1) The document discusses Nikon's suggested imaging configuration for biological research, including microscopes, cameras, and software. 2) Nikon's approach involves providing total system solutions centered around their microscopes and including peripheral products from Nikon and third parties to meet various applications like 3D imaging, timelapse, and multipoint imaging. 3) The suggested configuration can provide multi-observation from 3D to 6D including variations in X,Y,Z,T, wavelength, and multipoint to suit needs like live cell imaging and fluorescence/DIC timelapse with Nikon's Perfect Focus System.

1. Nikon Suggested
Imaging Configuration
NIKON “NIS-ELEMENTS” TRAINING NEW PRODUCTS MEETING 2006
February 7,2006
Singapore
Nikon Instech Co.,Ltd.
Sachiko Morimoto

2. Purpose of
Current Biological Research Market
living cell observation → imaging
→ presentation & analysis
• Microscope :motorization, high quality image etc.
• CCD Camera: high resolution, high speed etc.
• Software: easy to use, multiple functions, continues
improvement (up-grade)

3. 3D imaging
Timelapse
(Time series
observation)
Microscope
Main body
Eyepieces
Nosepiece
Stage
Objective lens
Condenser
DSC
NISElements
Nikon’s approach for System Solution
Periphery
products
(Nikon or
3rd
party)
Application
1. Microscope hardware(~2003)
2. Start Imaging equipments & software(2004~2005)
3.Emphasize on System products for each application (2006~)
and more ・・・

4. Overview of Nikon System Solution
• Microscope: TE2000 series, 90i
• Camera:DS5M,DS5Mc,DS2MBWc,DS2M,DS2Mc,
DXM1200F, DXM1200c
• Software :NIS Elements AR/BR + plug in
Nikon System Solution
Some third party’s
accessories if necessary.
(ex. Motorized shutter,
motorized stage, incubator
system etc.)

5. Merit of System offer sales
• If we can offer whole system to your
customers, it can give more reliability to
customers.
• Customer can receive full support from
Nikon.
• It is easy for customers to choose a
system from one manufacture.

6. Nikon’s suggested configuration
~ Multi observation from 3D to 6D X,Y,Z,T,λ,Multipoint ~
X
YZ
Multi point
TimeTime
For living cell and
observing the changes in
time series.
For multiple stained sample
For thick sample
and 3D imaging
Especially for well plate users

7. Application
• Fluorescence
• DIC
• Multi Fluorescence
• Multi Fluorescence ＋ DIC
• Fluorescence Timelapse （ with PFS)
• DIC Timelapse （ with PFS)
• Fluorescence + DIC Timelapse （ with PFS)
• Multi Fluorescence + DICTimelapse （ with PFS)
• Multipoint
• EDF
• Fluorescence Deconvolution
• Real time Deconvolution

8. Third party accessories
Sutter : EX/BA filter wheel
Lambda 10
Prior: Motorized stage Pro
Scan H117E2
UNIBLITZ: Shutter for
Epi/Dia

9. Third party accessories
CCD Camera : Roper Scientific

10. Nikon’s Current System
Motorized stage
PFS （ AF ） Motorized
shutter
CO2 chamber
controller
Light source
EX filter
wheel
CCD Camera
DIA Motorized shutter
CO2chamber(TOKAIHIT)
BA filter
wheel
PC （ NIS-
element
Objective lens

11. Nikon Exclusive Objective lens
 Temperature correction *World First!*World First!
Aberration is corrected in temperature 23-37degrees
･･･ Friendly for living cell
CFI Plan Apo TIRF60xoil N.A.1.49, W.D.0.13
CFI Plan Apo TIRF100xoil N.A.1.49, W.D. 0.12
Nikon Exclusive Perfect Focus System
 Optical offset function maintain the focus position of
your interested plane by ‘m sec’‘m sec’ order.
 Stable focusing for time-lapse imaging
 For 2 to 3 days or longer time lapse imaging.
 Using IR LED for the light source
TE2000E with New TIRF obj. and NIS Elements are the bestTE2000E with New TIRF obj. and NIS Elements are the best
choice for living cell time lapse system!choice for living cell time lapse system!

12. Market Demands
More & More
digital imaging
High Quality
Imaging
PC control for
various applications
Let’s work together as
Total Solution Provider!

13. Thank you !