Pembi Sherpa Lama is seeking a position that allows her to conduct research and develop innovative technologies. She has worked as a research assistant since 2014 at the Nepal Agricultural Research Council. Previously, she was a teacher from 2013-2014 and was involved with the Biotech Association of Nepal from 2009-2012. She received a Bachelor of Science degree in Biotechnology in 2013 from SANN International College. Her research projects have included work in areas such as plant tissue culture, fermentation, microbiology, molecular techniques, and more. She has skills in laboratory techniques including plant tissue culture, microbiology, molecular techniques, and chromatography. She has received scholarships and awards for her academic performance.

1. Curriculum Vitae
Miss Pembi Sherpa Lama
 Permanent address:- Listi-3,Chhyangsing,Sindhupalchowk,Bagmati,Nepal
 Contact no.:- +9779843158402, +9779741209326
 E-mail:- pembisherpalama@gmail.com
OBJECTIVE
“Work-out more, conduct effective experiment after full analysis and develop the innovative technology”
PROFESSIONAL PROFILE
 2014- Till now Research Assistantin Seed Scienceand Technology Division
(SSTD),
Nepal Agricultural Research Council (NARC)
Khumaltar,Lalitpur,Nepal
 2013- 2014 Teacher in Shree Ugrachandhi
Maddhyamik Biddhyalaya
Listi-6,Sindhupalchowk,Bagmati,Nepal
 2009-2012 Executive and public representingmember of
Biotech Association of Nepal for Research &
Development (BANRD)
EDUCATION
 2013 (3.60/4.00 CGPA) Bachelor of Science in Biotechnology
SANN International College,
Gairidhara, Kathmandu, Nepal
 2007 (64%) Intermediate of Science
Centre for Innovative Technology (CIT) Higher Secondary School,
Battisputali, Kathmandu, Nepal
 200 (80.25%) School Leaving Certificate
Tri-Star English Secondary School,
Lokanthali-16, Bhaktapur, Nepal

2. RESEARCH PROJECTS
 November 2012-September 2013 Completed project entitled “Study of Epilepsy using different
Approaches:” as a part of undergraduate thesis work.
i. Assessing level of knowledge & understanding about epilepsy among college students,
ii. Screening of C121W mutation in people living with epilepsy in Nepal &
iii. Prioritization of disease candidate gene for Temporal Lobe epilepsy using Max Planck disease
candidate prioritizer under the supervision of Miss Praphul Shakya atSANN International
College and Research Institute.
 Experimental analysis of growth possibility of potato through Apical meristem culture by varying the
millimeter size of meristem of potato atGREAT Nepal, Baneshwor and SANN international College,
Kathmandu in January-March 2012.
 Management of Cardamom plant culture at GREAT Nepal, Baneshwor in April 2012.
 Wine production and ageing at SANN International College,Kathmandu in December 2011-January 2012.
 Chicken embryo fibroblast cell culture at SANN International College,Kathmandu in December 2011.
 Vermiform composting for improvement of soil at SANN International College,Kathmandu in June 2011.
 Detection of Citrus Greening Disease in Citrus Plant leaves by CTAB method at SANN International
College, Kathmandu in April 2011.
 PCR detection of Rifampicin resistant gene (rpoB) from Mycobacterium leprae in leprosy diseased
Nepali patients at SANN International College,Kathmandu and Leprosy Mission Hospital,Lele, Lalitpur in
August 2010.
 Experimental Analysis of plant hormone Giberellin on germination of Wheat Seed embryo at SANN
International College,Gairidhara,Kathmandu in June- 2010.
 Production of virus free potato seeds of potato at SANN International College,Kathmandu in July 2009.
GRANTS
Ministry of Youth, Government of Nepal Grant: For the project, “The Virus Free Potato Seed Production
program” 2009-2010 which was pilotprojectof Biotech Association of Nepal for Research and Development
(BANRD) by Pre Basic Seed Production.
LABORATORY SKILLS
 Plant tissue culture Techniques: Callus culture, Nodal culture, meristem culture, anther culture.
 Fermentation Technology: Production of wine. I.e. Cider and food making techniques: jam- jelly
production, yoghurt production, Ketch-up production, pizza making and food grading techniques.
 Microbiology: Basic culturetechniques,bacteria isolation,identification,characterization and
transformation.
 Serological Techniques: ELISA, agglutination and immune-diffusion.
 Molecular Techniques: DNA, RNA and protein isolation,purification and quantification,PolymeraseChain
Reaction, electrophoresis,SDS-PAGE, spectrophotometer analysis.
 Chromatographic Techniques: Paper, thin layer,column and ion-exchange chromatography.

3. SEMINARS AND PRESENTATIONS
 Seminar presentation on “Study of Epilepsy using different approaches:”Assessing level of knowledge &
understanding about epilepsy among college students, Screening of C121W mutation in people living
with epilepsy in Nepal &Prioritization of disease candidate gene for Temporal Lobe epilepsy using Max
Planck disease candidate prioritizer at SANN International College,Gairidhara,Kathmandu,Nepal.
 Seminar presentation on “Computer Aided Drug Design” at SANN International College,Gairidhara,
Kathmandu, Nepal.
 Seminar presentation on “Industrial production of Streptomycin” at SANN International College,
Gairidhara,Kathmandu,Nepal.
 Seminar presentation on “Global Scenario and prospects of transgenic crops” at SANN International
College, Gairidhara,Kathmandu,Nepal.
HONORS AND AWARDS
 Merit based scholarship (University/Class Topper in First Semester) of Bachelor courses on 21st May
2009 at SANN International College,Gairidhara,Kathmandu.
 Second Prize Winning team in Inter College Bio-Tech Quiz-2012 organized by Lord Buddha Education
Foundation (LBEF) campus on 4th July 2012.
 Merit based scholarship 10+2 Level, CIT College, Battisputali,Kathmandu.
 School Topper in School Leaving Certificate(SLC) Examination in 2005.
EXTRACURRICULAR ACTIVITIES
 Active participation in differentindoors and out-door games in university Level,.
 1st prize winning team in Quizorganized by Akhil Krantikari Biddhyarthhi Union.
 Participation in Intra-Biotech QuizContest 2011 organized by BIOTECH LEARNERS’ Club on 25th May 2011
at SANN International College,Gairidhara,Kathmandu.
 Participation in TableTennis,Badminton, Cricket, etc.
 Involvement in different Education and Industrial tours.
 Active Participation in extra-curricular activities in School Level.

4. REFEREES
 Mukunda Ranjit, Ph.D.
Academician of National Academy of Scienceand Technology (NAST), Kathmandu, Nepal.
Head of Department of Biotechnology, SANN International College,Gairidhara,Kathmandu,Nepal.
Email:- ranjitmukunda@gmail.com
 Anup Sharma, Ph.D.
Lecturer of Biotechnology Department, SANN International College,Gairidhara,Kathmandu,Nepal.
Email:- a1nup@yahoo.com
 Ms. Praphul Shakya,
Thesis Supervisor
Lecturer of Biotechnology Department, SANN International College,Gairidhara, Kathmandu,Nepal.
Email:- praphulshakya@gmail.com
 Mr. Danda Pani chapagai,
The Then President of Biotech Association of Nepal for Research and Development (BANRD), Kathmandu,
Nepal.
Email:- dpchapagain@yahoo.co.uk