The document outlines steps for culturing and inducing E.coli to produce TNFα, GM-CSF, and MOC proteins between November 10-11. On November 14, the proteins will be isolated from the culture suspension according to a specific purification protocol. On November 17, SDS-PAGE will be used to check production of the proteins in crude and supernatant samples. Depending on whether the proteins are in the pellet or supernatant fractions, the purification protocol will be continued under denaturing or native conditions on November 21 or 24. The purification protocol will be finished on November 28. If needed, the sample will be dialyzed to remove urea starting December 1 and the amount of biologically active TNFα will be measured using a