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1. Internship Report – Alkhidmat
Diagnostic Center, Layyah
Ayesha Saleem
Reg# 21-CUVAS-0430
BS Medical Laboratory Technology 2021-2025

2. Introduction
• Duration: 5th Feb 2025 – 5th May 2025
• Supervisor: Dr. Afsan Amir Sumra
• Key Focus Areas:
• Clinical Pathology (Urine/Semen analysis)
• Biochemistry (LFTs, RFTs, Electrolytes)
• Hematology (CBC, PT/APTT, ESR)
• Serology (HBsAg, HCV, HIV, Malaria testing)

3. Phlebotomy & Sample Collection
• Vacutainer Types & Uses:
• Red: Chemistry tests (LFTs, RFTs)
• Lavender: CBC (EDTA anticoagulant)
• Light Blue: Coagulation tests (PT/APTT)
• Key Steps:
Patient ID,
vein puncture,
 proper mixing,
labeling.

4. Hematology Lab Highlights
• CBC (SYSMEX XS-1000i):
Parameters:
– Hb, RBCs, WBCs, Platelets.
Normal Values:
– E.g., Hb (Male: 13.9–16.9 g/dL).
• Coagulation Tests:
PT (11–15 sec),APTT(26–32 sec)
INR for warfarin monitoring.
• ESR (Westergren Method):
• Measures inflammation.

5. Biochemistry Lab Highlights
• Routine Chemistry
Indiko Analyzer:
– Tests: Glucose,
– Uric Acid,
– LFTs (ALT, AST),
– RFTs (Creatinine).
• Electrolytes (Elite Ultra):
Na , K , Cl .
⁺ ⁺ ⁻
• Special Chemistry:
Lifotonic ECL-8000:
– Thyroid Profile (T3, T4, TSH),
– Troponin I.

6. Blood Bank & Urine/Semen Analysis
Blood Grouping:
Forward/reverse typing
(e.g., A+, B+).
Cross matching:
Ensures donor compatibility.
Urine Analysis:
• Physical (color, turbidity)
• Chemical (dipstick),
• Microscopic (WBCs, RBCs).
Semen Analysis:
• Motility, sperm count.

7. Serology Lab Highlights
• Rapid Tests (Manual Kits):
– HBsAg, HCV, HIV: Positive = 2 lines (C + P).
– Malaria Parasite: Detects Plasmodium antigens.
– ASO Test: Strep infection (agglutination =
positive).

8. Key Learnings & Skills
• Operated analyzers (SYSMEX, Indiko,
Lifotonic).
• Performed manual tests (ESR, blood grouping,
serology).
• Learned QC protocols and safety measures.
• Improved teamwork and patient interaction
skills.

9. Conclusion
• Gained hands-on experience in diagnostic
procedures.
• Bridged theoretical knowledge with practical
application.

10. Thank You!