The document provides a comprehensive overview of ImageJ software for microstructure analysis, emphasizing its cross-platform compatibility, open-source nature, and various analytical capabilities. It details functions such as creating selections, measuring areas and lengths, and calibrating images using real-world units. Additionally, it offers step-by-step instructions for specific analyses, including calculating pearlite content and measuring areas.

1. ImageJ Software for
Microstructure Analysis
ENMT604009
Wahyuaji NP
Departemen Teknik Metalurgi dan Material FTUI
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2. ImageJ Software
• Runs Everywhere:
• ImageJ is written in Java, which allows it to run on Linux, Mac OS X and
Windows, in both 32-bit and 64-bit modes.
• Open Source:
• ImageJ and its Java source code are freely available and in the public
domain. No license is required.
• Selections:
• Create rectangular, elliptical or irregular area selections. Create line and
point selections. Edit selections and automatically create them using the
wand tool. Draw, fill, clear, filter or measure selections. Save selections and
transfer them to other images.
• Analysis:
• Measure area, mean, standard deviation, min and max of selection or entire
image. Measure lengths and angles. Use real world measurement units such
as millimeters. Calibrate using density standards. Generate histograms and
profile plots.

3. ImageJ Software
• Download link:
• https://imagej.nih.gov/ij/download.html

4. ImageJ Software
• Ferrite & pearlite microstructure

5. ImageJ Software
• Pearlite content calculation by ImageJ software

6. ImageJ Software
• Image Scale
• Draw a line over scale bar on the micrograph, zoom in if needed
• “Analyze”  “Set Scale”
• Distance in pixels: depend on line length
• Known distance : from scale bar
• Unit: from scale bar
• “OK”

7. ImageJ Software
• Analysis Area
• Draw rectangle over desired area
• “Image”  “Duplicate”  OK
• “Image”  “Type”  “8-bit”
• “Image”  “Adjust”  “Threshold”
• Auto / adjust the slider
• Apply (if you need the contrast)
• If you still need the microstructure image, continue on the next step
without clicking Apply

8. ImageJ Software
• Area Measurement
• “Analyze”  “Set Measurements”
• Select measurement type (e.g. Area & Area Fraction)
• OK
• “Analyze”  “Analyze Particles”
• Set parameters needed
• “Summarize” must be ticked
• Recommended options: “display results”, “add to manager”
• OK
• Check on the “Summary” window
• DONE!