Division
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9. Biomolecules
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6. anatomy of flowering plants
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7. structural organisation in animals
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12. Mineral Nutrition

  • 2. Hydroponics • In 1860, Julius von Sachs, a prominent German botanist, demonstrated, for the first time, that plants could be grown to maturity in a defined nutrient solution in complete absence of soil. This technique of growing plants in a nutrient solution is known as hydroponics
  • 4. The criteria for essentiality of an element • (A) the element must be absolutely necessary for supporting normal growth and reproduction. In the absence of the element the plants do not complete their life cycle or set the seeds. • (B) the requirement of the element must be specific and not replaceable by another element. In other words, deficiency of any one element cannot be met by supplying some other element. • (C) the element must be directly involved in the metabolism of the plant.
  • 5. Classification of essential elements • Based upon the above criteria only a few elements have been found to be absolutely essential for plant growth and metabolism. These elements are further divided into two broad categories based on their quantitative requirements • (I) MACRONUTRIENTS, AND (II) MICRONUTRIENTS • Macronutrients are generally present in plant tissues in large amounts (in excess of 10 mmole kg –1 of dry matter). The macronutrients include carbon, hydrogen, oxygen, nitrogen, phosphorous, sulphur, potassium, calcium and magnesium. Of these, carbon, hydrogen and oxygen are mainly obtained from co2and h2o, while the others are absorbed from the soil as mineral nutrition. • Micronutrients or trace elements, are needed in very small amounts (less than 10 mmole kg –1 of dry matter). These include iron, manganese, copper, molybdenum, zinc, boron, chlorine and nickel. • In addition to the 17 essential elements named above, there are some beneficial elements such as sodium, silicon, cobalt and selenium. They are required by higher plants.
  • 6. Classification of essential elements • Essential elements can also be grouped into four broad categories on the basis of their diverse functions. These categories are: • (I) essential elements as components of biomolecules and hence structural elements of cells (e.G., Carbon, hydrogen, oxygen and nitrogen). • (Ii) essential elements that are components of energy-related chemical compounds in plants (e.G., Magnesium in chlorophyll and phosphorous in atp). • (Iii) essential elements that activate or inhibit enzymes, for example mg2+ is an activator for both ribulose bisphosphate carboxylaseoxygenase and phosphoenol pyruvate carboxylase, both of which are critical enzymes in photosynthetic carbon fixation; zn2+ is an activator of alcohol dehydrogenase and mo of nitrogenase during nitrogen metabolism. Can you name a few more elements that fall in this category? For this, you will need to recollect some of the biochemical pathways you have studied earlier. • (Iv) some essential elements can alter the osmotic potential of a cell. Potassium plays an important role in the opening and closing of stomata. You may recall the role of minerals as solutes in determining the water potential of a cell.
  • 7. Nitrogen • Nitrogen : this is the mineral element required by plants in the greatest amount. • It is absorbed mainly as No3– though some are also taken up as No2 – or NH4 +. • nitrogen is required by all parts of a plant, particularly the meristematic tissues and the metabolically active cells. • Nitrogen is one of the major constituents of proteins, nucleic acids, vitamins and hormones. • Phosphorus: phosphorus is absorbed by the plants from soil in the form of phosphate ions (either as h2po4− or hpo42-). Phosphorus is a constituent of cell membranes, certain proteins, all nucleic acids and nucleotides, and is required for all phosphorylation reactions.
  • 8. PHOSPHORUS: • Phosphorus is absorbed by the plants from soil in the form of phosphate ions (either as h2po4− or hpo42-). • Phosphorus is a constituent of cell membranes, certain proteins, all nucleic acids and nucleotides, and is required for all phosphorylation reactions.
  • 9. Potassium • it is absorbed as potassium ion (k+). • In plants, this is required in more abundant quantities in the meristematic tissues, buds, leaves and root tips. • Potassium helps to maintain an anion- cation balance in cells and is involved in protein synthesis, opening and closing of stomata, activation of enzymes and in the maintenance of the turgidity of cells.
  • 10. CALCIUM Plant absorbs calcium from the soil in the form of calcium ions (ca2+). Calcium is required by meristematic and differentiating tissues. During cell division it is used in the synthesis of cell wall, particularly as calcium pectate in the middle lamella. It is also needed during the formation of mitotic spindle. It is involved in the normal functioning of the cell membranes. It activates certain enzymes and plays an important role in regulating metabolic activities.
  • 11. MAGNESIUM • It is absorbed by plants in the form of divalent mg2+. • It activates the enzymes of respiration, photosynthesis and are involved in the synthesis of dna and rna. • Magnesium is a constituent of the ring structure of chlorophyll and helps to maintain the ribosome structure.
  • 12. • Manganese: it is absorbed in the form of manganous ions (mn2+). • It activates many enzymes involved in photosynthesis, respiration and nitrogen metabolism. • The best defined function of manganese is in the splitting of water to liberate oxygen during hotosynthesis. • Zinc: plants obtain zinc as zn2+ ions. • It activates various enzymes, especially carboxylases. • It is also needed in the synthesis of auxin.
  • 13. • Copper: it is absorbed as cupric ions (cu2+). • It is essential for the overall metabolism in plants. Like iron, • it is associated with certain enzymes involved in redox reactions • Boron: it is absorbed as Bo33− or B4o72−. Boron is required for uptake and utilisation of ca2+, membrane functioning, pollen germination, cell elongation, cell differentiation and carbohydrate translocation.
  • 14. • Molybdenum: plants obtain it in the form of molybdate ions (moo22-). • It is a component of several enzymes, including nitrogenase and nitrate reducta se both of which participate in nitrogen metabolism. • Chlorine: it is absorbed in the form of chloride anion (cl–). Along with na+ and k+, • it helps in determining the solute concentration and the anioncation balance in cells. • It is essential for the water-splitting reaction in photosynthesis, a reaction that leads to oxygen evolution
  • 15. Nitrogen cycle • Nitrogen is a constituent of amino acids, proteins, hormones, chlorophylls and many of the vitamins • Nitrogen exists as two nitrogen atoms joined by a very strong triple covalent bond (N ≡ N). The process of conversion of nitrogen (N2) to ammonia is termed as nitrogen fixation. In nature, lightning and ultraviolet radiation provide enough energy to convert nitrogen to nitrogen oxides (NO, NO2, N2O). Industrial combustions, forest fires, automobile exhausts and power -generating stations are also sources of atmospheric nitrogen oxides. Decomposition of organic nitrogen of dead plants and animals into ammonia is called ammonification • Most of it is converted into nitrate by soil bacteria in the following steps: • 2NH3 + 3O2———–> 2NO2- + 2NH+ + 2H2O —-(I) • 2NO2- + O2———–> 2NO3- —-(II) • Ammonia is first oxidised to nitrite by the bacteria nitrosomonas and/or nitrococcus. The nitrite is further oxidised to nitrate with the help of the bacterium nitrobacter. These steps are called nitrification These nitrifying bacteria are chemoautotrophs. • The nitrate thus formed is absorbed by plants and is transported to the leaves. In leaves, it is reduced to form ammonia that finally forms the amine group of amino acids. Nitrate present in the soil is also reduced to nitrogen by the process of denitrification. Denitrification is carried by bacteria pseudomonas and thiobacillus.
  • 17. Nitrogen fixation • Certain prokaryotic species are capable of fixing nitrogen. Reduction of nitrogen to ammonia by living organisms is called biological nitrogen fixation. The enzyme, nitrogenase which is capable of nitrogen reduction is present exclusively in prokaryotes. Such microbes are called n2- fixers.
  • 18. • The nitrogen-fixing microbes could be free-living or symbiotic. • Examples of free-living nitrogen-fixing aerobic microbes are azotobacter and beijernickia • Rodospirillum is anaerobic • bacillus free-living, anaerobic • cyanobacteria such as anabaena and nostoc are also free-living nitrogen-fixers.
  • 19. Symbiotic nitrogen fixation • the legume-bacteria relationship. Species of rod-shaped Rhizobium has such relationship with the roots of several legumes such as alfalfa, sweet clover, sweet pea, lentils, garden pea, broad bean, clover beans • The most common association on roots is as nodules. These nodules are small outgrowths on the roots. • The microbe, frankia, also produces nitrogen-fixing nodules on the roots of nonleguminous plants (e.G., Alnus). • Both rhizobium and frankia are freeliving in soil, but as symbionts, can fix atmospheric nitrogen.
  • 20. Root nodules • Root nodules are pink in colour.This is due to the presence of leguminous haemoglobin or leg- haemoglobin.
  • 21. Nodule formation • Nodule formation involves a sequence of multiple interactions between rhizobium and roots of the host plant. • 1.Rhizobia multiply and colonise the surroundings of roots and get attached to epidermal and root hair cells. • 2.The root-hairs curl and the bacteria invade the root-hair. • 3,An infection thread is produced carrying the bacteria into the cortex of the root, where they initiate the nodule formation in the cortex of the root. • 4.Then the bacteria are released from the thread into the cells which leads to the differentiation of specialised nitrogen fixing cells. • The nodule thus formed, establishes a direct vascular connection with the host for exchange of nutrients
  • 27. Nitrogenase • The enzyme nitrogenase is highly sensitive to the molecular oxygen; it requires anaerobic conditions. • the nodule contains an oxygen scavenger called leg-haemoglobin
  • 28. • N2 +8E- +8H+ +16ATP—–>2NH3 +H2 +16ADP+16P1 • the ammonia synthesis by nitrogenease requires a very high input of energy (8 ATP for each NH3 produced). The energy required, thus, is obtained from the respiration of the host cells
  • 29. FATE OF AMMONIA: • At physiological ph, the ammonia is protonated to form nh4+ (ammonium) ion. While most of the plants can assimilate nitrate as well as ammonium ions, the latter is quite toxic to plants and hence cannot accumulate in them. Let us now see how the nh4+ is used to synthesise amino acids in plants. There are two main ways in which this can take place:
  • 30. (i) Reductive amination : • In these processes, ammonia reacts with α-ketoglutaric acid and forms glutamic acid
  • 31. (ii) Transamination • : It involves the transfer of amino group from one amino acid to the keto group of a keto acid. Glutamic acid is the main amino acid from which the transfer of NH2, the amino group takes place and other amino acids are formed through transamination. The enzyme transaminase catalyses all such reactions.
  • 32. Amides • The two most important amides – asparagine and glutamine – found in plants are a structural part of proteins. • They are formed from two amino acids, namely aspartic acid and glutamic acid, respectively, by addition of another amino group to each. The hydroxyl part of the acid is replaced by another nh2 – radicle. • Since amides contain more nitrogen than the amino acids, they are transported to other parts of the plant via xylem vessels. In addition, along with the transpiration stream • the nodules of some plants (e.G., Soyabean) export the fixed nitrogen as ureides. These compounds also have a particularly high nitrogen to carbon ratio.