The document summarizes experiments studying the growth of Methanosarcina acetivorans with carbon monoxide as the substrate. Several hypothesized pathways and experimental methods are mentioned, including changing substrates, scrubbing CO, pressurizing bottles, and creating gene knockouts. The results were initially negative with nothing observed, but the methods were adjusted and the experiments are ongoing.

3. growth of Methanosarcina acetivorans with carbon monoxide as the substrate Heather Jordan Department of Biochemistry and Molecular Biology The Pennsylvania State University University Park, Pennsylvania 16802

4. A Method to the Madness…

5. Hypothesized Pathway 1

26. Hypothesized Pathway 2

46. Withdrawing CO for Pressurization

47. Pressurizing the Serum Bottle

48. Greatest Surface Area

49. Results

53. To be continued…

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