This document summarizes the TruSeq small RNA library preparation workflow. It involves ligation of 5' and 3' adapters to small RNA fragments. The 5' adapter contains a sample index sequence and the 3' adapter forms a stem loop structure to prevent self ligation and ensure ligation only to small RNA fragments.

1. Esquema
TruSeq small RNA
5' R1====================> 3'
_RA5______________________ _RA3_________________
_RP1______________________________________________
AATGATACGGCGACCACCGAGATCTACACGTTCAGAGTTCTACAGTCCGACGATC INSERTO TGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC[NNNNNNN]TCTCGTATGCCGTCTTCTGCTTG
TTACTATGCCGCTGGTGGCTCTAGATGTGCAAGTCTCAAGATGTCAGGCTGCTAG ACCTTAAGAGCCCACGGTTCCTTGAGGTCAGTG[NNNNNNN]AGAGCATACGGCAGAAGACGAAC
___________________________________________________________01IPR_
ACCTTAAGAGCCCACGGTTCCTTGAGGTCAGTG ATCGAAT AGAGCATACGGCAGAAGACGAAC
___________PTS_
TTGCACCACCTTAAG 5' 5'
< |||||
CCCGTGG 3'
_______
* STP oligo forms a stem loop and one end of the stem loop anneals to the 3' adapter oligo and inhibits further ligation.