The document describes using the R statistical software to analyze flow cytometry data. It outlines the standard workflow which includes reading data files, performing transformations and compensation, gating the sperm population, normalizing the data, identifying subpopulations, and obtaining statistics. A practical example analyzes a semen sample stained for apoptosis, membrane integrity, and mitochondria that was evaluated at two time points. The results identify changes in the subpopulation percentages over time.