Goodwill of Central Arizona and the City of Peoria are hosting a job and opportunity fair on November 8, 2012 from 10am to 3pm at the Peoria Sports Complex Stadium. Attendees will have the opportunity to meet with over 100 Arizona companies representing a wide range of industries. In addition to meeting with potential employers, attendees can learn about starting their own business from advisors in the Entrepreneurial Corner. The event is free and open to the public. Pre-registration is encouraged online for early access to employer exhibitors.
Dr Adam Clore discusses uses for gBlocks® Gene Fragments in the context of the 2015 iGEM competition. Dr Clore also describes how iGEM teams can register to receive 20 kb of free gBlocks Gene Fragments for their projects.
In this presentation, learn how IDT xGen® Lockdown® Probes help you capture target regions with a high degree of uniformity and specificity. You will also learn about combining probes to build your own panels or augment existing panels. This flexibility delivers the same high degree of uniform coverage with lower cost. Learn how the xGen Predesigned Gene Capture Pools will allow you to:
- Start with small reaction volumes to design and optimize new panels at lower cost, with less waste
- Build a cost-effective custom panel targeting specific UTR, introns, or intergenic regions of interest, as well as CDS
- Enhance existing panels with expanded target space or improved capture
- Design your own custom plate with individual gene targets in each well
In addition to a standard gene synthesis service, IDT offers a novel, rapid, and reliable method to build and clone the genes you need at a fraction of the cost of full gene synthesis services. gBlocks® Gene Fragments are double-stranded, sequence-verified DNA blocks of length 125–750 bp. Their high sequence fidelity and rapid delivery time make gBlocks Gene Fragments ideal for a large range of synthetic biology applications. In this presentation, Dr Adam Clore reviews a variety of uses of gBlocks fragments, including CRISPR-based genome modification, qPCR and HRM controls, and the assembly of gene fragments using the Gibson Assembly® Method.
Functional analysis by mRNA knockdown using siRNAs is now routine in many molecular biology labs. However, many RNAi experiments fail due to diversion from simple, good practices. This webinar will review the steps leading to successful siRNA experiments, including:
- Understanding the target transcript
- siRNA selection
- Choosing the cell type
- Validating the assay
- Including appropriate biological controls
The National Center for Biotechnology Information (NCBI) provides one of the most extensive sets of web-based tools for biological research. The tools are indispensable when planning genomics experiments, including for qPCR, NGS, and CRISPR. In this presentation, Dr Matt McNeill takes a practical look at getting started with the wealth of NCBI tools, and shares some relevant tips to help you sift through the tools and options that we regularly use. In particular, he focuses on commonly adjusted parameters that will allow you to more effectively use the powerful Basic Local Alignment Algorithm Tool (BLAST) to identify off-target hybridization/annealing events. Dr McNeill also covers practical examples using NCBI tools to design assays.
Dr Adam Clore discusses uses for gBlocks® Gene Fragments in the context of the 2015 iGEM competition. Dr Clore also describes how iGEM teams can register to receive 20 kb of free gBlocks Gene Fragments for their projects.
In this presentation, learn how IDT xGen® Lockdown® Probes help you capture target regions with a high degree of uniformity and specificity. You will also learn about combining probes to build your own panels or augment existing panels. This flexibility delivers the same high degree of uniform coverage with lower cost. Learn how the xGen Predesigned Gene Capture Pools will allow you to:
- Start with small reaction volumes to design and optimize new panels at lower cost, with less waste
- Build a cost-effective custom panel targeting specific UTR, introns, or intergenic regions of interest, as well as CDS
- Enhance existing panels with expanded target space or improved capture
- Design your own custom plate with individual gene targets in each well
In addition to a standard gene synthesis service, IDT offers a novel, rapid, and reliable method to build and clone the genes you need at a fraction of the cost of full gene synthesis services. gBlocks® Gene Fragments are double-stranded, sequence-verified DNA blocks of length 125–750 bp. Their high sequence fidelity and rapid delivery time make gBlocks Gene Fragments ideal for a large range of synthetic biology applications. In this presentation, Dr Adam Clore reviews a variety of uses of gBlocks fragments, including CRISPR-based genome modification, qPCR and HRM controls, and the assembly of gene fragments using the Gibson Assembly® Method.
Functional analysis by mRNA knockdown using siRNAs is now routine in many molecular biology labs. However, many RNAi experiments fail due to diversion from simple, good practices. This webinar will review the steps leading to successful siRNA experiments, including:
- Understanding the target transcript
- siRNA selection
- Choosing the cell type
- Validating the assay
- Including appropriate biological controls
The National Center for Biotechnology Information (NCBI) provides one of the most extensive sets of web-based tools for biological research. The tools are indispensable when planning genomics experiments, including for qPCR, NGS, and CRISPR. In this presentation, Dr Matt McNeill takes a practical look at getting started with the wealth of NCBI tools, and shares some relevant tips to help you sift through the tools and options that we regularly use. In particular, he focuses on commonly adjusted parameters that will allow you to more effectively use the powerful Basic Local Alignment Algorithm Tool (BLAST) to identify off-target hybridization/annealing events. Dr McNeill also covers practical examples using NCBI tools to design assays.
Struggling with low editing efficiency or delivery problems? IDT has developed a simple and affordable CRISPR-Cas9 solution that outperforms other methods. In this presentation we present the advantages of using a Cas9:tracrRNA:crRNA ribonucleoprotein (RNP) complex in genome editing experiments, and explain why it is the most efficient driver for genome editing compared to alternative methods, such as expression plasmids or the use of sgRNAs. We also review RNP delivery using cationic lipids and electroporation, and provide tips for optimized transfection in your system.
Carlson Wireless Technologies, the global leader in TV White Space technology, has developed the FCC certified RuralConnect®, which uses UHF TV channels to provide broadband data to remote areas. This spectrum provides deep coverage for long-distant, non-line-of-sight (NLOS), internet connectivity to under-served communities over hills and through foliage. Carlson also offers a range of radio products for high-capacity data and voice delivery, including the TrailBlazer™ and LongHaul™.
IDT provides a range of solutions for targeted next generation sequencing. Labs processing hundreds to thousands of samples can create highly uniform, custom panels using xGen® Lockdown Probes. The new xGen Acute Myeloid Leukemia (AML) panel is a predesigned set of Lockdown Probes that captures 260 genes identified by whole genome and exome sequencing of 200 patient samples. The AML panel can be used as stand-alone or customized with additional probes to detect other targets of interest.
qPCR assays using intercalating dyes, such as SYBR® Green dye, are an economical and effective tool for measuring gene expression. To interpret intercalating dye assays, users need to know how to analyze melt curves, and understand the benefits and limitations of melt curve analysis. In this presentation, Nick Downey, PhD, covers melt curve basics and shares examples of multiple peaks due to suboptimal sample prep, primer dimers, and asymmetric GC content of amplicons. He demonstrates troubleshooting strategies. Experienced and novice users will benefit from an overview of uMeltSM software, developed by the Wittwer lab at the University of Utah, that can predict the melt profile of your assay before you run your experiment.
Improved Reagents & Methods for Target Enrichment in Next Generation Sequencing, presented by Dr Mark Behlke, Chief Scientific Officer at Integrated DNA Technologies
The CRISPR/Cas9 system has emerged as one of the leading tools for modifying genomes of organisms ranging from E. coli to humans. Additionally, the simple gene targeting mechanism of CRISPR technology has been modified and adapted to other applications that include gene regulation, detection of intercellular trafficking, and pathogen detection. With a wealth of methods for introducing Cas9 and gRNAs into cells, it can be challenging to decide where to start. In this presentation, Dr Adam Clore describes the CRISPR mechanism and some of the most prominent uses for CRISPR, along with methods where IDT technologies can assist scientists in designing, testing, and executing a variety of CRISPR-mediated experiments. For more informaton, visit: http://www.idtdna.com/crispr
Target enrichment enables researchers to focus their next generation sequencing (NGS) efforts on regions of interest, allowing them to obtain more sequencing data relevant to their study. In-solution target capture is a method of enrichment using oligonucleotide probes directed to specific regions within a genome. Target capture can be used to enrich multiple samples simultaneously, reducing the cost per sample, while using individually synthesized probes allows researchers to construct gene panels that can be optimized over time.
Struggling with low editing efficiency or delivery problems? IDT has developed a simple and affordable CRISPR-Cas9 solution that outperforms other methods. In this presentation we present the advantages of using a Cas9:tracrRNA:crRNA ribonucleoprotein (RNP) complex in genome editing experiments, and explain why it is the most efficient driver for genome editing compared to alternative methods, such as expression plasmids or the use of sgRNAs. We also review RNP delivery using cationic lipids and electroporation, and provide tips for optimized transfection in your system.
Carlson Wireless Technologies, the global leader in TV White Space technology, has developed the FCC certified RuralConnect®, which uses UHF TV channels to provide broadband data to remote areas. This spectrum provides deep coverage for long-distant, non-line-of-sight (NLOS), internet connectivity to under-served communities over hills and through foliage. Carlson also offers a range of radio products for high-capacity data and voice delivery, including the TrailBlazer™ and LongHaul™.
IDT provides a range of solutions for targeted next generation sequencing. Labs processing hundreds to thousands of samples can create highly uniform, custom panels using xGen® Lockdown Probes. The new xGen Acute Myeloid Leukemia (AML) panel is a predesigned set of Lockdown Probes that captures 260 genes identified by whole genome and exome sequencing of 200 patient samples. The AML panel can be used as stand-alone or customized with additional probes to detect other targets of interest.
qPCR assays using intercalating dyes, such as SYBR® Green dye, are an economical and effective tool for measuring gene expression. To interpret intercalating dye assays, users need to know how to analyze melt curves, and understand the benefits and limitations of melt curve analysis. In this presentation, Nick Downey, PhD, covers melt curve basics and shares examples of multiple peaks due to suboptimal sample prep, primer dimers, and asymmetric GC content of amplicons. He demonstrates troubleshooting strategies. Experienced and novice users will benefit from an overview of uMeltSM software, developed by the Wittwer lab at the University of Utah, that can predict the melt profile of your assay before you run your experiment.
Improved Reagents & Methods for Target Enrichment in Next Generation Sequencing, presented by Dr Mark Behlke, Chief Scientific Officer at Integrated DNA Technologies
The CRISPR/Cas9 system has emerged as one of the leading tools for modifying genomes of organisms ranging from E. coli to humans. Additionally, the simple gene targeting mechanism of CRISPR technology has been modified and adapted to other applications that include gene regulation, detection of intercellular trafficking, and pathogen detection. With a wealth of methods for introducing Cas9 and gRNAs into cells, it can be challenging to decide where to start. In this presentation, Dr Adam Clore describes the CRISPR mechanism and some of the most prominent uses for CRISPR, along with methods where IDT technologies can assist scientists in designing, testing, and executing a variety of CRISPR-mediated experiments. For more informaton, visit: http://www.idtdna.com/crispr
Target enrichment enables researchers to focus their next generation sequencing (NGS) efforts on regions of interest, allowing them to obtain more sequencing data relevant to their study. In-solution target capture is a method of enrichment using oligonucleotide probes directed to specific regions within a genome. Target capture can be used to enrich multiple samples simultaneously, reducing the cost per sample, while using individually synthesized probes allows researchers to construct gene panels that can be optimized over time.
1. Goodwill of Central Arizona and the City of Peoria Present
Make Your Career Happen!
Job and Opportunity Fair
November 8, 2012
10 a.m. to 3 p.m.
Peoria Sports Complex Stadium
16101 N. 83rd Avenue
Peoria, Arizona 85382
Attendees will have an opportunity to meet Arizona
Companies representing a wide range of industries.
AASK Dish Network Nightingale Home Care
ADDECO DOC Nordstrom
Adelante Duncan and Son Lines, OUR 365 Newborn
Inc. Photography
AFR Home Loans
EDMC Online Higher Pampered Chefs
Alliance of Arizona Education
Nonprofits Peoria Chamber
Edward Jones
ALW Sourcing LLC Investments Peoria Good Shepherd
American Valet Eldorado | a division of Peoria Police Dept
Anthem College MphasiS Peoria Unified School
Empereon Marketing, District #11
APAC Customer Service
Inc. LLC PEPSICO
Appleone (Peoria) Esurance PetSmart
Arizona Commerce Family Support Services Phoenix Packaging
Authority Farm Bureau Financial Operations
Arizona Department of Services Phoenix SCORE
Health Services Farmers Insurance Pride Group
Army National Guard Fastenal Company Primerica
Art Institute First American Home Progressive Insurance
Auction Systems Buyers Protection
PSA- Behavioral Health
AZ DEQ Five Star Concrete Agency
Services, Inc.
Bank of America Quality Care Network
Fry’s Food Stores
BBVA Compass Bank RB Balch IT Consulting
Glendale Elementary
Benevilla School District #40 REC Solar
Best Bill Global Technology Republic Media /
Resources, LLC Careerbuilder
BioScreen Clinical
Services Goodwill Revana
CalPortland Green Tree Servicing Right At Home
Camino del Sol Funeral Griffon Printing Shop Riveria Broadcasting
center
Holland America S.E.E.K. Arizona
Centerplate Princess Sapphire Technology
Cintas IES Commercial Group
Circle K International Cruise & Scentsy
City of Peoria, AZ Excursions, Inc. Schwab
City of Phoenix Intrepid Tool Industries, Snyder’s-Lance
LLC
City of Phoenix Finance Solavei
Department IPRO Tech, Inc.
Southwest Network
Collins College Job Examiner
State Farm
Comfort Keepers K Force
Sunstate Equipment
Comprehensive Risk Kelly Services
Services (CRS) Syncis
LegalShield
Computer Skills Institute Localwork.com TCF Bank
Convergence Marketing Lowe’s TECA ROOFING
Convergence Marketing Luke Air Force Base Training to YOU
CORESTAFF Trine University
Macayo’s Mexican
Corinthian Colleges, Inc Restaurant U.S. Small Business
Administration
COUNTRYWIDE Manpower
®Financial U-Haul
Maricopa County
Cox Communications US NAVY
Maricopa Skill Center
Credit Union West Verengo Solar
Maricopa Workforce
CyraCom International, Connections Walmart- Avondale
Inc. Mingus Mountain Washington Elementary
DES Academy
Wells Fargo
Dignity Health NCO Financial
Western Career Institute
Discover Neutron Industries
Western International
University
Registration:
We encourage all Attendees to Register on-line at
www.peoriaaz.gov/jobfair to receive early access to
the Employer Exhibitors
We will also have an Entrepreneurial Corner so you
may learn from advisors such as the Small Business
Administration how to start your own business.
Don’t miss out on this FREE job and opportunity fair
to jump start your career.
Sponsored by
Economic Development Services Department
9875 N 85th Ave • Peoria, AZ 85345 • www.peoriaed.com