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In your practical, you were given pOTC- DNA restriction digested using three different enzyme
mixes containing KpnI alone, NdeI and NotI and another containing SphI and NheI. The identity
of each mix was unknown as they were labelled as enzyme mix A, B and C.
Enzyme A mix: Ndel & Notl / Sphl & Nhel / Kpnl (Choose 1 of the answers)
Enzyme B mix: Ndel & Notl / Sphl & Nhel / Kpnl (Choose 1 of the answers)
Enzyme C mix: Ndel & Notl / Sphl & Nhel / Kpnl (Choose 1 of the answers)
DNA Ladder

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In your practical, you were given pOTC- DNA restriction digested usi.pdf

  • 1. In your practical, you were given pOTC- DNA restriction digested using three different enzyme mixes containing KpnI alone, NdeI and NotI and another containing SphI and NheI. The identity of each mix was unknown as they were labelled as enzyme mix A, B and C. Enzyme A mix: Ndel & Notl / Sphl & Nhel / Kpnl (Choose 1 of the answers) Enzyme B mix: Ndel & Notl / Sphl & Nhel / Kpnl (Choose 1 of the answers) Enzyme C mix: Ndel & Notl / Sphl & Nhel / Kpnl (Choose 1 of the answers) DNA Ladder