The study examined the effects of the biocide triclosan on the growth of five species of marine algae: Isochrysis galbana, Tetraselmis chuii, Nannochloropsis oculata, Rhodomonas salina, and Chaetocerus gracilis. Algae were exposed to various concentrations of triclosan over time periods of 24, 48, 72, and 96 hours. Results showed that higher triclosan concentrations and longer exposure times inhibited algae growth rates in a dose-dependent manner. Estimated EC50 values were also determined for each algae species based on triclosan concentration curves.
Loadings of Pharmaceutical & Person Care Products & Endocrine Disrupting Chem...v2zq
Loadings of Pharmaceutical & Person Care Products & Endocrine Disrupting Chemicals from Small Rural Streams into an Urban Drinking Water Source - Resources for Healthy Children www.scribd.com/doc/254613619 - For more information, Please see Organic Edible Schoolyards & Gardening with Children www.scribd.com/doc/254613963 - Gardening with Volcanic Rock Dust www.scribd.com/doc/254613846 - Double Food Production from your School Garden with Organic Tech www.scribd.com/doc/254613765 - Free School Gardening Art Posters www.scribd.com/doc/254613694 - Increase Food Production with Companion Planting in your School Garden www.scribd.com/doc/254609890 - Healthy Foods Dramatically Improves Student Academic Success www.scribd.com/doc/254613619 - City Chickens for your Organic School Garden www.scribd.com/doc/254613553 - Huerto Ecológico, Tecnologías Sostenibles, Agricultura Organica www.scribd.com/doc/254613494 - Simple Square Foot Gardening for Schools - Teacher Guide www.scribd.com/doc/254613410 - Free Organic Gardening Publications www.scribd.com/doc/254609890 ~ gmu.edu
This document describes a study that used a factorial design of experiments approach to parameterize and optimize the synthesis of PLGA nanoparticles. Three factors - stirring speed, aqueous phase volume, and PLGA concentration - were varied to determine their effects on particle size and polydispersity index (PDI). Statistical analysis found that the aqueous phase volume and PLGA concentration significantly affected PDI. Validation experiments showed results close to the response surface model's predictions. The design of experiments approach achieved the goals in fewer experiments than the typical one-factor-at-a-time method. Future work will involve screening additional factors using a fractional factorial design.
ABSTRACT- Plants of Polygalaceae family are source of several compounds such as xanthones, coumarins, phenols,
triterpenes, steroids, pyrones derivatives and alkaloids. These plants contain chemical compounds with a large spectrum of
biological activities, including anti-depressant and anti-angiogenic. Moutabea guianensis is an Amazonian species
belongs to the Polygalaceae family. In this work, from roots of M. guianensis were isolated a new xanthone,
3,8-dihydroxy-1,2,4,5-tetramethoxyxanthone, named moutabeone D, and one known xanthone, 1,3,5-trihydroxy-2-
methoxyxanthone. Column chromatography on silica gel and semi-preparative HPLC led the isolation of these
compounds. The structures were elucidated by spectroscopic data (HRESIMS, UV, IR, 1D and 2D NMR).
Key-words- Moutabea guianensis, Polygalaceae, Xanthones
This article examines the larvicidal activity of extracts from Excoecaria agallocha roots against Culex quinquefasciatus mosquito larvae. Crude hexane extracts had an LC50 of 315 ppm. Fractionation yielded two active fractions with LC50 values of 61.2 ppm and 74.5 ppm. Fraction 3 contained the compound n-triacontane, identified as a linear alkane through NMR and mass spectrometry. Previous studies have found that linear and branched alkanes from other plants can have larvicidal and antifeedant effects against mosquitoes.
The document summarizes a dissertation proposal that aims to assess mercury distribution and potential health risks in the Grand Bay National Estuarine Research Reserve. The study will measure mercury levels in fish, water, and sediment samples collected from the reserve using cold vapor atomic absorption spectrometry. The results will help inform policies for managing mercury contamination and assess spatial and temporal trends and risks from mercury exposure.
The study examined the effects of the biocide triclosan on the growth of five species of marine algae: Isochrysis galbana, Tetraselmis chuii, Nannochloropsis oculata, Rhodomonas salina, and Chaetocerus gracilis. Algae were exposed to various concentrations of triclosan over time periods of 24, 48, 72, and 96 hours. Results showed that higher triclosan concentrations and longer exposure times inhibited algae growth rates in a dose-dependent manner. Estimated EC50 values were also determined for each algae species based on triclosan concentration curves.
Ecotoxicity & Risk Assessment of Mercury in the Grand Bay National Estuarine Research Reserve:
Profiling Mercury Distribution in the NERR by Cold Vapor Atomic Absorption Spectrometery
Reuters: Pictures of the Year 2016 (Part 2)maditabalnco
This document contains 20 photos from news events around the world between January and November 2016. The photos show international events like the US presidential election, the conflict in Ukraine, the migrant crisis in Europe, the Rio Olympics, and more. They also depict human interest stories and natural phenomena from various countries.
Loadings of Pharmaceutical & Person Care Products & Endocrine Disrupting Chem...v2zq
Loadings of Pharmaceutical & Person Care Products & Endocrine Disrupting Chemicals from Small Rural Streams into an Urban Drinking Water Source - Resources for Healthy Children www.scribd.com/doc/254613619 - For more information, Please see Organic Edible Schoolyards & Gardening with Children www.scribd.com/doc/254613963 - Gardening with Volcanic Rock Dust www.scribd.com/doc/254613846 - Double Food Production from your School Garden with Organic Tech www.scribd.com/doc/254613765 - Free School Gardening Art Posters www.scribd.com/doc/254613694 - Increase Food Production with Companion Planting in your School Garden www.scribd.com/doc/254609890 - Healthy Foods Dramatically Improves Student Academic Success www.scribd.com/doc/254613619 - City Chickens for your Organic School Garden www.scribd.com/doc/254613553 - Huerto Ecológico, Tecnologías Sostenibles, Agricultura Organica www.scribd.com/doc/254613494 - Simple Square Foot Gardening for Schools - Teacher Guide www.scribd.com/doc/254613410 - Free Organic Gardening Publications www.scribd.com/doc/254609890 ~ gmu.edu
This document describes a study that used a factorial design of experiments approach to parameterize and optimize the synthesis of PLGA nanoparticles. Three factors - stirring speed, aqueous phase volume, and PLGA concentration - were varied to determine their effects on particle size and polydispersity index (PDI). Statistical analysis found that the aqueous phase volume and PLGA concentration significantly affected PDI. Validation experiments showed results close to the response surface model's predictions. The design of experiments approach achieved the goals in fewer experiments than the typical one-factor-at-a-time method. Future work will involve screening additional factors using a fractional factorial design.
ABSTRACT- Plants of Polygalaceae family are source of several compounds such as xanthones, coumarins, phenols,
triterpenes, steroids, pyrones derivatives and alkaloids. These plants contain chemical compounds with a large spectrum of
biological activities, including anti-depressant and anti-angiogenic. Moutabea guianensis is an Amazonian species
belongs to the Polygalaceae family. In this work, from roots of M. guianensis were isolated a new xanthone,
3,8-dihydroxy-1,2,4,5-tetramethoxyxanthone, named moutabeone D, and one known xanthone, 1,3,5-trihydroxy-2-
methoxyxanthone. Column chromatography on silica gel and semi-preparative HPLC led the isolation of these
compounds. The structures were elucidated by spectroscopic data (HRESIMS, UV, IR, 1D and 2D NMR).
Key-words- Moutabea guianensis, Polygalaceae, Xanthones
This article examines the larvicidal activity of extracts from Excoecaria agallocha roots against Culex quinquefasciatus mosquito larvae. Crude hexane extracts had an LC50 of 315 ppm. Fractionation yielded two active fractions with LC50 values of 61.2 ppm and 74.5 ppm. Fraction 3 contained the compound n-triacontane, identified as a linear alkane through NMR and mass spectrometry. Previous studies have found that linear and branched alkanes from other plants can have larvicidal and antifeedant effects against mosquitoes.
The document summarizes a dissertation proposal that aims to assess mercury distribution and potential health risks in the Grand Bay National Estuarine Research Reserve. The study will measure mercury levels in fish, water, and sediment samples collected from the reserve using cold vapor atomic absorption spectrometry. The results will help inform policies for managing mercury contamination and assess spatial and temporal trends and risks from mercury exposure.
The study examined the effects of the biocide triclosan on the growth of five species of marine algae: Isochrysis galbana, Tetraselmis chuii, Nannochloropsis oculata, Rhodomonas salina, and Chaetocerus gracilis. Algae were exposed to various concentrations of triclosan over time periods of 24, 48, 72, and 96 hours. Results showed that higher triclosan concentrations and longer exposure times inhibited algae growth rates in a dose-dependent manner. Estimated EC50 values were also determined for each algae species based on triclosan concentration curves.
Ecotoxicity & Risk Assessment of Mercury in the Grand Bay National Estuarine Research Reserve:
Profiling Mercury Distribution in the NERR by Cold Vapor Atomic Absorption Spectrometery
Reuters: Pictures of the Year 2016 (Part 2)maditabalnco
This document contains 20 photos from news events around the world between January and November 2016. The photos show international events like the US presidential election, the conflict in Ukraine, the migrant crisis in Europe, the Rio Olympics, and more. They also depict human interest stories and natural phenomena from various countries.
Expt. 7 Bioassay of acetylcholine using rat ileum by four point bioassayVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 2 Bioassay of acetylcholine using rat ileum by four point bioassayVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Response of aquatic fern(Azolla), to watercontaminationKavitha Cingam
The document summarizes a study on the response of the aquatic fern Azolla to contamination by the antibiotic ciprofloxacin (Cipro). The study investigated Azolla's ability to uptake Cipro and the effects on its nitrogen-fixing process. The results showed that Azolla is able to uptake concentrations of Cipro above levels that classify plants as hyperaccumulators. Exposure to Cipro negatively impacted the heterocyst/vegetative cell ratio, nitrogenase activity, total nitrogen, and disrupted photosynthesis. This suggests that Cipro is toxic not only to the nitrogen-fixing cyanobacteria in Azolla but also to Azolla itself, demonstrating its potential use in phytoremediation of Cipro contamination
Application of Plasma in Food and AgricultureSITHUHan3
This document summarizes research on using cold plasma in agriculture. It discusses how cold plasma can be used to:
1) Decontaminate seeds by reducing fungal and bacterial counts on seed surfaces through plasma exposure, achieving log reductions in microbes (Section 8.2).
2) Enhance seed germination by accelerating germination rates and increasing sprout lengths through plasma-induced changes to seed coat permeability and plant hormone levels (Section 8.3).
3) Positively impact plant growth by producing reactive oxygen and nitrogen species through plasma that increase nutrient uptake and growth factors when plants are irrigated with plasma-treated water (Section 8.4).
This document summarizes a study that investigated the cytotoxic activity of a chloroform extract and four diterpenes isolated from Salvia ballotiflora against five cancer cell lines. The extract and isolated compounds were tested using an MTT assay to determine their IC50 values. 19-Deoxyisoicetexone had the greatest effect on HeLa cells with an IC50 of 3.2 μg/ml, while the chloroform extract showed the best cytotoxicity against A549 cells with an IC50 of 2.29 μg/ml. These effects were similar to the IC50 values of cisplatin in the respective cell lines. The study isolated and identified the active compounds from S. ballotiflora
IJRET : International Journal of Research in Engineering and Technology is an international peer reviewed, online journal published by eSAT Publishing House for the enhancement of research in various disciplines of Engineering and Technology. The aim and scope of the journal is to provide an academic medium and an important reference for the advancement and dissemination of research results that support high-level learning, teaching and research in the fields of Engineering and Technology. We bring together Scientists, Academician, Field Engineers, Scholars and Students of related fields of Engineering and Technology
This document discusses a study on the effects of herbivory by brant geese and algal blooms on a Zostera marina seagrass community. The study used a factorial experiment to simulate different combinations of clipping/herbivory, algal additions, and nutrient additions. Results showed that clipping significantly reduced seagrass biomass and cover but did not affect its nutrient content. Clipping enhanced seagrass growth after treatment. Algal additions did not affect seagrass directly but a negative relationship was found between green macroalgae and seagrass biomass. Epiphyte biomass on seagrass was also reduced by clipping. Environmental factors like light and
Effect of Temperature on Stream Fish Energetics and Tolerance to Increasing U...Marylou Moore
ABSTRACT
Urban development threatens stream systems throughout the world and has been shown to correspond with predictable changes in fish assemblage attributes. These shifts in fish communities suggests that there may be species specific differences in tolerance to altered environmental conditions related to urbanization. Over the last three decades, a great amount of literature has focused on the development of community-level metrics for assessing biotic integrity of fishes within flowing waters. While these efforts have spawned a great deal of information on community and guild-level responses by stream fishes, it is unknown how individual species’ evolutionary adaptation and physiological acclimation correspond to their ability to tolerate drastic environmental change. This study investigated temperature-dependent responses of metabolism and thermal sensitivity in redbreast sunfish (Lepomis auritus) collected from sites representative of the lowest and highest degree of urban land use throughout the Etowah Watershed, Georgia. Standard metabolic rate (SMR) was shown to be significantly lower in fish drawn from the urban stream at both experimental temperatures (20 °C and 25 °C). Additionally, the urban population had a significantly higher metabolic scope at 25 °C than the non-urban population. These findings support the hypothesis that intraspecific differences in thermal tolerance may exist at localized scales within a small watershed. This study also provides the first known reports of the metabolic rate and aerobic scope of L. auritus.
This document discusses the effects of foliar application of liquid organic fertilizers on crop growth and quality. It begins with introducing liquid organic fertilizers and their benefits over solid organic fertilizers. The objectives are to understand the effects on crop growth, yield, and quality. Several tables show results of research experiments applying different liquid organic fertilizers like vermicompost wash, cow dung wash, and panchagavya on various crops like chickpeas, soybeans, and groundnuts. The results demonstrate increased plant growth parameters, yields, and quality from foliar application of liquid organic fertilizers compared to control treatments.
The extract of Vismia mexicana leaves was found to inhibit HIV-1. Two anthraquinones, vismiaquinone D and vismiaquinone, were isolated from the extract. The structures of the two compounds were determined by NMR and X-ray crystallography. Neither compound showed inhibition of HIV-1 reverse transcriptase, suggesting other compounds in the extract are responsible for the antiviral activity.
This document summarizes a new method for detecting human hormones in drinking water supplies using solid-phase extraction and liquid chromatography-mass spectrometry. The method monitors 9 hormones including estrogens and androgens. Hormones are extracted from water samples using SPE cartridges, separated via HPLC, and identified/quantified on a triple quadrupole mass spectrometer. Recoveries for the hormones from drinking water ranged from 90-124%, and the method provides well-differentiated mass spectrometry peaks for each hormone. The new method complies with EPA Method 539 for monitoring hormones in drinking water supplies.
This document summarizes an experiment evaluating the tolerance of potato genotypes to osmotic stress conditions in vitro. Forty-two potato genotypes from international centers and local varieties were screened under normal conditions and two levels of osmotic stress induced by sorbitol. Various drought tolerance traits such as shoot and root growth, biomass, and proline content were measured. Most traits were significantly affected by genotype, sorbitol treatment, and their interaction. Several genotypes performed relatively better under stress and were identified as potentially drought tolerant for further evaluation under field conditions.
This document summarizes a research study that developed a colorimetric assay using gold nanoparticles to detect beta-agonists. The assay can quickly detect beta-agonists and their analogs in serum, urine, and other liquid samples. The researchers determined the optimal conditions for detection and pretreated different liquid samples. They tested 13 types of beta-agonists in serum, urine, and milk samples and achieved detection limits down to 0.01 ng/mL. The colorimetric assay provides a fast, broad-spectrum method for screening beta-agonists in various samples with sensitivity comparable or better than other methods.
Dielectric parameters of ayurvedic medicines biological approacheSAT Journals
Abstract
The study of dielectric properties of biological materials is of great assistance in exploring the molecular structure and dynamics
of condensed matter. Time domain reflectometry in reflection mode has been used as a technique. A nonlinear least squares fit
method have been used to obtain dielectric parameters viz., static dielectric constant (ɛ0 ) and dielectric relaxation time (τ) at
temperatures 100C, 200C, 300C and 400C of Ayurvedic Medicines.
Keywords: Medicinal plants, Asava, Ethanol,Time domain reflectometry, Fourier transformation, Dielectric
constant, Relaxation time.
This document contains abstracts from two veterinary emergency and critical care conferences that took place in 2014: the International Veterinary Emergency and Critical Care Symposium (IVECCS) in Indianapolis, USA and the European Veterinary Emergency and Critical Care Society (EVECCS) Annual Congress in Prague, Czech Republic. The abstracts cover a range of topics including studies evaluating new diagnostic tests and treatments for emergency and critical care patients. They are organized by conference and then alphabetically by presenter within each small and large animal section.
STUDIES ON THE CHRONIC TOXICITY OF CHLOROQUINE 1948thierry jolaine
This document summarizes a study on the chronic toxicity of chloroquine, an antimalarial drug. In the study:
1) Two groups of 20 subjects each were given different dosage schedules of chloroquine for one year to study long-term toxicity - one group received 0.3 grams daily for 77 days then 0.5 grams weekly, the other received 0.5 grams weekly the whole time.
2) Toxic effects observed included visual difficulties, hair bleaching in blondes, mild skin eruptions in two subjects, slight weight loss, and temporary electrocardiogram changes in the daily dosage group.
3) Plasma chloroquine concentrations were measured,
2017 - Environmental Ordination of Filamentous Bacteria in Activated SludgeWALEBUBLÉ
Reference:
Zornoza, A., Serrano, S. and Alonso, J.L. (2017) Environmental Ordination of Filamentous Bacteria in Activated Sludge. In: Abstracts of the 7th congress of European microbiologists FEMS 2017, Valencia, Spain, 9-13 July 2017.
ABSTRACT- Comparative toxicity of Nerium indicum latex powder (NILP) was studied against two common predatory and weed fishes of different body size in laboratory condition and cemented and muddy pond. LC50 of NILP for predatory fish Channa punctatus was 2.13 mg/L, 7.80 mg/L and 19.26 mg/L to small size, middle size and large size fish respectively in laboratory condition after 24 h exposure periods. Similar trends were also observed in case of cemented and muddy ponds but doses were 1-4 times higher than laboratory conditions. Similar trend of result was also observed against weed fish Mystus mystus. No further mortality was observed beyond 24 h in all set of experiments suggested fast degradation of the toxicant in water.
Key-words- Toxicity, Fish, Botanicals, Nerium indicum, Piscicidal Activity
Method Development and Validation of Tetracycline Antibiotics and their Epime...Dr. Mukesh Raikwar
This document describes the development and validation of a method to detect tetracycline antibiotics and their epimers in marine products according to EU guidelines. The method uses HPLC and LC/MS/MS to separate and detect 7 tetracyclines (tetracycline, oxytetracycline, doxytetracycline, chlortetracycline, and their epimers) in shrimp samples. Validation showed good linearity, recovery, specificity, and other parameters within EU limits. The method provides a simple way to reliably detect tetracycline residues in marine products at the MRL level set by the EU.
Expt. 7 Bioassay of acetylcholine using rat ileum by four point bioassayVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 2 Bioassay of acetylcholine using rat ileum by four point bioassayVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Response of aquatic fern(Azolla), to watercontaminationKavitha Cingam
The document summarizes a study on the response of the aquatic fern Azolla to contamination by the antibiotic ciprofloxacin (Cipro). The study investigated Azolla's ability to uptake Cipro and the effects on its nitrogen-fixing process. The results showed that Azolla is able to uptake concentrations of Cipro above levels that classify plants as hyperaccumulators. Exposure to Cipro negatively impacted the heterocyst/vegetative cell ratio, nitrogenase activity, total nitrogen, and disrupted photosynthesis. This suggests that Cipro is toxic not only to the nitrogen-fixing cyanobacteria in Azolla but also to Azolla itself, demonstrating its potential use in phytoremediation of Cipro contamination
Application of Plasma in Food and AgricultureSITHUHan3
This document summarizes research on using cold plasma in agriculture. It discusses how cold plasma can be used to:
1) Decontaminate seeds by reducing fungal and bacterial counts on seed surfaces through plasma exposure, achieving log reductions in microbes (Section 8.2).
2) Enhance seed germination by accelerating germination rates and increasing sprout lengths through plasma-induced changes to seed coat permeability and plant hormone levels (Section 8.3).
3) Positively impact plant growth by producing reactive oxygen and nitrogen species through plasma that increase nutrient uptake and growth factors when plants are irrigated with plasma-treated water (Section 8.4).
This document summarizes a study that investigated the cytotoxic activity of a chloroform extract and four diterpenes isolated from Salvia ballotiflora against five cancer cell lines. The extract and isolated compounds were tested using an MTT assay to determine their IC50 values. 19-Deoxyisoicetexone had the greatest effect on HeLa cells with an IC50 of 3.2 μg/ml, while the chloroform extract showed the best cytotoxicity against A549 cells with an IC50 of 2.29 μg/ml. These effects were similar to the IC50 values of cisplatin in the respective cell lines. The study isolated and identified the active compounds from S. ballotiflora
IJRET : International Journal of Research in Engineering and Technology is an international peer reviewed, online journal published by eSAT Publishing House for the enhancement of research in various disciplines of Engineering and Technology. The aim and scope of the journal is to provide an academic medium and an important reference for the advancement and dissemination of research results that support high-level learning, teaching and research in the fields of Engineering and Technology. We bring together Scientists, Academician, Field Engineers, Scholars and Students of related fields of Engineering and Technology
This document discusses a study on the effects of herbivory by brant geese and algal blooms on a Zostera marina seagrass community. The study used a factorial experiment to simulate different combinations of clipping/herbivory, algal additions, and nutrient additions. Results showed that clipping significantly reduced seagrass biomass and cover but did not affect its nutrient content. Clipping enhanced seagrass growth after treatment. Algal additions did not affect seagrass directly but a negative relationship was found between green macroalgae and seagrass biomass. Epiphyte biomass on seagrass was also reduced by clipping. Environmental factors like light and
Effect of Temperature on Stream Fish Energetics and Tolerance to Increasing U...Marylou Moore
ABSTRACT
Urban development threatens stream systems throughout the world and has been shown to correspond with predictable changes in fish assemblage attributes. These shifts in fish communities suggests that there may be species specific differences in tolerance to altered environmental conditions related to urbanization. Over the last three decades, a great amount of literature has focused on the development of community-level metrics for assessing biotic integrity of fishes within flowing waters. While these efforts have spawned a great deal of information on community and guild-level responses by stream fishes, it is unknown how individual species’ evolutionary adaptation and physiological acclimation correspond to their ability to tolerate drastic environmental change. This study investigated temperature-dependent responses of metabolism and thermal sensitivity in redbreast sunfish (Lepomis auritus) collected from sites representative of the lowest and highest degree of urban land use throughout the Etowah Watershed, Georgia. Standard metabolic rate (SMR) was shown to be significantly lower in fish drawn from the urban stream at both experimental temperatures (20 °C and 25 °C). Additionally, the urban population had a significantly higher metabolic scope at 25 °C than the non-urban population. These findings support the hypothesis that intraspecific differences in thermal tolerance may exist at localized scales within a small watershed. This study also provides the first known reports of the metabolic rate and aerobic scope of L. auritus.
This document discusses the effects of foliar application of liquid organic fertilizers on crop growth and quality. It begins with introducing liquid organic fertilizers and their benefits over solid organic fertilizers. The objectives are to understand the effects on crop growth, yield, and quality. Several tables show results of research experiments applying different liquid organic fertilizers like vermicompost wash, cow dung wash, and panchagavya on various crops like chickpeas, soybeans, and groundnuts. The results demonstrate increased plant growth parameters, yields, and quality from foliar application of liquid organic fertilizers compared to control treatments.
The extract of Vismia mexicana leaves was found to inhibit HIV-1. Two anthraquinones, vismiaquinone D and vismiaquinone, were isolated from the extract. The structures of the two compounds were determined by NMR and X-ray crystallography. Neither compound showed inhibition of HIV-1 reverse transcriptase, suggesting other compounds in the extract are responsible for the antiviral activity.
This document summarizes a new method for detecting human hormones in drinking water supplies using solid-phase extraction and liquid chromatography-mass spectrometry. The method monitors 9 hormones including estrogens and androgens. Hormones are extracted from water samples using SPE cartridges, separated via HPLC, and identified/quantified on a triple quadrupole mass spectrometer. Recoveries for the hormones from drinking water ranged from 90-124%, and the method provides well-differentiated mass spectrometry peaks for each hormone. The new method complies with EPA Method 539 for monitoring hormones in drinking water supplies.
This document summarizes an experiment evaluating the tolerance of potato genotypes to osmotic stress conditions in vitro. Forty-two potato genotypes from international centers and local varieties were screened under normal conditions and two levels of osmotic stress induced by sorbitol. Various drought tolerance traits such as shoot and root growth, biomass, and proline content were measured. Most traits were significantly affected by genotype, sorbitol treatment, and their interaction. Several genotypes performed relatively better under stress and were identified as potentially drought tolerant for further evaluation under field conditions.
This document summarizes a research study that developed a colorimetric assay using gold nanoparticles to detect beta-agonists. The assay can quickly detect beta-agonists and their analogs in serum, urine, and other liquid samples. The researchers determined the optimal conditions for detection and pretreated different liquid samples. They tested 13 types of beta-agonists in serum, urine, and milk samples and achieved detection limits down to 0.01 ng/mL. The colorimetric assay provides a fast, broad-spectrum method for screening beta-agonists in various samples with sensitivity comparable or better than other methods.
Dielectric parameters of ayurvedic medicines biological approacheSAT Journals
Abstract
The study of dielectric properties of biological materials is of great assistance in exploring the molecular structure and dynamics
of condensed matter. Time domain reflectometry in reflection mode has been used as a technique. A nonlinear least squares fit
method have been used to obtain dielectric parameters viz., static dielectric constant (ɛ0 ) and dielectric relaxation time (τ) at
temperatures 100C, 200C, 300C and 400C of Ayurvedic Medicines.
Keywords: Medicinal plants, Asava, Ethanol,Time domain reflectometry, Fourier transformation, Dielectric
constant, Relaxation time.
This document contains abstracts from two veterinary emergency and critical care conferences that took place in 2014: the International Veterinary Emergency and Critical Care Symposium (IVECCS) in Indianapolis, USA and the European Veterinary Emergency and Critical Care Society (EVECCS) Annual Congress in Prague, Czech Republic. The abstracts cover a range of topics including studies evaluating new diagnostic tests and treatments for emergency and critical care patients. They are organized by conference and then alphabetically by presenter within each small and large animal section.
STUDIES ON THE CHRONIC TOXICITY OF CHLOROQUINE 1948thierry jolaine
This document summarizes a study on the chronic toxicity of chloroquine, an antimalarial drug. In the study:
1) Two groups of 20 subjects each were given different dosage schedules of chloroquine for one year to study long-term toxicity - one group received 0.3 grams daily for 77 days then 0.5 grams weekly, the other received 0.5 grams weekly the whole time.
2) Toxic effects observed included visual difficulties, hair bleaching in blondes, mild skin eruptions in two subjects, slight weight loss, and temporary electrocardiogram changes in the daily dosage group.
3) Plasma chloroquine concentrations were measured,
2017 - Environmental Ordination of Filamentous Bacteria in Activated SludgeWALEBUBLÉ
Reference:
Zornoza, A., Serrano, S. and Alonso, J.L. (2017) Environmental Ordination of Filamentous Bacteria in Activated Sludge. In: Abstracts of the 7th congress of European microbiologists FEMS 2017, Valencia, Spain, 9-13 July 2017.
ABSTRACT- Comparative toxicity of Nerium indicum latex powder (NILP) was studied against two common predatory and weed fishes of different body size in laboratory condition and cemented and muddy pond. LC50 of NILP for predatory fish Channa punctatus was 2.13 mg/L, 7.80 mg/L and 19.26 mg/L to small size, middle size and large size fish respectively in laboratory condition after 24 h exposure periods. Similar trends were also observed in case of cemented and muddy ponds but doses were 1-4 times higher than laboratory conditions. Similar trend of result was also observed against weed fish Mystus mystus. No further mortality was observed beyond 24 h in all set of experiments suggested fast degradation of the toxicant in water.
Key-words- Toxicity, Fish, Botanicals, Nerium indicum, Piscicidal Activity
Method Development and Validation of Tetracycline Antibiotics and their Epime...Dr. Mukesh Raikwar
This document describes the development and validation of a method to detect tetracycline antibiotics and their epimers in marine products according to EU guidelines. The method uses HPLC and LC/MS/MS to separate and detect 7 tetracyclines (tetracycline, oxytetracycline, doxytetracycline, chlortetracycline, and their epimers) in shrimp samples. Validation showed good linearity, recovery, specificity, and other parameters within EU limits. The method provides a simple way to reliably detect tetracycline residues in marine products at the MRL level set by the EU.
Method Development and Validation of Tetracycline Antibiotics and their Epime...
Masters thesis
1. The Effects of Triclosan on Five Marine Algae Species Melanie N. McHenry Department of Biology, Marine Science Program, Jackson State University, P.O. Box 18540, 1400 J.R. Lynch Street, Jackson, Mississippi, USA
2.
3.
4.
5.
6.
7.
8. Background Information Cont. Triclosan Levels in Waste Water Waste water Levels ( µ g/L) Source Primary Effluent 3.4 - 8.0 McAvoy, DC et al. 2002 Final Effluent: Activated Sludge Treatment 0.20 - 0.41 Weigel S. et al. : 2004 Trickling Filter Treatment 1.6 - 2.7 Switzerland Effluents 42 - 213 mg/L Balmer 2005
9.
10. Algae Used in this Study Tetraselmis chuii Nannochloropsis oculata Chaetocerus gracilis Isochrysis galbana Rhodomonas salina
12. The Effects of Triclosan on Various Organisms (Balmer 2005, Russel 2004, Orvos et al. 2002) Organism L(E) 50 Algae Freshwater algae Anabaena flos-aquae 0.97 µg/L Scenedesmus subspicatus 2. 8 µg/L Selenastrum capricornutum 4.46 µg/L Navicula pelliculosa 19.1 µg/L Marine Algae Skeletonema costatum > 66.0 µg/L Fish Danio rerio and Oryzais latipes < 300 µg/L Pimephales promelas 260 µg/L Lepomis macrochirus 370 µg/L Seamonkeys Artemia 2000 µg/L Bacteria Methicillin-resistant Staphylococci 0.1 – 2 mg/L E. coli and K. pueumoniae 5,000 µg/L
13.
14. Experimental Design Treatment (ug/L) Replicate # Temperature (average) Salinity Light (lux) Control 0 3 25 o C 25ppt 2,200 1 0.2 3 25 o C 25ppt 2,200 2 1.0 3 25 o C 25ppt 2,200 3 5.0 3 25 o C 25ppt 2,200 4 25.0 3 25 o C 25ppt 2,200 5 125 3 25 o C 25ppt 2,200 6 250 3 25 o C 25ppt 2,200 7 500 3 25 o C 25ppt 2,200 Solvent Control 7.2% Ethyl Alcohol 3 25 o C 25ppt 2,200
24. Isochrysis growth rate ANOVA table 24 hours Source of Variation Degrees of Freedom Sum of Squares Mean Square F-Value Pr > F Model 10 36.61077037 3.66107704 369.77 < 0.0001 Error 16 0.15841481 0.00990093 Total 26 36.76918519 R-Square Coefficient Var Root MSE YIELD Mean 0.995692 13.61679 0.099503 0.730741
25. Means with the same letter are not significantly different t Grouping Mean N Treatment A 1.85667 3 Control B 1.65000 3 Treatment 1 C 1.29333 3 Treatment 2 D 1.02333 3 Treatment 3 E 0.61333 3 Treatment 4 F 0.31000 3 Treatment 5 G -0.01000 3 Treatment 6 H -203333 3 Treatment 7 A 1.87333 3 Treatment 8
26. Isochrysis growth rate ANOVA table 48 hours Source of Variation Degrees of Freedom Sum of Squares Mean Square F-Value Pr > F Model 10 8.75095556 0.87509556 198.57 < 0.0001 Error 16 0.07051111 0.00440694 Total 26 8.82146667 R-Square Coefficient Var Root MSE YIELD Mean 0.992007 12.11893 0.066385 0.547778
27. Means with the same letter are not significantly different t Grouping Mean N Treatment A 1.16667 3 Control B 0.91000 3 Treatment 1 C 0.72000 3 Treatment 2 D 0.57667 3 Treatment 3 E D 0.49000 3 Treatment 4 E 0.42333 3 Treatment 5 F 0.23667 3 Treatment 6 G -0.8000 3 Treatment 7 A 1.20667 3 Treatment 8
28. Isochrysis growth rate ANOVA table 72 hours Source of Variation Degrees of Freedom Sum of Squares Mean Square F-Value Pr > F Model 10 2.49982222 0.24998222 1097.48 <0.0001 Error 16 0.00364444 0.00022778 Total 26 2.50346667 R-Square Coefficient Var Root MSE YIELD Mean 0.998544 2.538893 0.015092 0.594444
29. Means with the same letter are not significantly different t Grouping Mean N Treatment A 0.9600 3 Control B 0.8500 3 Treatment 1 C 0.82333 3 Treatment 2 D 0.75667 3 Treatment 3 E 0.67333 3 Treatment 4 F 0.56667 3 Treatment 5 G 0.48667 3 Treatment 6 H 0.31667 3 Treatment 7 I -0.08333 3 Treatment 8
30. Isochrysis growth rate ANOVA table 96 hours Source of Variation Degrees of Freedom Sum of Squares Mean Square F-Value Pr > F Model 10 1.23659259 0.12365926 763.15 < <0.0001 Error 16 0.00259259 0.00016204 Total 26 1.23918519 R-Square Coefficient Var Root MSE YIELD Mean 0.997908 2.197527 0.012729 0.579259
31. Means with the same letter are not significantly different t Grouping Mean N Treatment A 0.83333 3 Control A 0.82667 3 Treatment 1 B 0.73333 3 Treatment 2 C 0.67333 3 Treatment 3 D 0.59333 3 Treatment 4 E 0.52667 3 Treatment 5 F 0.48333 3 Treatment 6 G 0.43333 3 Treatment 7 H 0.1100 3 Treatment 8
33. Tetraselmis growth rate ANOVA table 24 hours Source of Variation Degrees of Freedom Sum of Squares Mean Square F-Value Pr > F Model 10 10.17803704 1.01780370 81.79 < 0.0001 Error 16 0.1991037 0.01244398 Total 26 10.37714074 R-Square Coefficient Var Root MSE YIELD Mean 0.980813 55.36618 0.111553 0.201481
34. Means with the same letter are not significantly different t Grouping Mean N Treatment A 0.68000 3 Control B A 0.60667 3 Treatment 1 B 0.51000 3 Treatment 2 B 0.46333 3 Treatment 3 C 0.24333 3 Treatment 4 C 0.14667 3 Treatment 5 D -0.15667 3 Treatment 6 E -1.36667 3 Treatment 7 A 0.68667 3 Treatment 8
35. Tetraselmis growth rate ANOVA table 48 hours Source of Variation Degrees of Freedom Sum of Squares Mean Square F-Value Pr > F Model 10 1.02720000 0.10272000 2.89 0.0287 Error 16 0.56946667 0.03559167 Total 26 1.59666667 R-Square Coefficient Var Root MSE YIELD Mean 0.643340 44.68205 0.188658 0.422222
36. Means with the same letter are not significantly different t Grouping Mean N Treatment A 0.6200 3 Control A 0.5933 3 Treatment 1 B A 0.5567 3 Treatment 2 B A 0.5400 3 Treatment 3 B A 0.4567 3 Treatment 4 B 0.4667 3 Treatment 5 B C 0.2367 3 Treatment 6 C -0.0267 3 Treatment 7 A 0.3467 3 Treatment 8
37. Tetraselmis growth rate ANOVA table 72 hours Source of Variation Degrees of Freedom Sum of Squares Mean Square F-Value Pr > F Model 10 1.05683704 0.10568370 3.38 0.0149 Error 16 0.50061481 0.03128843 Total 26 1.55745185 R-Square Coefficient Var Root MSE YIELD Mean 0.678568 30.71321 0.176885 0.575926
38. Means with the same letter are not significantly different t Grouping Mean N Treatment A 0.8567 3 Control B A 0.7700 3 Treatment 1 B A 0.6933 3 Treatment 2 B A C 0.6300 3 Treatment 3 B A C 0.5733 3 Treatment 4 B C 0.5100 3 Treatment 5 C 0.3767 3 Treatment 6 0.1600 3 Treatment 7 A 0.6133 3 Treatment 8
39. Tetraselmis growth rate ANOVA table 96 hours Source of Variation Degrees of Freedom Sum of Squares Mean Square F-Value Pr > F Model 10 0.77628889 0.07762889 3.08 0.0221 Error 16 0.40377778 0.02523611 Total 26 1.18006667 R-Square Coefficient Var Root MSE YIELD Mean 0.657835 21.69543 0.158859 0.73222
40. Means with the same letter are not significantly different t Grouping Mean N Treatment A 0.8967 3 Control A 0.8700 3 Treatment 1 A 0.8500 3 Treatment 2 A 0.8067 3 Treatment 3 A 0.7867 3 Treatment 4 A 0.7433 3 Treatment 5 A 0.6600 3 Treatment 6 A 0.3067 3 Treatment 7 B 0.6700 3 Treatment 8
42. Nannochloropsis growth rate ANOVA table 24 hours Source of Variation Degrees of Freedom Sum of Squares Mean Square F-Value Pr > F Model 10 5.89219259 0.58921926 45.75 < 0.0001 Error 16 0.20605926 0.0128787 Total 26 6.09825185 R-Square Coefficient Var Root MSE YIELD Mean 0.966210 17.30140 0.113484 0.655926
43. Means with the same letter are not significantly different t Grouping Mean N Treatment A 1.32667 3 Control B 0.92667 3 Treatment 1 C B 0.85000 3 Treatment 2 C 0.71000 3 Treatment 3 D 0.51333 3 Treatment 4 E 0.29333 3 Treatment 5 F -0.08333 3 Treatment 6 F 0.09333 3 Treatment 7 A 1.27333 3 Treatment 8
44. Nannochloropsis growth rate ANOVA table 48 hours Source of Variation Degrees of Freedom Sum of Squares Mean Square F-Value Pr > F Model 10 4.89813333 0.48981333 4.00 0.0069 Error 16 1.95913333 0.12244583 Total 26 6.85726667 R-Square Coefficient Var Root MSE YIELD Mean 0.714298 32.77111 0.349923 1.067778
45. Means with the same letter are not significantly different t Grouping Mean N Treatment A 1.6300 3 Control B A 1.5067 3 Treatment 1 B A 1.3900 3 Treatment 2 B A C 1.1600 3 Treatment 3 B A C 1.1033 3 Treatment 4 B C 0.9500 3 Treatment 5 C 0.5600 3 Treatment 6 0.2100 3 Treatment 7 A 1.1000 3 Treatment 8
46. Nannochloropsis growth rate ANOVA table 76 hours Source of Variation Degrees of Freedom Sum of Squares Mean Square F-Value Pr > F Model 10 4.89813333 0.48981333 4.00 0.0069 Error 16 1.95913333 0.12244583 Total 26 6.85726667 R-Square Coefficient Var Root MSE YIELD Mean 0.714298 32.77111 0.349923 1.067778
47. Means with the same letter are not significantly different t Grouping Mean N Treatment 1.63000 3 Control B 1.5067 3 Treatment 1 B 1.3900 3 Treatment 2 B C 1.1600 3 Treatment 3 B C 1.1033 3 Treatment 4 B C 1.1000 3 Treatment 5 D C 0.9500 3 Treatment 6 D 0.5600 3 Treatment 7 B A C 0.2100 3 Treatment 8
48. Nannochloropsis growth rate ANOVA table 96 hours Source of Variation Degrees of Freedom Sum of Squares Mean Square F-Value Pr > F Model 10 2.04006667 0.20400667 20.12 <0.0001 Error 16 0.16220000 0.01013750 Total 26 2.20226667 R-Square Coefficient Var Root MSE YIELD Mean 0.926349 19.52945 0.100685 0.515556
49. Means with the same letter are not significantly different t Grouping Mean N Treatment A 0.73667 3 Control B A 0.7100 3 Treatment 1 B A 0.66333 3 Treatment 2 B A 0.63000 3 Treatment 3 B C 0.54667 3 Treatment 4 C 0.41000 3 Treatment 5 D 0.23333 3 Treatment 6 E -0.06333 3 Treatment 7 A 0.77333 3 Treatment 8
51. Rhodomonas growth rate ANOVA table 24 hours Source of Variation Degrees of Freedom Sum of Squares Mean Square F-Value Pr > F Model 10 3.28800000 0.32880000 31.99 <0.0001 Error 16 0.16446667 0.01027917 Total 26 3.45246667 R-Square Coefficient Var Root MSE YIELD Mean 0.952363 12.48257 0.101386 0.812222
52. Means with the same letter are not significantly different t Grouping Mean N Treatment A 1.13667 3 Control B A 1.09333 3 Treatment 1 B C 0.95333 3 Treatment 2 C 0.91667 3 Treatment 3 D C 0.78667 3 Treatment 4 D 0.69333 3 Treatment 5 E 0.34667 3 Treatment 6 F 0.14333 3 Treatment 7 A 1.24000 3 Treatment 8
53. Rhodomonas growth rate ANOVA table 48 hours Source of Variation Degrees of Freedom Sum of Squares Mean Square F-Value Pr > F Model 10 1.68814815 0.16881481 2.29 0.0675 Error 16 1.17988148 0.07374259 Total 26 2.86802963 R-Square Coefficient Var Root MSE YIELD Mean 0.588609 35.55775 0.271556 0.763704
54. Means with the same letter are not significantly different t Grouping Mean N Treatment A 1.1267 3 Control A 1.05333 3 Treatment 1 A 0.7267 3 Treatment 2 B A 0.8233 3 Treatment 3 B A 0.7600 3 Treatment 4 B A 0.7267 3 Treatment 5 B C 0.5567 3 Treatment 6 C 0.2333 3 Treatment 7 B A 0.8667 3 Treatment 8
55. Rhodomonas growth rate ANOVA table 72 hours Source of Variation Degrees of Freedom Sum of Squares Mean Square F-Value Pr > F Model 10 1.36481481 0.13648148 3.80 0.0087 Error 16 0.57394815 0.03587175 Total 26 1.93876296 R-Square Coefficient Var Root MSE YIELD Mean 0.703962 27.97460 0.189398 0.677037
56. Means with the same letter are not significantly different t Grouping Mean N Treatment A 0.9300 3 Control A 0.8933 3 Treatment 1 A 0.8367 3 Treatment 2 A 0.8100 3 Treatment 3 B A 0.7167 3 Treatment 4 B A 0.6300 3 Treatment 5 B C 0.4567 3 Treatment 6 C 0.1800 3 Treatment 7 B A 0.6400 3 Treatment 8
57. Rhodomonas growth rate ANOVA table 96 hours Source of Variation Degrees of Freedom Sum of Squares Mean Square F-Value Pr > F Model 10 0.97977037 0.09797704 3.17 0.0196 Error 16 0.49503704 0.03093981 Total 26 1.47480741 R-Square Coefficient Var Root MSE YIELD Mean 0.664338 29.90695 0.175897 0.599148
58. Means with the same letter are not significantly different t Grouping Mean N Treatment A 0.8600 3 Control B A 0.7567 3 Treatment 1 B A C 0.6900 3 Treatment 2 B A C 0.6567 3 Treatment 3 B A C 0.6000 3 Treatment 4 B C 0.5467 3 Treatment 5 D C 0.4167 3 Treatment 6 D 0.1667 3 Treatment 7 B A C 0.6000 3 Treatment 8
60. Chaetocerus growth rate ANOVA table 24 hours Source of Variation Degrees of Freedom Sum of Squares Mean Square F-Value Pr > F Model 10 35.12774815 3.51277481 34.51 < 0.0001 Error 16 1.62859259 0.010178704 Total 26 36.75634074 R-Square Coefficient Var Root MSE YIELD Mean 0.955692 32.94112 0.319041 0.968519
61. Means with the same letter are not significantly different t Grouping Mean N Treatment A 2.0967 3 Control B A 1.7767 3 Treatment 1 B A 1.5867 3 Treatment 2 B C 1.2633 3 Treatment 3 D C 0.9100 3 Treatment 4 D 0.6700 3 Treatment 5 E 0.0433 3 Treatment 6 F -1.6967 3 Treatment 7 A 2.0667 3 Treatment 8
62. Chaetocerus growth rate ANOVA table 48 hours Source of Variation Degrees of Freedom Sum of Squares Mean Square F-Value Pr > F Model 10 4.85472593 0.48547259 2.86 0.0298 Error 16 2.71705926 0.16981620 Total 26 7.57178519 R-Square Coefficient Var Root MSE YIELD Mean 0.641160 50.82853 0.412088 0.810741
63. Means with the same letter are not significantly different t Grouping Mean N Treatment A 1.3067 3 Control B A 1.1767 3 Treatment 1 B A 1.0633 3 Treatment 2 B A 0.9633 3 Treatment 3 B A 0.9000 3 Treatment 4 B A 0.8033 3 Treatment 5 B 0.5367 3 Treatment 6 C -0.2133 3 Treatment 7 B A 0.7600 3 Treatment 8
64. Chaetocerus growth rate ANOVA table 72 hours Source of Variation Degrees of Freedom Sum of Squares Mean Square F-Value Pr > F Model 10 1.06761482 0.10676148 3.04 0.0233 Error 16 0.56248148 0.03515509 Total 26 1.63009630 R-Square Coefficient Var Root MSE YIELD Mean 0.654940 29.31336 0.187497 0.639630
65. Means with the same letter are not significantly different t Grouping Mean N Treatment A 0.9167 3 Control B A 0.8300 3 Treatment 1 B A 0.7700 3 Treatment 2 B A 0.6967 3 Treatment 3 B A 0.6500 3 Treatment 4 B A 0.6200 3 Treatment 5 B 0.5600 3 Treatment 6 C 0.5233 3 Treatment 7 B 0.5600 3 Treatment 8
66. Chaetocerus growth rate ANOVA table 96 hour Source of Variation Degrees of Freedom Sum of Squares Mean Square F-Value Pr > F Model 10 0.65040000 0.06504000 2.63 0.0412 Error 16 0.39600000 0.02475000 Total 26 1.04640000 R-Square Coefficient Var Root MSE YIELD Mean 0.621560 27.76259 0.157321 0.56667
67. Means with the same letter are not significantly different t Grouping Mean N Treatment A 0.7800 3 Control B A 0.7433 3 Treatment 1 B A C 0.6733 3 Treatment 2 B A C 0.5967 3 Treatment 3 B A C 0.5533 3 Treatment 4 B D C 0.5033 3 Treatment 5 D C 0.4500 3 Treatment 6 D 0.2367 3 Treatment 7 B A C 0.5633 3 Treatment 8
82. The time period and its corresponding EC 50 value Time Period EC 50 value Control (0µg/L) 109 μg/mL Treatment 1 (0.2µg/L) 98 μg/mL Treatment 2 (1.0µg/L) 168 μg/mL Treatment 3 (5.0µg/L) 128 μg/mL
84. The time period and its corresponding EC 50 value Time Period EC 50 value Control (0µg/L) 46.2 μg/mL Treatment 1 (0.2µg/L) 68 μg/mL Treatment 2 (1.0µg/L) 183 μg/mL Treatment 3 (5.0µg/L) 162 μg/mL
94. Acknowledgements This research was supported, in part, by a grant from the National Oceanic and Atmospheric Administration grant # NA17AE1626, Subcontract # 27-0629-017, through the Environmental Cooperative Science Center at Florida A&M University to Jackson State University. M. McHenry was supported through the National Institutes of Health funded Research Initiative for Scientific Enhancement (RISE) grant awarded to Jackson State University.