Ready-to-eat Vegetables as new sources of contamination-infection of Pseudomo...Sardegna Ricerche
Alessandra Scano presenta alcuni aspetti del progetto RealTime Check IV Gamma, il progetto finanziato da Sardegna Ricerche con i fondi del POR FESR Sardegna 2014-2020 e gestito dall'Istituto di Scienze delle Produzioni Alimentari (ISPA) del CNR, in collaborazione con il Dipartimento di Scienze chirurgiche dell'Università degli Studi di Cagliari.
Il progetto ha l’obiettivo di fornire alle imprese sarde che operano nel settore della IV Gamma due dispositivi per il monitoraggio rapido della qualità e dell'idoneità igienico-sanitaria di prodotti ortofrutticoli minimamente trasformati.
Evaluation of pseudomonas koreinsis qfr5 for plant pathogenAnamika Rana
Identified strain QFR5 is Pseudomonas koriensis.
Inoculation of qrf5 strain displayed great antagonistic potential against four phyto pathogens and enhanced plant biomass of D.sissoo cultivar.
Disease incidence was found less in case of PS90 cultivar as compared to PS52 when inoculated with QFR-5 strain.
Ready-to-eat Vegetables as new sources of contamination-infection of Pseudomo...Sardegna Ricerche
Alessandra Scano presenta alcuni aspetti del progetto RealTime Check IV Gamma, il progetto finanziato da Sardegna Ricerche con i fondi del POR FESR Sardegna 2014-2020 e gestito dall'Istituto di Scienze delle Produzioni Alimentari (ISPA) del CNR, in collaborazione con il Dipartimento di Scienze chirurgiche dell'Università degli Studi di Cagliari.
Il progetto ha l’obiettivo di fornire alle imprese sarde che operano nel settore della IV Gamma due dispositivi per il monitoraggio rapido della qualità e dell'idoneità igienico-sanitaria di prodotti ortofrutticoli minimamente trasformati.
Evaluation of pseudomonas koreinsis qfr5 for plant pathogenAnamika Rana
Identified strain QFR5 is Pseudomonas koriensis.
Inoculation of qrf5 strain displayed great antagonistic potential against four phyto pathogens and enhanced plant biomass of D.sissoo cultivar.
Disease incidence was found less in case of PS90 cultivar as compared to PS52 when inoculated with QFR-5 strain.
Isolation and Identification of Avibacteriumparagallinarum from Layer Chicken...Dr. Md. Ehsanul Haque
An investigation was conducted for isolation, identification and determination of antibiotic sensitivity of Avibacteriumparagallinarun, the causal agent of infectious coryza, from layer chickens. A total of 21 samples with characteristic symptoms of the disease were collected from a Hatchery of Gazipur. Tissue specimens obtained aseptically from swollen infra orbital sinus and tracheal swab were processed, of which, 3 were found positive while the rest 18 were negative. Isolation of bacteria was performed by first putting the specimen in Nicotinamide adenine dinucleotide (NAD) enriched phosphate buffer broth, anaerobically incubated for 24 hours followed by culturing loopful of broth on Blood agar (BA) and Chocolate agar (CA) plates enriched with NAD and streaked with feeder organism of Staphylococcus. aureus. On 24 hours of anaerobic incubation (candle jar method), dew drop satellite colonies of A. paragallinarum were visible on the culture plates. Cultural characteristics of bacteria as well as their staining, morphological, motility and biochemical properties such as sugar fermentation, MR and V-P tests, Indole production and catalase tests were recorded for identification. Further, antibiogram study revealed that the isolates were sensitive to Ciprofloxacin, Chloramphenicol and Gentamicin but resistant to Ampicillin, Amoxycillin, Oxytetracycline, Erythromycin and Sulphamethoxazole.
Isolation and Identification of Avibacteriumparagallinarum from Layer Chicken...Dr. Md. Ehsanul Haque
An investigation was conducted for isolation, identification and determination of antibiotic sensitivity of Avibacteriumparagallinarun, the causal agent of infectious coryza, from layer chickens. A total of 21 samples with characteristic symptoms of the disease were collected from a Hatchery of Gazipur. Tissue specimens obtained aseptically from swollen infra orbital sinus and tracheal swab were processed, of which, 3 were found positive while the rest 18 were negative. Isolation of bacteria was performed by first putting the specimen in Nicotinamide adenine dinucleotide (NAD) enriched phosphate buffer broth, anaerobically incubated for 24 hours followed by culturing loopful of broth on Blood agar (BA) and Chocolate agar (CA) plates enriched with NAD and streaked with feeder organism of Staphylococcus. aureus. On 24 hours of anaerobic incubation (candle jar method), dew drop satellite colonies of A. paragallinarum were visible on the culture plates. Cultural characteristics of bacteria as well as their staining, morphological, motility and biochemical properties such as sugar fermentation, MR and V-P tests, Indole production and catalase tests were recorded for identification. Further, antibiogram study revealed that the isolates were sensitive to Ciprofloxacin, Chloramphenicol and Gentamicin but resistant to Ampicillin, Amoxycillin, Oxytetracycline, Erythromycin and Sulphamethoxazole.
Production of African Cassava Mosaic Virus (ACMV) Specific Polyclonal Antibod...iosrjce
Serological techniques are commonly used in the detection and characterization of plant viruses.
These methods employ the use of antisera produced by highly purified preparations in intramuscular,
intradermal and intraocular. In this study oral route was explored using crude extracts. Two groups (control
and experimental) of Swiss albino mice consisting of two replicates were immunized via the oral route with
crude extracts from uninfected cassava plants (Manihot esculenta) and cassava plants systematically infected
with African Cassava Mosaic Virus (ACMV). Uninfected and infected leaves were grinded separately in saline
solution (0.15M) at 1:2 (w/v) with laboratory mortar and pestle and then filtered with double layered cheese
cloth of 75µm to obtain extracts. Clarified extracts were orally administered to the mice in daily doses of 200µl
per mice for 21 days and booster doses were also given at day 28 and 35 respectively. Antiserum were obtained
from the mice for 6 consecutive weeks after the commencement of immunization and were analyzed using
antigen coated plate (ACP) and triple antibody sandwich (TAS) indirect enzyme- linked immunosorbent assay
(ELISA). Group A antisera gave negative reactions (OD values < 1.5) while group B antisera reacted positively
(OD values ≥ 1.5) in the two methods used. The polyclonal antisera obtained were very specific to ACMV in
ACP and TAS ELISA. This appears to be the first antisera specific to ACMV obtained by oral immunization of
mice. Oral immunization is considered less stressful for animals, the method is a fast, simple and cheap way for
producing antisera to plant virus compared to the traditional methods of using purified preparations for
immunization. We have used this procedure in the production of antisera yet there is room for improvement in
immunization strategies to enhance antibody production. Immunization dosage can also be tried and
manipulated in bigger animals like rabbits and chicken. This research work leaves room for further exploration
of similar procedure in bigger experimental animals like rabbits and chicken for greater antiserum production.
The Gram-negative A. actinomycetemcomitans is assumed to be the primary etiologic agent of LAgP and has also been implicated in chronic periodontitis and severe non-oral infections.
Bacterial Conjugation ProjectAn Abstract of 200 words or less that.pdffashiondestinationld
Bacterial Conjugation Project
An Abstract of 200 words or less that summarizes the goal/ hypothesis, methods, results, and
implications/conclusions from this study
In this lab we will be using the bacterium Eschericha coli as our research organism. When using
bacteria and other microbes, it is very important that sterile technique is used to make sure that
you are working with pure cultures of the research organism, and that the organisms remain
confined to your culture containers. Take care to prevent contamination of the culture with other
microbes that may be present in the air or on lab benches, people, or equipment. Avoid
contacting the bacteria yourself. The organisms used in this lab are not pathogenic, but it is
important to learn safe habits. The following precautions should be strictly followed in the
interests of safety and good science.
Solution
Bacterial conjugation is a horizontal genen transfer process from a donor cell bearing one or
more conjugative plasmids to a plasmid free recipient cell.Conjugative plasmids in most bacteria
can even be transferred in distantly related microorganisms ,for this reason antibiotic resistance
can spread among pathogenic bacteria. Conjugal plasmid transfer in both Gram positive and
Gram negative bacteria requires close physical contact between mating cells and is usually
encoded by plasid encoded proteins which provide tra genes.Conjugation was first discovered by
Lederberg and Tatum in 1946.
Conjugation involves special type of replication in which plasmid DNA molecule replicates
during conjugation,with one copy remaing in the donor cell and the other being transferred to the
recipient.
Below is a small experiment to demonstrate conjugation
The basic conjugative donor strain is E.coli S17-1 containing the tra gene in the chromosome.
The recipient strain of E.coli should be resistant to naladixic acid.The overnight culture of donor
and recipient strain are diluted 10 fold. After growing ovrnight at 37. C, colonies on the plates
are counted. Abot 100 microlitre of donor cells are mixed with 100 microlitre of recipient cells.
The mixture is subjected to shaking and the spread on LB agar containing naladixic acid and a
selectable marker kanamycin or ampicillin.Plates are incubated at 37.C.3-5 colonies of
transconjugants are selected to confirm plasmid carriage by agarose gel eectrophoresis..
1. Conjugative plasmids are contributing to the resistome of quagga mussels
Kylli Paavola
Marquette University
Program Mentor: Dr. Krassimira Hristova
Antibiotic resistant bacteria (ARB) and genes (ARG) occur naturally in the
environment and their presence can be increased by antibiotic pollution. Invasive
mussel species that reside in the Milwaukee harbor receive this pollution resulting
in the accumulation and release of ARB and conjugative plasmids from their gut. A
laboratory feeding experiment was designed to show the uptake and depuration
rate of E. coli CV601gfp into the gut of the mussel. Plating on TSA plates with
Kanamycin (Km, 100 ng/uL), Rifampicin (Rif, 50ng/uL), and Cyclohexamide
(CXM,100 ng/uL) allowed for the strict isolation of CV601gfp, confirmed by UV.
Utilizing this gfp-labeled strain that has no native plasmids and is not resistant to
the antibiotics tested allowed for the screening of ARG-carrying plasmids from the
mussels microbiome. We found that the gut of the filter feeding mussel harbor
bacteria with conjugative plasmids, and it is also a natural environment that
promotes horizontal gene transfer even with the rapid intake and depuration rates.
In addition, conjugation experiments with E. coli CV601gfp and mussel tissue
bacteria, from mussels collected from Milwaukee Harbor and Little Cedar Lake,
were conducted to test for presence of plasmids with ARG. The conjugation mix was
plated on TSA plates with Km/Rif/CXM and six different antibiotics. Mussels from
the less polluted Little Cedar Lake showed resistance to less antibiotics, than the
mussels from the Milwaukee harbor, most likely due to different levels of lake
pollution and human impact.
