How to Read & Save A Run In Quant Studio 5

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I aim to explain the process of recording the run once the process of RT-PCR has been successfully completed. According to ICMR, the Ct (Cycle Threshold ) value is capped at 35 for a sample to be given as positive. A Ct value more than 35 would be given Negative. Link to the Video - https://youtu.be/h0ovoRi8N-g

Education

HOW TO READ & SAVE A RUN
IN QUANT STUDIO 5
MAYUR D. CHAUHAN
M.Sc. Biotechnology

Click on the following link for
a detailed explanation video
https://youtu.be/h0ovoRi8N-g

Tuberculosis (TB) is one of the most serious diseases which affect millions of people annually. It is caused by Mycobacterium tuberculosis (MTB), specifically causing tuberculosis in humans. Tuberculosis infection spreads mainly through inhalation of the aerosolized bacteria present in the form of droplet nuclei. These infective droplets are coughed or sneezed into the environment by the patients suffering from pulmonary tuberculosis. The bacteria multiply within the alveolar macrophages and might spread to other parts of the body. Thus tuberculosis should be treated effectively by providing the patients with proper medication or else the strain develops resistance against the drugs. MDR - TB has become prevalent mainly due to late diagnosis and inadequate treatment regimens. Tuberculosis is detected by culturing the specimens on the Lowenstein and Jensen (LJ) medium. MTB, being a slow growing strain, takes about 6-8 weeks of time to show demonstrable colonies on the medium. Biochemical testing for speciation was time consuming and gives varied results for species which were genetically similar. Thus there was a need for rapid detection of tuberculosis infection which would help in adequate treatment of the patients without allowing the progression of the disease. Liquid culture was introduced in the year 1980’s which reduced the turnaround time to 2-3 weeks. Drug sensitivity testing by the conventional solid or liquid methods took an additional 4-6 weeks and 2 weeks respectively. Many molecular methodologies have been adopted for rapid detection of tuberculosis. Genotype MTBDRplus assay (Hain Life science) is a Line Probe Assay which amplifies the mycobacterial DNA and detects the sensitivity or resistivity of the strain against first - line anti-tubercular drugs (Rifampicin and Isoniazid). GeneXpert (Cepheid) is an advanced technology in detecting tuberculosis and it’s susceptibility to Rifampicin only. It is a semi quantitative Nested PCR and is based on the principle of Cartridge Based Nucleic Acid Amplification Testing (CB - NAAT). It gives the result within 2 hours. Thus we discuss the exact procedure of these techniques which can detect tuberculosis rapidly and can help to treat the patients adequately.

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