We report the 11-year follow-up of a premenopausal woman with osteogenesisimperfecta (OI) who
was treated with alendronate. A 41-year-old Japanese premenopausal woman with OI type I who had
frequently experienced painful fragility fractures consulted our clinic because of chronic back pain associated
with spinal osteoporosis. She had undergone heart surgery (aortic valve replacement) because of aortic
regurgitation 5 years before her first consultation with our clinic. After surgery, she began taking warfarin (3
mg/day), and this treatment was continued during our follow-up period. She was treated with alendronate (5
mg/day or 35 mg/week) for 11 years. The patient’s urinary cross-linked N-terminal telopeptides of type I
collagen and serum alkaline phosphatase levels decreased, while the bone mineral density of her lumbar
spine (L2–L4) increased, as measured using dual energy X-ray absorptiometry. The serum calcium and
phosphorus levels stayed within the normal ranges. Three non-vertebral fractures occurred at the hip, ankle,
and ring finger during the 11-year treatment period, but no adverse effects were observed. Thus, the present
case report showed the long-term outcome and safety of alendronate treatment in a premenopausal woman
with OI type I.
Increase in Bone Mass Density and Overall Bone Health Following High-Impact o...CrimsonPublishers-SBB
Vitamin D3 and calcium are the main bone health supplements required for quality of life. The present study aimed to find out the role of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters in vitro using MG-63 cells. The parameters of bone health tested are Alkaline Phosphatise Enzyme (ALP) activity, collagen levels, and bone mineralization. The Test Items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Krista Joanne Callas and samples were defined as the Bio-field Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay exhibited increased cell viability range from 72% to 122% with safe and nontoxic profile among test samples on the MG-63 cell line. was significantly increased by 129.6%, 392%, and 255.7%in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively at 100μg/mL as compared with the untreated group.
We report the 11-year follow-up of a premenopausal woman with osteogenesisimperfecta (OI) who
was treated with alendronate. A 41-year-old Japanese premenopausal woman with OI type I who had
frequently experienced painful fragility fractures consulted our clinic because of chronic back pain associated
with spinal osteoporosis. She had undergone heart surgery (aortic valve replacement) because of aortic
regurgitation 5 years before her first consultation with our clinic. After surgery, she began taking warfarin (3
mg/day), and this treatment was continued during our follow-up period. She was treated with alendronate (5
mg/day or 35 mg/week) for 11 years. The patient’s urinary cross-linked N-terminal telopeptides of type I
collagen and serum alkaline phosphatase levels decreased, while the bone mineral density of her lumbar
spine (L2–L4) increased, as measured using dual energy X-ray absorptiometry. The serum calcium and
phosphorus levels stayed within the normal ranges. Three non-vertebral fractures occurred at the hip, ankle,
and ring finger during the 11-year treatment period, but no adverse effects were observed. Thus, the present
case report showed the long-term outcome and safety of alendronate treatment in a premenopausal woman
with OI type I.
Increase in Bone Mass Density and Overall Bone Health Following High-Impact o...CrimsonPublishers-SBB
Vitamin D3 and calcium are the main bone health supplements required for quality of life. The present study aimed to find out the role of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters in vitro using MG-63 cells. The parameters of bone health tested are Alkaline Phosphatise Enzyme (ALP) activity, collagen levels, and bone mineralization. The Test Items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Krista Joanne Callas and samples were defined as the Bio-field Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay exhibited increased cell viability range from 72% to 122% with safe and nontoxic profile among test samples on the MG-63 cell line. was significantly increased by 129.6%, 392%, and 255.7%in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively at 100μg/mL as compared with the untreated group.
Vicki Harber
LA GIOVANE ATLETA
Il ciclo mestruale, punto di riferimento per un sviluppo sano della giovane atleta
Nel processo di sviluppo delle giovani atlete è necessario che siano integrati il monitoraggio continuo del menarca e il controllo del loro stato mestruale. Promuovere lo sviluppo di una giovane atleta e supervisionarne l’allenamento è impegnativo e complesso. Se dispongono di una conoscenza maggiore della funzione mestruale le giovani atlete e le loro famiglie hanno strumenti migliori per rispondere alle esigenze dell’allenamento e delle gare. Lo stato mestruale rappresenta un indicatore globale della salute e del benessere che fornisce informazioni che riguardano l’energia, il rischio di lesioni scheletriche e muscolari, l’apporto alimentare, il profilo metabolico e ormonale, il recupero e altri elementi, importanti per la prestazione. Inoltre, con l’uso crescente dei contraccettivi orali da parte delle giovani atlete che non hanno raggiunto la loro maturità scheletrica, allenatori, allenatrici e genitori debbono essere informati dei risultati recenti che riguardano la salute delle ossa.
LA FORZA NELLA DONNA UN PROGRESSO INARRESTABILE
Renato Manno
Caratteristiche, specificità di genere, effetto dell’età e dei tipi di allenamento
SDS SCUOLA DELLO SPORT 105
http://www.calzetti-mariucci.it/shop/prodotti/sds-scuola-dello-sport-n-105
People may suffer from hip problems, their age notwithstanding. Thus, hip replacement surgery was developed.As reported by the Bloomberg, US Democrats are pushing for policy blocking the distribution of medical devices that have safety problems. They talked about untoward incidents brought about by medical products of Johnson & Johnson. Design problems with metal-on-metal hip implants may have caused the metal components of the DePuy device to rub against each other and shed microscopic metal particles into the body. Read more here about Depuy Settlements at this website: www.depuysettlements.com
Prof. Jon Tobias's presentation from Osteoporosis 2016: What are the properties of the perfect therapy?
Find out more at: https://nos.org.uk/conference
Vicki Harber
LA GIOVANE ATLETA
Il ciclo mestruale, punto di riferimento per un sviluppo sano della giovane atleta
Nel processo di sviluppo delle giovani atlete è necessario che siano integrati il monitoraggio continuo del menarca e il controllo del loro stato mestruale. Promuovere lo sviluppo di una giovane atleta e supervisionarne l’allenamento è impegnativo e complesso. Se dispongono di una conoscenza maggiore della funzione mestruale le giovani atlete e le loro famiglie hanno strumenti migliori per rispondere alle esigenze dell’allenamento e delle gare. Lo stato mestruale rappresenta un indicatore globale della salute e del benessere che fornisce informazioni che riguardano l’energia, il rischio di lesioni scheletriche e muscolari, l’apporto alimentare, il profilo metabolico e ormonale, il recupero e altri elementi, importanti per la prestazione. Inoltre, con l’uso crescente dei contraccettivi orali da parte delle giovani atlete che non hanno raggiunto la loro maturità scheletrica, allenatori, allenatrici e genitori debbono essere informati dei risultati recenti che riguardano la salute delle ossa.
LA FORZA NELLA DONNA UN PROGRESSO INARRESTABILE
Renato Manno
Caratteristiche, specificità di genere, effetto dell’età e dei tipi di allenamento
SDS SCUOLA DELLO SPORT 105
http://www.calzetti-mariucci.it/shop/prodotti/sds-scuola-dello-sport-n-105
People may suffer from hip problems, their age notwithstanding. Thus, hip replacement surgery was developed.As reported by the Bloomberg, US Democrats are pushing for policy blocking the distribution of medical devices that have safety problems. They talked about untoward incidents brought about by medical products of Johnson & Johnson. Design problems with metal-on-metal hip implants may have caused the metal components of the DePuy device to rub against each other and shed microscopic metal particles into the body. Read more here about Depuy Settlements at this website: www.depuysettlements.com
Prof. Jon Tobias's presentation from Osteoporosis 2016: What are the properties of the perfect therapy?
Find out more at: https://nos.org.uk/conference
These slides use concepts from my (Jeff Funk) course entitled Biz Models for Hi-Tech Products to analyze the business model for 23andMe’s personal genomics service. 23andMe provides personal health care analysis and ancestry information to individuals that are based on a partial analysis of an individual’s DNA data. Driven by the falling cost of DNA sequencing, 23 and Me provides this information for $99 and a small amount of saliva. While this business model may succeed particularly if the FDA eventually approves the health care portion of the service, we recommend that 23andMe develop a new business model. It should offer the service for free to individuals, particularly those who frequent gyms and nutrition stores and sell information about potential athletes to sports teams.
The history of sports probably extends as far back as the existence of people as active beings. The history of sports informs a great deal about social changes and about the nature of sport itself.
In the current era, as technology changes at an exponential rate, the social impacts are varied and deep rooted. This implies a great deal of change in sports too.
As we move into the era of robotics, machines, transhumans, cyborgs, prosthetics and exoskeletons, the future of sports seems even more disrupted. And it raises questions at different levels.
On the ground level, how will sports and sports-viewing experiences evolve? On the next level, how will the sportsperson of the future look like? And on a much higher level, we raise questions on ethics in sports, sportsmanship, and team spirit.
The Incidence of Traumatic Posterior and Combined Labral Tears in Patients Un...Lennard Funk
Presentation at ISAKOS, 2019
There were 442 primary arthroscopic labral repair procedures performed over the three-year period. The total cohort had a mean age of 25.91±9.09 years (range, 14-67 years) and consisted of 89.6% males. There was no significant difference in mean age or gender between the isolated anterior, posterior or combined groups (p=0.383 and p=0.541, respectively).
• Of the 442 patients who underwent a shoulder labral repair, isolated anterior labral pathology occurred in 52.9% (n=234), with posterior and combined labral tears accounting for 16.3% (n=72) and 30.8%, respectively (n=136) (Table 3).
• Patients were stratified as either sporting or non-sporting; 74.9% of patients were categorised as sporting (n=331) and had a mean age of 24.91±5.69 years, which was significantly lower than the mean age of 35.40±11.94 years in the non-sporting population (p<0.001). In the non-sporting population 68.5% (n=76) of patients had isolated anterior labral tears with 12.6% (n=14) posterior and 18.9% (n=21) combined. In the sporting population isolated anterior labral tears accounted for 47.7% (n=158), posterior 17.5% (n=58) and combined labral tears 34.7% (n=115). The sporting population had a significantly greater proportion of posterior and combined labral tears with the non-sporting population a significantly greater proportion of anterior labral tears (p=0.013).
• Rugby players had the greatest incidence of shoulder instability within the sporting cohort accounting for 231 cases. Of the 231 cases, 47.2% were isolated anterior labral tears, 12.6% isolated posterior and 40.3% combined lesions.
Posterior and combined shoulder labral tears are more prevalent than previously reported in the civilian population. The rates are higher in young, sporting populations and especially in contact sports such as rugby.
Can anterior cruciate ligament injuries be mitigated?Ella Ward
Overall, despite much scientific research, the incidence and prevalence of ACL injuries still remains high. I do not believe ACL injuries can be completely mitigated as they are so multi-factorial and involve both contact and non-contact situations. And although preventative programs have been found to reduce the incidence of ACL injuries by up to 90% in some cases, I still believe a lot more research needs to be conducted in regards to how much training stimulus is required to produce a protective effect. I understand that many coaches may be sceptical in regards to employing a preventative program, but the research indicates even something as simple as plyometric training can help train neuromuscular control and improve lower leg strength. Overall, I believe something is better than nothing, and if more often than not intervention programs are producing positive outcomes, the coaches really have nothing to lose.
The key is to achieve widespread awareness and widespread use; so spread this presentation and message so we can achieve this and help reduce the debilitating consequences this injury has on so many young athletes.
Some Mechanisms of the Noncontact Anterior Cruciate Ligament (ACL) Injury among Male Sport Activities by
Kasbparast Mehdi in Examines in Physical Medicine & Rehabilitation
Crimson Publishers-Fragility Hip Fracture and Sarcopenia: Which One Comes Fir...
Genetics Thematic Presentation Final
1. GENE VARIANTS WITHIN THE COL1A1 GENEARE
ASSOCIATED WITH REDUCEDANTERIOR CRUCIATE
LIGAMENT INJURY IN PROFESSIONALSOCCER
PLAYERS
Krzysztof Ficek, Paweł Cieszczyk, Mariusz Kaczmarczyk, Agnieszka
Maciejewska-Karłowska, Marek Sawczuk, Jerzy Cholewinski, Agata
Leonska-Duniec, Marta Stepien-Slodkowska, Aleksandra Zarebska,
Nigel K. Stepto, David J. Bishop, Nir Eynon
3. Background – Pre-match Analysis
• Soccer is an intermittent
team sport with high
physiological demands.
• Professional players cover
8000–12,000 m during a
match, with up to 20% of the
overall distance
corresponding to maximal or
near maximal running efforts.
• Rapid movements such as
jumping and tackling are also
frequently performed by
players during a match.
4. Background – Pre-match Analysis
• Given the high physiological demands, it is not surprising that soccer
is also associated with a relatively large number of injuries
• The incidence of soccer-related injuries is estimated to be 10–35 per
1000h of exposure in adult male soccer players, with approximately
60–80% of injuries occurring in the lower extremities, most commonly
at the knee or ankle.
• The anterior cruciate ligament (ACL) rupture is one of the most
severe musculoskeletal soft tissue injuries in professional sport.
6. Background – Pre-match Analysis
• Genetic polymorphisms within the major alpha chains of the collagen
type I gene (COL1A1), which is located on chromosome 17q21, have
been shown to influence the predisposition for ACL rupture in non-
athletic cohorts.
• In particular, the functional COL1A1 Sp1 binding site polymorphism
(COL1A1 Sp1 +1245G/T, rs1800012), initially described in 1996, has
been associated with the risk for ACL injury.
7. Aim
• The aim of the study was to examine the association of the COL1A1
−1997G/T and COL1A1 Sp1 +1245G/T polymorphisms in the COL1A1 gene,
individually and as haplotypes, with ACL ruptures in professional male soccer
players.
8. Hypotheses
• Interaction between two or more polymorphisms within the COL1A1 gene may influence the
predisposition for ACL injury.
• (1) COL1A1 Sp1+1245G/T and the COL1A1 −1997G/T polymorphisms are individually
associated with the incidence of ACL rupture and;
• (2) The interaction between the COL1A1 Sp1+1245G/T and the COL1A1 −1997G/T
polymorphisms will form a haplotype that predisposes athletes to a greater risk of ACL
rupture.
16. 2.
G-T haplotype
& The COL1A1
polymorphisms
3.
TT genotype.
4.
The G-T
haplotype in
the ACL rupture
group
compared to
controls.
1.
Association
Discussi
on
17. Limitations
In general, genetic association studies must be
interpreted with caution, since there is a non-
trivial possibility of false positive results
attributable to chance, particularly in studies
involving multiple gene-trait analyses.
Additionally the present study is hampered by
small sample size, which reflects the low number
of professional soccer players, from the same
origin, who had non-contact ACL injuries.
18. Conclusion
• The COL1A1 G-T haplotype was associated with a reduced risk of ACL injury
in a group of professional soccer players. Consequently, carriers of two
copies of this haplotype may have a reduced risk of ACL injury.
• Functional analyses of COL1A1 5′ flank suggest that the G-T haplotype is
responsible for enhanced transcriptional activity of the COL1A1 gene.
19. Practical
Applications
1.
The results of the present study
will assist to understand which
genetic profiles contribute to
higher ACL injury risk.
2.
Discovering the complex
relationship between gene
variants and ACL rupture
among athletes may assist
clinicians and coaches to
optimize training, and to
reduce the risk for ACL rupture.
3.
Results suggest that
identifying the genetic
profile associated with ACL
ruptures via haplotype
analysis have become a
worthy alternative to
single-locus analysis.
20. References
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Polymorphisms: A genetic variation. Contains a different nucleotide at a certain point.
Allele: one of two or more alternative forms of a gene that arise by mutation and are found at the same place on a chromosome.
Haplotype: a combination of alleles at adjacent locations on the chromosome that are transmitted together.
Homozygous: having two identical alleles of a particular gene or genes.
Heterozygous: two different alleles of a particular gene or genes.
3. ACL ruptures are complex, multifactorial disorders determined by the interaction of extrinsic and intrinsic risk factors. The familial aggregation observed in ACL injuries has prompted researchers to investigate a possible genetic linkage.
Image representing a health ACL vs. a torn or ruptured ACL
1. 91 professional male soccer players with surgically diagnosed primary ACL ruptures who qualified for ligament reconstruction were recruited for the study. All players had non-contact ACL injuries.
- For the obvious reason that soccer teams are homogenous in term of gender, only male subjects were recruited.
2. Control group consisted of 143 apparently healthy male professional soccer players who were without any self-reported history of ligament or tendon injury
- All the participant soccer players were all in the Polish 1st division professional soccer league, with an overall training time of 14–18 h per week (7–9 training sessions a week, 2 h each training session).
- Both the ACL rupture group and the healthy controls were from the same soccer teams, of the same ethnicity (as self-reported, all Polish, East- Europeans for ≥3 generations), of similar age (ACLR group = 23 ± 3, control group = 24 ± 5), and had a comparable level of exposure to risk of ACL injury (same volume and intensity of training and match play).
3. All samples were genotyped on a Rotor-Gene real-time polymerase chain reaction (PCR) instrument (Corbett, Australia).
4. Positive and negative controls were used for the detection of both polymorphisms. The results were scored by two experienced and independent investigators who were blind to the participants’ data.
5. The genotypes between cases and controls were compared in three ways:
- A general test of association in the 2-by-3 table of phenotype-by-genotype.
- Two different modes of inheritance of minor allele were assumed: dominant and recessive
- Dominant: In which homozygotes and heterozygotes for the minor allele were pooled and compared to homozygotes for the major allele.
- Recessive: in which homozygotes and heterozygotes for the major alleles were pooled and compared to homozygotes for the minor allele.
With regards to statistics…
1. Genotype and allele frequency were analysed using X2 or Fisher exact tests
2. Allelic-based odds ratios (OR) with 95% confidence intervals (95%CI) were calculated using logistic regression analysis.
A post hoc power calculation was detected for each gene variant.
3. The programming language and environment R was used for Hardy–Weinberg and linkage disequilibrium (LD) testing (package genetics) and for the haplotype analysis (package haplo.stats).
4. Hap.score is the statistical score for haplotypes reflecting the strength of association; the positive value of Hap.score indicates increased risk of ACL injury for a particular haplotype, while the negative value indicates reduced risk. The pair-wise linkage dis-equilibrium between −1997G/T and +1245G/T was estimated by D′ and r2 . For all tests, significance was set at p < 0.05.
The genotype and allele frequencies for the COL1A1 −1997G/T and COL1A1 Sp1 +1245G/T polymorphisms of COL1A1 are shown in Table 1.
The genotype distributions for both polymorphisms met the Hardy–Weinberg expectations in both groups (ACL rupture and controls) (p > 0.2).
There were no significant differences in genotype distribution and allele frequencies of the COL1A1 −1997G/T (rs1107946) and +1245G/T (rs1800012) polymorphisms between the ACL rupture group and the control group using the 2-by-3 general test of association.
Additionally, none of the 91 participants with ACL rupture harbored the TT genotype of the COL1A1 +1245G/T (rs1800012) polymorphisms, whereas six (4.2%) TT homozygotes were present in the control group.
However, there was a trend for under-representation of the TT genotype in the ACL rupture group .
Table 2: COL1A1 −1997G/T and +1245G/T were found to be in linkage disequilibrium.
A total of 3 reconstituted haplotypes with estimated frequency >0.05 were found, and only those were evaluated for an association with ACL rupture.
The G-G (−1997G, +1245G) haplotype was the most common (frequency 67.9%). Two other haplotypes, G-T (frequency 16.9%) and T-G (frequency 15.2%), had similar frequencies. The rare T-T haplotype (frequency 0%) was not present in any of the subjects.
Table 3:
The association between the haplotypes and ACL rupture assuming three haplotype effects: additive (considering the count of a particular haplotype as 0, 1 and 2), dominant (heterozygous or homozygous carrier of a particular haplotype versus otherwise) and recessive (homozygous for a particular haplotype versus otherwise). Under the recessive mode of inheritance, haplotype analysis yielded a mild significant association with ACL rupture (p = 0.048), as two copies of the G-T haplotype conferred decreased risk of this injury.
As mentioned beforehand: the positive value of a Hap.score indicates increased risk of ACL injury for a particular haplotype, while the negative value indicates reduced risk.
1. The association between COL1A1 −1997G/T (rs1107946) and COL1A1 Sp1+1245G/T (rs1800012) and incidents of ACL ruptures in professional soccer players was examined.
2. In contrast to the initial hypotheses, The COL1A1 Sp1+1245G/T and the COL1A1 −1997G/T polymorphisms were not associated with incidents of ACL rupture; However, a novel finding in the study was that the G-T haplotype (COL1A1 −1997G, +1245T) is significantly underrepresented in the ACL rupture group compared with healthy controls (p = 0.048), suggesting that harbouring this particular haplotype may have a protective effect against ACL rupture injury.
Additionally, the −1997G/T polymorphism is not associated with incidents of ACL rupture; however it contributes to the combined influence of the COL1A1 −1997G, +1245T haplotype on the incidence of ACL ruptures
3. Suggested that the TT genotype has a possible preventive role not only in ACL rupture, but also in other soft tissue injuries.
4.The G-T haplotype (estimated frequency among subjects 14.3%) was significantly lower in the ACL rupture group compared to controls, suggesting that participants with two copies of this haplotype have a decreased risk for ACL injury.
A possible functional explanation for the aforementioned results was recently provided by Jin et al., who found a significantly higher transcriptional activity of COL1A1 gene for the haplotype G- inde-T (−1997G/T, −1663indelT, +1245G/T) compared to all other haplotypes, by using a functional luciferase gene reporter analysis.
This study suggests that higher transcriptional activity of COL1A1 results in an unusual ratio of alpha-1(I) chains relative to alpha- 2(I). However, it remains unclear if the higher transcription activity of COL1A1 has either a negative or positive effect on the incidence of soft tissue injuries, including ACL ruptures.
We believe that the results of our study are overall valid as we strictly followed the latest genotype:phenotype study recommendations,22,23 and all of the following criteria have been met: both cases and controls (athletes) clearly presented the main study phenotype (i.e., being a professional soccer players) and were equally exposed to risk of ACL rupture, participants within each cohort were both age and ethnically matched, genetic assessment was accurate and unbiased. Further, genotype distributions were in Hardy–Weinberg equilibrium (HWE) in both cases and controls and were similar to control genotype frequencies in other studies
2. However, further investigation, including evaluation of Type I/Type 3 collagen ratio, and the possible effect of microRNAs, is required to explain the relationship between COL1A1 expression and susceptibility to ACL injury.
