3. Materials
• Plant Material- Egypt, Lake Marriott
• Preparation of Aqueous extract- 1.5 g plant in
30 ml distilled water, heated at 80 degree,
followed by centrifugation at 4000 rpm.
4. Methods
• Total phenolic content determination- Folin- Ciocalteu assay. 1mL
aliquot of extract+7.5 ml of 0.2 M NaOH +3.5 mL of FC reagent.
• Synthesis and stability of Metal Nanoparticles: 1.5 ml of extract, 15ml
of 10^-3 M HAuCl4. H2O, AgNO3+ HAuCl4. H2O, AgNO3 (1:1 molar
ratio). Solution was heated at 80 deg for 15 min--Au, 30 min—Ag, Au-
Ag.
• Studying the effect of different concentrations (10^-2, 10^-3, 10^-4) of
the precursor salt, reaction times (10, 20, 30 ,40, 50 min)
temperatures, and Ph Conditions (2,3.5,4,6.5,12), molar ratio (0:1,
1:3,1:1,3:1,1:0).
• Size, morphology, composition were investigated in the study.
10. Conclusion
• Total phenolic content= 7.4 micro gram gallic
acid equivilants/ml
• Alloy nature of bimetallic particles was
confirmed at pH-12
• Flavonoids and proteins are mainly
responsible for the stabilization of the
nanoparticles.
11. Reference
• AbdelHamid, A. A.; Al-Ghobashy, M. A., Fawzy, M.; Mohamed, M.B.; Abdel-
Mottaleb, Mohamed M.S.A Abdel-Mottaleb. Phytosynthesis of of Au, Ag, and
Au−Ag Bimetallic Nanoparticles Using Aqueous Extract of Sago
Pondweed(potamogeton pectinatus L.) ACS Sustainable Chem. Eng. 2013, 1, 1520-
1529.