Let&#x27;s Talk Research Annual Conference - 24th-25th September 2014 (Jane Rogan)

Albert Einstein Cancer Center

"Facilitating tissue collection for translational research": Jane Rogan's presentation from the conference.

Healthcare

Facilitating tissue collection for translational research
Jane Rogan
Business Manager

MCRC Biobanking: The Basic Concept
Make quality tissue collection and retrieval easy for the researcher
•Regulatory Framework
•Sample Collection Logistics
•Sample Quality
•Informatics
•Sample Access

Collaborating Trusts
MCRC Biobank
Pennine Acute Hospitals Trust
The Christie NHS Foundation Trust
South Manchester NHS Foundation Trust
Salford Royal NHS Foundation Trust
HPB
Breast
Colorectal Skin Urology Gynaecology Haematological Malignancies
Lung Breast Head & Neck Colorectal
Breast Upper GI Urology Neurology

3%
15%
1%
7%
11%
11%
1%
18%
33%
Collections By Disease Type - Solid Tumours
Brain
Breast
Sarcoma
Skin
Colorectal
Upper GI
Gynae
Urology
Lung
Donations from over 6000 patients

47%
11%
10%
32%
Collections By Disease Type - Blood Disorders
AML
B-ALL
MDS
Other
Donations from 400 patients

Biobank Project Applications
1
13
5
4
5
2
11
6
3
1
4
8
23
Gynae
Breast
Colorectal
Upper GI
GU
Lymphoma
Haematology
Lung
Head and neck
Neuro
Melanoma
Mixed Sample Types
QC/Method Development
0
5
10
15
20
25
Total Applications: 86 Total Approved: 75 Average Approval Time: 30 working days (median)

The “Six-Pack”
1.Fresh Frozen Diseased Tissue
2.Fresh Frozen Non-involved Tissue
3.FFPE Disease Tissue
4.FFPE Non-involved Tissue
5.Pre-operative Blood sample
6.Pre-operative Urine sample

Melanoma Tissue Collection Pathway
Collect Fresh From Theatres
Fresh Cut-Up in Pathology
Diagnostic Sample
Research Sample
Mouse model of tumour
Frozen Tissue for Nucleic Acid Extraction
Fixed Tissue for Staining

Evolving the Collections
Requirement
Considerations
Prospective Fresh Tissue
•Availability of tissue
•Staff available for sample delivery
•Media for keeping samples viable
Use for Animal Models
•Specific consent for animal models
•Size of tumour tissue
Alternative Sample Types (fluids, plucked hair)
•Routes / logistics of sample collection
Serial Blood Collection inc CTCs
•Biobank Technician presence in clinic
Past samples (e.g. pre- treatment/primary tumours)
•Specific consent
•Requesting blocks from local hospitals
Sampling tumour in different areas
•Further input from pathology
Clinical Trial Sample Collection
•Space for sample storage
•Different protocols for each trial

Biobank Projects by Type
17
39
10
8
Fresh Tissue
Banked Samples
Archive FFPE/TMA
Prospective Protocol
Total Approved Projects By Type
0
2
4
6
8
10
12
14
16
Fresh Tissue
Banked Samples
Archive FFPE/TMA
Prospective Protocol
Active Projects By Type (Total 33*)

Tissue Microarrays
•Current disease areas include prostate, bladder, lung
•Link to clinical databases
•Recently obtained Section 251 Support which allows the Biobank to link archival samples to clinical data
•Support through Surgical Oncology Research Theme – Data Manager employed

External Projects utilising Biobank Resource
•Stratified Medicine Programme – Phase 2
–Lung focus
–Biopsy samples from late stage patients
•100k Genome Project
–Lung, Colorectal, Prostate
•TracerX
–Facilitating sample collection
•MesoBank
–Mesothelioma

Research Biopsies
•Working with clinical teams to introduce sample collection as a research only procedure
•How tumours change over time
•Comparing primary and metastatic tumours in patients
•Research into why patients are relapsing on targeted treatments
•Pilot to being in Malignant Melanoma - 2014

Key Success Drivers
•Established network model with existing infrastructure and pathways for sample collection
•Excellent clinical engagement across all cancer disease types
•Robust governance framework for tissue and data
•Proven track record of providing quality samples
•Research needs drive the sample collection, not the other way round

Thank you
http://www.mcrc.manchester.ac.uk/biobank/

Cambridge Healthtech Institute is accepting scientific posters for their 2nd Annual FAST Congress on improving drug screening methods using 3D models and phenotypic screening. The deadline to submit an abstract for a poster is October 10, 2014. Posters on topics related to 3D cellular models, phenotypic screening, organ-specific testing, and developing assays for live tissue models are welcome. Presenters will have their abstracts published in the conference proceedings and be entered to win a cash prize.

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