basic of plant tissue culture

1. p
Plant Tissue Culture

2. ❏ Tissue culture involves the use of small pieces of plant tissue (explants)
which are cultured in a nutrient medium under sterile conditions. (ex vivo)
❏ These explants are transferred on to the nutrient media prepared from agar
and whole plants can be regenerated through in vitro culture.This technique
is also known as micropropagation.
But why PLANT TISSUE CULTURE
❏ to fulfil the required demand of crops
❏ for the production of disease-free plants and increase plant yield
❏ It makes use of parts of a plant to generate multiple copies of the plant in a very short
duration.
❏ used for commercial production of plants
as well as for plant research.

4. Types of Plant tissue culture
1. Seed Culture
2. Embryo Culture
3. Callus Culture
4. Organ Culture
5. Protoplast Culture
6. Anther Culture
7. Pollen Culture
8. Single Cell Culture
9. Suspension Culture
10. Somatic Embryogenesis

5. Steps of Tissue Culture

7. Some of the basic requirements for plant tissue culture are:
(i) Washing area—the place to wash glassware, plasticware and other labware
used in PTC. Washed labware need to be stored at a clean and dry place.

8. (ii) Various media components for the preparation of Nutrient Media.
(iii) Facility to sterilise nutrient media and store media at low temperatures

9. (iv) Facilities for maintenance of cultured tissues in aseptic conditions under
controlled environment i.e., light, temperature and humidity

10. Applications of Plant Tissue Culture