Elisa
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Elisa was the final laboratory experiment for the RISE program. Elisa stands for enzyme linked imunoaborsbent assay, which was the first widely used screening test for HIV. The experiment involved adding chicken antigen to wells, washing away unnecessary items, then adding goat antibody specific to the antigen. Finally, an enzymatic substrate was added to detect if the antigen tested positive or negative for HIV based on the color change in the wells. The results for the student's table were all negative while another table had some positive results.
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To learn and contribute in the professionally challenging environment in a manner that certainly benefits for man kind and enhances my skills
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DNA is uniquely coded for each individual and can therefore be used for security purposes. DNA fingerprinting and databases allow law enforcement to identify criminals and reopen cold cases. DNA is very difficult to alter or destroy and can be extracted from old blood samples or smudged fingerprints, making it a reliable source of evidence. As DNA technology advances, it is being used more widely for national security, investigations, and everyday access control like dormitories and banks. DNA databases contain profiles that can track individuals and aid in solving crimes.
Armaiz
Chronic stress from a cancer diagnosis can accelerate cancer progression by triggering further physiological and psychological reactions. Stress acts similarly to gunpowder, detonating cancer growth. While no cure exists, research investigates whether beta-blockers, usually used for other conditions, can reduce cancer progression by blocking the effects of stress. Beta-blockers may help control cancer in the future and warrant further study of their impact on specific cancer types and potential side effects.
Nano tech.
Nanotechnology involves understanding and manipulating systems less than 100 nanometers, the width of a human hair. It is widely used to create sophisticated sensors for NASA. The document describes an experiment using a sputtering deposition machine to shoot palladium and other elements onto metal surfaces to create nanostructures and nanowires, yielding some conductive results, though not enough for NASA applications.
Nano tech.
Nanotechnology involves understanding and manipulating systems less than 100 nanometers, the width of a human hair. It is widely used to create sophisticated sensors for NASA. The document describes an experiment using a sputtering deposition machine to shoot palladium and other elements onto metal surfaces to create nanostructures and nanowires, resulting in some conduction though not enough for NASA applications.
Snake venom immunology proposal
This document proposes a study to test the effects of snake venom on the immune system. The study will inject three groups of 50 test subjects with either no supplements, over-the-counter immune boosters, or diluted snake venom twice a week for 18 months. The study expects that the venom-injected group will develop a stronger immune system with more antibodies, providing greater protection against diseases and cancer. Injecting venom may also help create immunity to snake venoms to reduce snake bite fatalities.
F inal presentation ivan j.
Hybridization between animal species can both help endangered species through genetic diversity but also isolate hybrids reproductively and physically weaken them. Chimeras, organisms composed of multiple species' cells, are mostly created in labs and raise ethical issues since they could disrupt nature if human cells were fully integrated into other species. Most hybrids like ligers are infertile due to genetic incompatibilities emerging during sperm formation, and tend to be weaker than parental species due to conflicting genes, as seen in hybrid copepods' inability to respond to salt level changes.
Nano tech.
Nanotechnology involves understanding and manipulating systems less than 100 nanometers, the width of a human hair. It is widely used to create sophisticated sensors for NASA. The document describes an experiment using a sputtering deposition machine to shoot palladium and other elements onto metal surfaces to create nanowires and shapes for conduction, which produced some moderate conduction results, though not enough for NASA chips.
Snake venom immunology proposal
This document proposes a study to test the effects of snake venom on the immune system. The study will inject three groups of 50 test subjects with either no supplements, over-the-counter immune boosters, or diluted snake venom twice a week for 18 months. The study expects that the venom-injected group will develop a stronger immune system with more antibodies, providing greater protection against diseases and cancer. Injecting venom may also help prevent snake bite fatalities by inducing immunity to snake venoms.
Nano tech.
Nanotechnology involves understanding and manipulating systems less than 100 nanometers, the width of a human hair. It is widely used to create sophisticated sensors for NASA. The document describes an experiment using a sputtering deposition machine to shoot palladium and other elements onto metal surfaces to create nanowires and shapes for conduction, which produced some moderate conduction results, though not enough for NASA chips.
Snake venom-immunology33
This document proposes injecting humans with diluted snake venom to strengthen their immune systems. It hypothesizes that over time, the venom's toxins and proteins will create stronger antibodies and immunity against diseases. A methodology is outlined where humans will be divided into three test groups - a control group, a group taking immune boosters, and a group injected with diluted venom. Testing will last 1.5 years to measure the immune response in each group. The expected results are that the venom-injected group will show the strongest and most aggressive immunity compared to the other groups.
Sodis effectiveness-in-water-purification-revised1
This document summarizes a study on the effectiveness of solar disinfection (SODIS) for water purification. Water samples were collected from two brooks and exposed to UV light for varying durations, both with and without aluminum foil wrapping. While the control samples wrapped in foil remained highly contaminated, samples exposed to UV light without wrapping for the full six hours showed some disinfection of bacteria. However, cloudy weather on the day of the experiment limited direct sunlight exposure to only two hours, likely preventing full disinfection via the SODIS method. Future experiments are planned to test additional variables over different daylight conditions.
Nano tech.
Nanotechnology involves understanding and manipulating systems less than 100 nanometers, the width of a human hair. It is widely used to create sophisticated sensors for NASA. The document describes an experiment using a sputtering deposition machine to shoot palladium and other elements onto metal surfaces to create nanostructures and nanowires, resulting in some conduction though not enough for NASA applications.
Solar water disinfection in rural puerto rican counties
The document summarizes a study on using solar water disinfection (SODIS) to treat water from the Guavate River in Puerto Rico. Water samples were collected and some were exposed to sunlight in PET bottles while others were covered or not exposed. Testing found that the samples exposed to sunlight showed significant bacterial disinfection from UV rays, while covered samples showed little disinfection or even bacterial growth due to heat. The study concluded that UV rays, not heat, provided the disinfection in the SODIS process.
Nano tech.
Nanotechnology involves understanding and manipulating systems less than 100 nanometers, the width of a human hair. It is widely used to create sophisticated sensors for NASA. The document describes an experiment using a sputtering deposition machine to shoot palladium and other elements onto metal surfaces to create nanowires and shapes for conduction, which produced some moderate conduction results, though not enough for NASA chips.
Microscopy
Ferns are vascular plants that reproduce via spores rather than seeds. They contain structures called sporangia, which house many spores. There are two types of sporangia - heterosporous and homosporous, with ferns being homosporous and only producing one type of spore. Microscopy was used to examine and photograph the whole fern leaves containing sporangia at magnifications up to 80x, helping observe the spore-bearing structures.
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Sodis effectiveness-in-water-purification-revised1
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Solar water disinfection in rural puerto rican counties
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Elisa
- 1. Ivan J. Garcia<br />RISE Program<br />Elisa Paragraph<br />Elisa was the final rise laboratory and our mentor was Dr. Chiesa. Elisa stands for enzyme linked imunoaborsbent assay. This was the first widely used screening test for HIV. Basically its purpose is to detect an antibody and/or antigen in a liquid solution. During this laboratory a chicken antigen and a goat antibody were used. There are specific antibodies for specific antigens in the market today. The first step is to add a solution containing an antigen to the wells and then several washes are performed to clean away unnecessary items; the result after these first washes is an antigen on the bottom of the well. Afterwards a solution containing an antibody specific for that Antigen is added. Then two more washes are performed with a buffer. <br />The wells should now have an antigen with its specific antibody attached to it. Finally an enzymatic substrate is added to this remaining antigen-Antibody connection. This substrate is what determines whether or not that antigen was positive for HIV or not. If the welds incorporate a blue or light blue color then they were positive for HIV. Our results were completely negative, while the other table had some positive results. Overall this lab was very educational and helped me better understand how aids test were performed. This Elisa test is still a principle method for detecting HIV and maybe, as a project, this test could be modified to detect other diseases and viruses.<br />