Patisiran is a siRNA therapeutic used to treat hereditary transthyreti.pdf
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Patisiran is a siRNA therapeutic used to treat hereditary transthyretin-mediated amyloidosis. It works by using the RNAi pathway which destroys mRNA in the cytoplasm preventing translation of the disease-causing proteins. For a research project you want to observe the knock down of transthyretin mRNA in different cell populations in a model organisms. Describe a Hairpin Chain reaction based method to profile the location and presence of transthyretin mRNA. You do not need to discuss the synthesis of any component but describe some possible sequences and use diagrams to show how you would accomplish this..
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c. P6,000
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