This document provides guidance on investigating and analyzing retaliation claims under statutes enforced by the EEOC. It defines the key elements of a retaliation claim as: 1) protected activity such as opposing discrimination or participating in statutory proceedings; 2) an adverse action; and 3) a causal connection between the protected activity and adverse action. It provides examples of protected opposition and participation activities and discusses what constitutes an adverse action and how to establish a causal connection through direct or circumstantial evidence. The document also addresses seeking temporary relief and available remedies for retaliation claims.