1. HOW IT WORKS: UV/Vis Spectrophotometer Standards
Problem: Having to verify GFS Chemicals also offers
that an instrument is other standards that can be
performing properly and is utilized to validate other
in calibration is becoming aspects of an instrument’s
increasingly common for performance. Wavelength,
regulatory committees and stray light, and resolution
internal QC procedures. filters are available. When
This is particularly true used together with the In-
when using UV/Vis Spec® photometric
spectrophotometers. These standards you can check
instruments can slowly drift the overall health of your
out of spec as the lamp spectrophotometer .
ages. In order to know that The In-Spec®
the data you are collecting used from 200-900nm. product line also offers a
is accurate it is vital to verify These standards are very solution designed
the instrument’s easy to use since they are specifically for the
performance. non-toxic, come completely increasingly popular short
pre-made with no dilutions pathlength (1mm)
Solution: By using a NIST- or mixing required and do instruments. These
traceable standard you can not require special disposal instruments utilize a very
verify that an instrument is procedures when you are short pathlength of light and
in fact performing as it finished using them. GFS small sample usage. The
should. This provides Chemicals offers a broad In-Spec® Nano-Kit consists
validity to the data that is range of absorbance values of two standards to check
collected with the to match your tests or the absorbance values at
instrument. custom values are 260, 280, and 320nm.
Many of the available. These wavelengths are the
solutions that are available To use the In-Spec® most common for DNA/RNA
are either very costly, toxic, standards you would first quantification tests. The kit
or not flexible and can’t baseline the instrument with comes with data for those
meet your exact the supplied Background wavelengths at a 1mm
specifications. GFS Solution then you would run pathlength.
Chemicals however has the the standard. You would When properly used
ability with its In-Spec® compare the values that are and documented, the use of
Standards to find a solution obtained on your instrument a secondary, NIST-
that will better fit your to the values listed on the traceable standard can
requirements. Certificate of Analysis. If provide you the security and
GFS Chemical’s In- the values are within the peace of mind in knowing
Spec® product line has a greater of 5% or +/- 0.004 that the data you collect
broad range of solutions. added to your instruments with your instrument is in
For photometric accuracy state accuracy then your fact accurate.
there are the In-Spec® instrument is performing
standards which can be well.