2011 In-Spec Catalog


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2011 In-Spec Catalog

  1. 1. SpectrophotometricStandards Reference ® Guide NIST Traceable CHEMICALS Customizable ® No Mixing or Diluting
  2. 2. Ordering Information Customer service representatives and technical support are Contents Table of Contents available Monday through Friday from 7:30 am to 4:00 pm Continuous Improvement 4-5 Eastern Time. Orders may be placed by phone, e-mail or fax. Every order receives an order confirmation that includes items In-Spec® UV Standards 5-6 ordered, pricing, shipping method and estimated shipping date. In-Spec® Visible Standards 5-6 GFS Chemicals, Inc. In-Spec® Patent Blue Standards 8 800 Kaderly Drive In-Spec® Amaranth Red Standards 9 Columbus, OH 43228 In-Spec® BCA Purple Standards 10 800-838-5567 extension 121 (U.S. and Canada) 740-881-5989 (Fax) In-Spec® Nano-Kit 11 001-740-881-5501 (International) Wavelength Standards 12-13 in-spec@gfschemicals.com (e-mail) Stray Light Filters 14 www.in-spec.com Potassium Dichromate 15 www.gfschemicals.com 16 Custom Standards Opening an Account Amco Clear®Turbidity Standards 17 GFS Customer Service Representatives will be glad to help you Application Notes 18-20 open an account. For terms and conditions see Inside Back Troubleshooting Guide 21 Cover. For complete Terms and Conditions, please visit: Analytical & Lab Chemicals 22 http://www.gfschemicals.com/statics/documents/aboutus/ termsandconditions.html Ordering2 3
  3. 3. ContinuousGFS Chemicals has been manufacturing standards forover twenty years for unique customer applications. The In-Spec® product line is constantly growing and currently includes standards to check not just photometric accuracy but also wave- length, stray light, and resolution for a variety of instrumentation.Many of our standards are specific to your industry. Some recent areas of focus have been short pathlength instruments and microplate readers used inSeTTINg The STaNDarD for many pharmaceutical and biotechSpeCTrophoToMeTrIC laboratories.aNalySIS & verIfICaTIoNContinuous improvement at GFSChemicals means:• Development of new standards for the latest instruments. • R&D for new uses and ® applications for the In-Spec® product line.• Investment in a new laboratory Features Benefits to meet higher quality control Non-Toxic No disposal fees standards for the demands of Green Alternative the most stringent industries. Inexpensive Save cost compared to• Certification for ISO 17025 is sealed cuvette alternatives underway. Easy to Use No wasted time with dilutions No special precautions• Experienced technical support to troubleshoot and answer your NIST Traceable Confidence in instrument readings Meets regulatory requirements specific questions. Live Technical Assistance Assistance for method development Help with troubleshooting Customizable Made for your application Multiple pathlengths and wavelengths available Manufactured by GFS In stock and ready to ship Trusted name for quality in the industry4 Improvement 3 5
  4. 4. Selection Gui de In-Spec® How to Use: Choose your wavelength of interest then go across the chart Photometric to find your closest target absorbance value. The top of the Standards column will identify the item number and product name Selection Guide associated with that value. UV Standards Wave- Uv-1 Uv-2 Uv-3 Uv-4 Uv-5 Uv-6 Uv-7 Uv-8 Uv-20 Uv-30 length #8301 #8302 #8303 #8304 #8305 #8306 #8307 #8308 #8309 #8310 250 0.086 0.127 0.177 0.305 0.347 0.447 0.513 0.570 1.135 1.664 254 0.081 0.120 0.165 0.283 0.323 0.416 0.477 0.528 1.054 1.551 In-Spec® UV In-Spec® Visible 260 0.075 0.112 0.152 0.259 0.297 0.382 0.437 0.484 0.967 1.420 Standards Standards 280 0.056 0.870 0.112 0.188 0.220 0.283 0.322 0.355 0.715 1.058 In-Spec® UV Standards are de- The In-Spec® Visible Standards 300 0.039 0.060 0.79 0.131 0.155 0.201 0.230 0.253 0.508 0.754 signed to check the photometric are designed to check the pho- 350 0.011 0.030 0.042 0.072 0.086 0.110 0.127 0.139 0.284 0.420 accuracy of a traditional spectro- tometric accuracy of a traditional photometer from 250nm up to spectrophotometer from 350nm 400 0.007 0.018 0.026 0.045 0.054 0.069 0.080 0.088 0.179 0.266 400nm. up to 850nm. Visible Standards UV Standards - Item Numbers Visible Standards-Item Numbers UV-1: 8301 Vis 0.05: 8311 Wave- vIS-0.5 vIS-0.1 vIS-0.3 vIS-0.5 vIS-0.8 vIS-1 vIS-4 UV-2: 8302 Vis 0.1: 8312 length #8311 #8312 #8322 #8313 #8314 #8315 #8316 UV-3: 8303 Vis 0.3: 8322 400 0.122 0.260 0.724 1.254 1.882 2.436 3.43 UV-4: 8304 Vis 0.5: 8313 450 0.082 0.177 0.500 0.871 1.323 1.780 3.326 UV-5: 8305 Vis 0.8: 8314 500 0.059 0.126 0.360 0.628 0.957 1.298 3.238 UV-6: 8306 Vis 1.0: 8315 550 0.043 0.093 0.270 0.470 0.719 0.975 3.12 UV-7: 8307 Vis 4.0: 8316 600 0.034 0.072 0.212 0.368 0.566 0.767 2.91 UV-8: 8308 UV-20: 8309 650 0.027 0.056 0.169 0.294 0.450 0.611 2.524 UV-30: 8310 700 0.022 0.045 0.137 0.239 0.365 0.497 2.111 Available as sealed quartz cuvettes or bottles (60mL, 125mL, 250mL, 750 0.018 0.037 0.111 0.195 0.299 0.406 1.740 500mL, 1L) 800 0.017 0.030 0.092 0.161 0.247 0.334 1.440 If you need a more precise value please see the customPlease note that the values in the table are representative values only and are to assist in picking out the appropriate standard for products section on page 16.your application. The actual value of the lot you receive may vary. The data provided is representative of a 10mm pathlength cell.6 7
  5. 5. ® ® In-Spec® In-Spec® Amaranth Patent Blue Standard Red Standard Item # 8416 Item #8414 This popular product can be The In-Spec® Amaranth Red standard customized to match a variety of was designed to complement the In- wavelength and concentration levels. Spec® Patent Blue but can be used ex- This reference standard is designed clusively for a variety of other processes. for small volume instruments and When used together, the In-Spec® Patent microplate readers but is equally Blue and Amaranth Red standards cover adept on a traditional spectropho- a wider range of wavelengths that are tometer. Typical applications include associated with applications in pharma- analyses involving DNA, RNA, nu- ceutical and biotech laboratories. This cleic acids, proteins, and enzymes. reference standard is similar to the In- A commonly utilized wavelength for Spec® Patent Blue standard in that it can this reference standard is 260nm. be used on microplate readers, traditional spectrophotometers, and small path- length instruments. Popular wavelengths for this standard are 280nm and 320nm. Sizes Available Sizes Available 60ml 60m l 125ml 125ml_ 250ml 250ml 500ml 500ml 1l 1l Sealed Cuvette Sealed Cuvette8 Standards 9
  6. 6. ® ® In-Spec® BCA Purple Standard In-Spec® Nano-Kit Item # 8587   Item # 8476 The newest addition to the In-Spec® The In-Spec® Nano-Kit was awarded for the product line came about due to innovation in solving the issue of validating short demand to validate colorimeters and pathlength instruments without a toxic material. spectrophotometers used in BCA pro- Prior to the kit’s introduction the only option was tein assays. The wavelength of interest hazardous potassium dichromate solutions. The for this application is 562nm. This In-Spec® Nano-Kit consists of our popular Patent reference standard is equally customi- Blue and Amaranth Red reference standards with zable as its Patent Blue and Amaranth standard data at 260nm, 280nm and 320nm. Red counterparts. The data is certified with a 1mm pathlength cu- vette eliminating the conversion from the 10mm Sizes Available pathlength reading which is typical with many 60ml other standards. 125ml The reference standards are guaranteed for 1 50ml year from the date of shipment unlike many com- 500ml peting materials that are a one-time use product. 1l Sealed Cuvette10 Standards Nano-Kit 11
  7. 7. Wavelength Accuracy Wavelength Accuracy Standards The accuracy of your instrument’s wavelength scale is crucial when obtaining absorbance readings on your sample. A slight change of less than 1nm can cause an instrument’s read- ings to shift leading to incorrect data. This puts the laboratory at risk of passing a product that should have failed quality control. GFS Chemicals offers 3 different wave- length reference filters that are used to validate the wavelength scale. These materials will add confidence to your quality control process. Listed below are the wavelength refer- Didymium Oxide Wavelength Standard Item # 8378 ence filters available with associated Peaks at: 298, 329, 355, 445, 469, 482, 512, 522, 576, 732, 740, 795, 802, 866nm peaks. These materials are available in sealed quartz cuvettes certified for 4 years or in HDPE bottles with a 1 year shelf life. Recertification is available for the sealed quartz cuvettes. Holmium Oxide Wavelength Standard Item # 8340 Samarium Oxide Wavelength Standard Item # 8379 Peaks at: 241, 250, 278, 287, 334, 345, 361, 386, 416, 451, 468, 485, 537, 641 nm Peaks at: 236, 267, 280, 290, 305, 318, 332, 345, 401, 416, 442, 464, 479 nm12 13
  8. 8. Stray Light Filters Filters Stray radiant light is energy reaching the detector that is not originating from the Potassium Dichromate Traditionally, potassium dichromate was used to check the photometric accuracy of an instrument. GFS Chemicals Inc. offers 5 different concentration levels. Using 3 or more of these values will allow you to check the linearity of your instru- instrument light source. This can come from a variety of sources and impacts ment. These standards come sealed in 10mm quartz cuvettes the readings output by the instrument. Stray light can be a problem at any wave- or in bottles and are designed for use in the UV spectrum. The length but is more prevalent in the UV range. sealed standards are certified for 1 year from shipment and recertification service is available. Custom concentrations are The use of a stray light filter can verify that an external light source is not effecting available. Please see the custom section on page 16. the results. There are five commonly used materials to check the UV range of an instrument. Each standard has a ‘cut-off’ wavelength and eliminates light trans- Catalog Numbers mission below that level. Listed below are the standards offered and their associ- 20mg/L Item #8350 ated ‘cut-off’ wavelength. These are available in sealed quartz cuvettes certified for 40mg/L Item #8368 2 years or sealed HDPE bottles with a 1 year shelf life. Recertification is available. 60mg/L ltem #8369 80mg/L Item #8348 100mg/L Item #8372 Background Solution Item # 8349 Potassium Iodide: 260nm Item #: 8380 Lithium Carbonate: 227nm Item #: 8382 Potassium Chloride: 200nm Item #: 8384 Sodium Iodide: 260nm Item #: 8381 Sodium Chloride: 205nm Item #: 838314 15
  9. 9. ®You may have a unique application Custom Standardsthat requires verification of certainabsorbance values or wavelengths AMCO Clear®that aren’t listed for our stock products. Turbidity StandardsOur experienced staff has the ability Amco Clear® is an EPA approved primary standard for cali-to work with you to develop a refer- bration of turbidity sensors. It is available in a wide range ofence standard that will validate your values. It has a guaranteed one year shelf life. The convenientinstrument for your application. submicron polymer suspensions are non-toxic primary calibration standards approved by the US EPA for drinkingPlease call technical support for water analysis for turbidity.assistance. Amco Clear® is “The Clear Choice in Turbidity.”In order to facilitate custom standarddevelopment there are several keypieces of data that are required.With this information, technicalsupport will be able to quickly assessyour needs.• Wavelength(s) of interest• Pathlength• Instrumentation make and model• Desired or sample absorbance rangeOur problem-solving lab team isavailable Monday through Fridayfrom 7:30 am to 4:00 pm EST. AMCO Clear® Advantages: • Safe, non-toxic and disposable • Stable—does not settle out of suspension • Requires no mixing or inverting • Easy-to-Use/ No dilutions or preparations • Accurate to 1% lot-to-lot Custom • Guaranteed one year shelf life from date of shipment • Available in a wide range of values • NIST Traceable • Custom Standards Available16 17
  10. 10. Application Notes Specific Uv absorbance (SUva) for Total organic Carbon (ToC) analysis Standards for validation of Microplate Readers & Short Pathlength Instruments Specific UV Absorbance (SUVA) is an analysis of drinking water that With growing demand to for smaller sample analysis, microplate readers are becoming uses UV absorbance at 254nm to identify dissolved organic carbon more common place in the biotech/pharmaceutical industry. (DOC) levels. UV absorbance is easily determined using a spectropho- These instruments are typically used in performing various tests in relation to the analysis tometer. Previous studies established a relationship between SUVA of DNA/ RNA, protein and enzymes. Many of the options that are used to verify the accu- and the levels of humic substances that are removed during enhanced racy of these instruments are either expensive or limited in the scope that they can check. coagulation and/or enhanced softening. Utilizing this relationship allows for a cheap and easy method to determine whether enhanced coagula- GFS Chemicals has developed standards to validate the accuracy of instruments with equal tion or softening is required based off of the exemption allowed in Stage to or less than 1mm pathlengths. The most popular application at this time is for users 1 D/DBPR and Stage 2 D/DBPR. Removing the extra disinfection from checking at 260 and 280nm. the process can save a water district money when it comes to TOC removal. This type of validation is where the In-Spec® Color standards work perfectly. The color standards are designed to work with the built in software programs that manipulate the Under Stage 1 D/DBPR, Standards Method 5910B was the only EPA data obtained in the plate and displays it as optical density. The color standards have many approved method for SUVA determination. Stage 2 D/DBPR approved useful wavelengths, as exhibited on page 8 and 9. These can also be made in a wide range EPA Method 415.3 (Revision 1.1, 2005) for TOC, DOC and SUVA of concentrations to better match the values you see in your daily testing. determination. This one method not only combines all three tests, but also provides increased quality control procedures to ensure testing ac- The color standards are available in a wide range of sizes and have a 1 year shelf life so curacy, including calibration verification procedures for all instruments you can keep them on the shelf and ready to test your instrument when the need arises. All utilized in the method. you need to do is pipet an aliquot into the wells on your plate and run them on the reader. Once the data is obtained you then compare that to the values on the certificate of analysis According to the EPA method, the verification of the spectrophotom- to verify that the instrument is performing as it should. eter can be done with a commercially prepared check standard. This GFS Chemicals Inc. also offers Holmium Oxide solution in individual bottles so that you method specifically mentions the In-Spec® photometric standards, may verify the wavelength scale on your scanning microplate reader. This provides a much manufactured by GFS Chemicals, Inc, which were utilized by the cheaper alternative to sealed plates with Holmium Oxide or a sealed quartz cuvette. EPA to validate their spectrophotometer when developing 415.3. The In-Spec® photometric standards are NIST traceable, non-toxic, have a By utilizing both the color standards and the Holmium Oxide solution you will be able to say one-year shelf life and require no preparation to use. with confidence that the data you obtained on your microplate reader is accurate based off NIST-traceable standards. Data provided at 254nm for In-Spec UV Standards 1 through 30.18 19
  11. 11. amaranth red and its use in the Wine Industry Notes The In-Spec® Amaranth Red standard is ideal for use in the TroubleShooting Guide If your reference standards are not reading within the tolerance levels stated on the certificate of analysis utilize the following guide prior to calling for techni- cal support. Having the answers to the following questions will help expedite the troubleshooting process. wine industry for some common testing that is performed on 1) When was the last time the lamp was changed? colorimeters or spectrophotometers. Wavelengths commonly tested in wine samples for color intensity, hue, and total phe- a. GFS replaces the lamps in our instrument every 6 months, but with normal nolic concentration analysis are 520nm, 420nm, & 280 nm. use, changing the lamps every 2 years should be sufficient. b. Many older spectrophotometers do not have diagnostic capabilities to tell the The color standards can be used to validate your instrument user when lamp energy is depleted or how many hours they have been in use. so that you know that it is performing as it should or it can be used as an inter-lab check so that you know your instrumen- 2) Are your cuvettes/cells clean, scratch-free and suitable for your range of use? tation in one lab is matching that in another lab. Over time an a. All data provided has been obtained in a 10mm FUV quartz cuvette unless instruments light source may dim and the values you obtain stated in the certificate of analysis. will start to drift. b. Do not allow SDVB suspensions to dry on or inside your cells as it is difficult to remove. Knowing when this is occurring allows you to perform the c. Disposable plastic cuvettes are generally not designed for the UV range unless necessary maintenance before the issue impacts the quality specified on the package. of the wine that you are producing. Multiple concentrations are available so that you may check a broad range of absorbance values. 3) Was the supplied background solution* used to provide a baseline that reads 0.0? a. GFS has found that the “baseline” function alone may not provide a 0.000 ab- sorbance when the background solution is scanned later. It may take a combina- tion of “Auto zero” and “Baseline” over the range of interest. b. See Certificate of Analysis for appropriate lot number of background solution. 4) How recently has your instrument had a full calibration that included wavelength scale certification and stray light detection? a. GFS has this performed twice yearly by the manufacturer b. GFS provides NIST traceable wavelength and stray light check standards as well. 5) What spectral bandwidth are you using on your instrument? a. All provided data is for a 1nm bandwidth unless otherwise specified. b. Higher bandwidths often provide lower resolution and affect the values ob- Guide tained. *Supplied with In-Spec UV and Visible standards20 21
  12. 12. GFS Chemicals - Innovative Chemical Technology of the Society of Chemical Manufacturers & Affili- for Innovative Applications ates (SOCMA) and the Ohio Chemistry Technology Council, GFS is committed to conducting our Analytical and Laboratory Chemists and technicians can connect to a single business with integrity and foresight. In addition, Chemicals company with manufacturing resources in all key areas of chemistry. Customers rely on us for our GFS is committed to full implementation of the SOCMA® ChemStewards® environmental, health, problem solving ability. Our proven technologies, safety and security management system. GFS manufactures over 6,000 organic and inorganic technical expertise, engineering excellence and attention to detail allow us to work hand-in-hand Why Choose GFS Chemicals? analytical reagent chemicals and specialties in a lean with our customers every step of the way – from manufacturing operation. We have most chemicals startup to commercialization. GFS technology and • 80+ years of chemical manufacturing and chem- products have kept us steps ahead of the indus- istry experience supporting process and needed for laboratory, bench, analytical, quality control, try’s ever- changing chemical needs. commercial applications materials characterization, scientific or research work. • Knowledgeable chemists and operators who GFS Chemicals began when Professor G. Freder- have a complete understanding of the ick Smith started producing perchloric acid in manufacturing process Our ultra-high purity double-distilled Veritas® and ACS a small factory in Columbus, Ohio. Since then, • Scale-up flexibility from small sample sizes to GFS has grown to become a leading producer of drums to metric tons, to ensure consistent quality Superior™ brand acids and Watermark® brand reagents specialty chemicals with performance-proven tech- from R&D to pilot to final production are used throughout the world. Our AMCO Clear® nologies for laboratory, analytical, scientific, • International expertise shipping products to 85+ Water Analysis Division manufactures the highest water research and development, pilot scale production, countries means your products will arrive on time, and manufacturing applications. meeting all regulatory compliance regulations calibration standards available for water treatment plants • Technical support and resources every step of around the globe. Project Customization the way • Dedicated, responsive customer support Our production environment gives us the flexibility personnel and points of contact • Watermark® pyridine-free • Silver Compounds to offer you innovative solutions for all of your • Competitive pricing and reliable production Karl fischer reagents • Reagents for Water chemical needs. GFS research and development lead times can support you with the chemicals that your • U.S.-based quality manufacturing to ensure • Veritas® hplC Solvents & Wastewater labs application requires. Using our proven technology material availability, consistency and delivery • HPLC/GC/UV-Vis Solvents • Organic Halogen Reagent – to customize your product, we create customer- • Experienced staff who understand the complexi- • Reagent Grade Solvents OHR/Halogen Level Field specific item numbers and SKUs to streamline ties and dependencies of process chemistry • Spectroscopy Products TestKits and safeguard your process. Our formalized and repeatable ISO manufacturing ensures that you get Associations and Affiliations • Primary Standard Salts • Polyethylene Glycol the same exact material every time. We package GFS Chemicals has been actively involved in the • Normality Solutions (small quantities) according to your requirements in small packages scientific community for generations. Member- through drums. ships include: • Colors and Indicators • Conductivity Standards and If your application requires more stringent condi- • American Chemical Society ph Buffers tions, we have experience with Good Manufac- • Society of Chemical Manufacturers and • AA and ICP Standard Solutions turing Practice (GMP) processes. We are a key Associates (SOCMA) supplier of critical materials for numerous Fortune • Tier II ChemSteward® Certified for Environmental • Anion Standard Solutions 100, GMP manufacturers. We understand the Health and Safety • Standard Solutions for needs of our GMP customers – including pharma- • American Water Works Association and Stan- Trace Metal analysis ceutical manufacturers. dard Methods for the Examination of Water and Wastewater • ACS Reagents Unmatched Product Quality • Water Environment Federation • ASTM Type I Deionized water • Ohio Water Environment Association GFS is an ISO 9001-2008 certified company. Our • ASTM International - Committees D19, E13, quality control team guarantees product consis- E15 and E27 tency from lot-to-lot. We guarantee our products • American Council of Independent Laboratories- to meet the quality standards that we publish, and Associate member ship a Certificate of Analysis and a Material Safety • Ohio Manufacturers Association Data Sheet (MSDS) with every order. • Chemical and Specialties Management Council • Ohio Chemistry Technology Council Environmental and Community Stewardship • U.S. Chamber of Commerce • Ohio Chamber of Commerce GFS was founded on a business philosophy of • National Federation of Independent Businesses community stewardship and respect for our envi- • National Association of Manufacturers ronmental resources. As a long-standing member • Tech Columbus22 23
  13. 13. Team GFS Are you sure your instrumentation is properly reading your samples? Do your standard operating procedures require a vali- dation standard? Over time small changes in your instrumenta- tion can affect the data you obtain. Our reference standards are a high quality, in-expensive way to add confidence to your measurements. GFS Chemicals Inc offers a full line of spectrophotometer validation standards. • Wavelength reference standards • Photometric reference standards • Stray Light reference standards Contact information: e-mail in-spec@gfschemicals.com 800-838-5567 extension 121 for technical assistance, pricing and availability (M-F 7:30 am to 4:00 pm Eastern Standard Time) 614-824-3299 740-881-5989 FAXCHEMICALS ® Visit www.in-spec.com for technical information or Order online at www.gfschemicals.com