The document describes experiments conducted by a team to identify unknown bacteria samples from a belly button (U/U) and leaf (known unknown). They determined via gram staining that the belly button bacteria were gram positive cocci arranged in clusters. For the leaf bacteria, they determined it was gram positive rods arranged randomly. They narrowed down the potential identities of the bacteria based on morphological characteristics and growth on different media types. Suspect identifications for the belly button included S. epidermidis and S. aureus while suspects for the leaf included B. cereus and B. subtilis. Tests on various media measured growth and acid/gas production.

1. Bacteria From Natural Habitats
JR’s Belly Button & Dr. Mikel’s Leaf
Team 1D Members Include:
Andrea Taylor
Nasrin Saber
Gina Blanchet-Parker
Jose Rey Manaois
General Microbiology 260 / Lab D

2. Our Unknown Bacteria
1. Unknown Unknown (U/U): Belly button
bacteria from JR Manaois
2. Known unknown: Leaf bacteria provided by
Dr. Mikels

3. Our Investigation for Our
“Unknown / Unknown”
After Gram staining the culture of the Belly Button
Bacteria and it’s colonies, we were able to determine the
following:
• Gram + (Purple Stain)
• Shape: Cocci
• Arrangement: Clusters
OUR PHOTO
• Gram + (Purple Stain)
• Shape: Cocci Samples of other Gram stained S.
epidermis bacteria
• Arrangement: Clusters

4. Our Investigation for Our
“Unknown / Unknown”
After Gram staining the culture of the Belly Button
Bacteria and it’s colonies, we were able to determine the
following:
• Gram + (Purple Stain)
• Shape: Cocci
• Arrangement: Clusters
OUR PHOTO
• Gram + (Purple Stain)
• Shape: Cocci
• Arrangement: Clusters Samples of Gram stained S. cereus
bacteria

5. Narrowing down the
“Unknown Unknown”
(Belly Button)
After Gram staining the culture of the Belly Button
Bacteria and it’s colonies, we were able to eliminate
all bacteria with the following attributes
• All G- bacteria
• All bacteria shapes other
than coccus.
• All bacteria not arranged in
clusters.

6. Narrowing down the
“Unknown Unknown”
(Leaf)
After Gram staining the culture of the Leaf Bacteria
and it’s colonies, we were able to eliminate all
bacteria with the following attributes
• All G- bacteria
• All bacteria shapes other
than rods.
• All bacteria not arranged at
random.

7. Which Bacteria Should Still
Be Considered for the
“Unknown / Unknown”for the Leaf?
Gram + Bacteria Growth
1. B. cereus
3
• Info S. epidermidis
Grow th Seen S. aureus
2 2 2 2
2. B. subtilis
0 = None S. agalactiae
1 = Slight
2 = Moderate
M. rosea
3 - Abundant
• Info
B. subtilis
1
B. cereus
Unknown/Unknown (Belly
Button)
0 Known/Unknown (Leaf)
0
PEA BA MSA - TSA
SALT
Type of Media Used

8. Which Bacteria Should Still Be
Considered for the “Unknown / Belly
Button?
Gram + Bacteria Growth
1. S. epidermidis
• Info Natural Flora found on Skin blah, blah, blah 3
S. epidermidis
Grow th Seen S. aureus
2. S. aureus
2 2 2 2
0 = None S. agalactiae
1 = Slight
2 = Moderate
M. rosea
3 - Abundant
• Info 1
B. subtilis
B. cereus
Unknown/Unknown (Belly
Button)
0 Known/Unknown (Leaf)
0
PEA BA MSA - TSA
SALT
Type of Media Used

9. A little something about our suspects
for the Leaf
1. B. cereus
• Info
2. B. subtilis
• Info

10. A little something about our suspects
for Belly Button
1. S. epidermis
• Info
2. S. aureaus
• Info

11. Growth Tests
G- Bacteria on Variety of Media
3.5
3
3
P. vulgaris
2.5 K. pneumoniae
2 E. coli
Growth 2
S. marcescens
0 = None
1 = Slight E. aerogenes
1.5
2 = Moderate P. aeruginosa
3 - Abundant 1
1 Belly Button (u/u)
Leaf (k/u)
0.5
0
PEA EMB SSA TSA
PEA = Phenylothanol BA = Blood Ager
EMB = Eosin Methylene Blue (EMB)
SSA = Salmonella-Shigella Agar
TSA = Trypticase Soy Agar

12. Growth Tests
G+ Bacteria on Variety of Media
Production of Carboxylic Acid & Gas Phenol Red Medias
1
S. epidermidis
S. aureus
S. agalactiae
M. rosea
B. subtilis
B. cereus
Unknown/Unknown (Belly Button)
Known/Unknown (Leaf)
0
PRG - ACID PRG - GAS PRA - ACID PRA - GAS PRL - ACID PRL - GAS PRS- ACID PRS - GAS PRM - ACID PRM - GAS
Media Used
Acid Key Gas Key
Phenol Red Glucose (PRG)
0 = No 0 = No Gas
Phenol Red Lactose (PRL)
1 = Yes 1 = Yes, Gas
Phenol Red Maltose (PRM)
Phenol Red Sucrose (PRS)
Phenol Red Arabinose (PRA)

13. Growth Tests
G- Bacteria on Variety of Media
Production of Carboxylic Acid & Gas Phenol Red Medias
1
P. vulgaris
K. pneumoniae
E. coli
S. marcescens
E. aerogenes
P. aeruginosa
0
PRG - ACID PRG - GAS PRA - ACID PRA - GAS PRL - ACID PRL - GAS PRS- ACID PRS - GAS PRM - ACID PRM - GAS
Media Used
Acid Key Gas Key
Phenol Red Glucose (PRG)
0 = No 0 = No Gas
Phenol Red Lactose (PRL)
1 = Yes 1 = Yes, Gas
Phenol Red Maltose (PRM)
Phenol Red Sucrose (PRS)
Phenol Red Arabinose (PRA)

14. Gram – Bacteria
on a Variety of Media
3
S . e p id e rm id is
S . au re u s
Grow th See n
2 2 2 2
0 = None S . agal tiae
ac
1 = Slight
2 = Mode rate
M . ro s e a
3 - Abundant
B . s u b tilis
1 1
B . c e re u s
U n k n o wn /U n k n o wn (B e l y B u tto n )
l
K n o wn /U n k n o wn (Le af)
0
PEA BA MSA - MSA - TSA
SALT FERM
Type of Me dia

15. PARAGRAPHS

16. PARAGRAPHS