Uploaded bySamares Biswas
Dandruff vs normal corneocytes
The document compares dandruff scales to normal corneocytes. It finds that dandruff scales contain nucleated cells while normal corneocytes contain non-nucleated cells. Samples were stained with hematoxylin and eosin and viewed under a microscope. Testing found that dandruff scales contain less glycine and similar amounts of water-soluble protein compared to normal corneocytes. Dandruff scales and corneocytes were tape stripped and extracted with water for amino acid and protein content measurements.
Dandruff vs normal corneocytes
- 1. Dandruff vs NormalCorneocytes Nucleated vs non-nucleated cells Dandruff Scales Normal Corneocytes Presence of nucleated cells Presence of non-nucleated cells The scales were stained with hematoxylin and eosin (H&E) and checked under optical fluorescence microscope
- 2. Dandruff vs NormalCorneocytes 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 Blank Strip Corneocyte Strip Dandruff Strip GlycineContent(mM) Blank Strip Corneocyte Strip Dandruff Strip 0.000 0.005 0.010 0.015 0.020 0.025 0.030 0.035 0.040 Blank Corneocytes Dandruff BSAequivalenceofprotein(mg/ml) 15minincubation 30minincubation Amino acid content Amino acids vs protein content Water-soluble protein content Dandruff scales < Corneocytes Dandruff scales Corneocytes Dandruff and corneocytes were collected by tape stripping and extract with water and divided into two parts. Part 1 was used for amino acid measurements using nynhydrin and part 2 was used for protein estimation using BCA assay.